Back to Journals » International Journal of Nanomedicine » Volume 6

Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model

Authors Hu YL, Qi W, Han F, Shao JZ, Gao JQ

Published 14 December 2011 Volume 2011:6 Pages 3351—3359

DOI https://doi.org/10.2147/IJN.S25853

Review by Single-blind

Peer reviewer comments 4

Yu-Lan Hu1, Wang Qi1, Feng Han2, Jian-Zhong Shao3, Jian-Qing Gao1
1Institute of Pharmaceutics, College of Pharmaceutical Sciences, 2Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, 3College of Life Sciences, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China

Background: Although there are a number of reports regarding the toxicity evaluation of inorganic nanoparticles, knowledge on biodegradable nanomaterials, which have always been considered safe, is still limited. For example, the toxicity of chitosan nanoparticles, one of the most widely used drug/gene delivery vehicles, is largely unknown. In the present study, the zebrafish model was used for a safety evaluation of this nanocarrier.
Methods: Chitosan nanoparticles with two particle sizes were prepared by ionic cross-linking of chitosan with sodium tripolyphosphate. Chitosan nanoparticles of different concentrations were incubated with zebrafish embryos, and ZnO nanoparticles were used as the positive control.
Results: Embryo exposure to chitosan nanoparticles and ZnO nanoparticles resulted in a decreased hatching rate and increased mortality, which was concentration-dependent. Chitosan nanoparticles at a size of 200 nm caused malformations, including a bent spine, pericardial edema, and an opaque yolk in zebrafish embryos. Furthermore, embryos exposed to chitosan nanoparticles showed an increased rate of cell death, high expression of reactive oxygen species, as well as overexpression of heat shock protein 70, indicating that chitosan nanoparticles can cause physiological stress in zebrafish. The results also suggest that the toxicity of biodegradable nanocarriers such as chitosan nanoparticles must be addressed, especially considering the in vivo distribution of these nanoscaled particles.
Conclusion: Our results add new insights into the potential toxicity of nanoparticles produced by biodegradable materials, and may help us to understand better the nanotoxicity of drug delivery carriers.

Keywords: chitosan, nanoparticles, zebrafish embryo, nanotoxicology

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Gene-carried hepatoma targeting complex induced high gene transfection efficiency with low toxicity and significant antitumor activity

Zhao QQ, Hu YL, Zhou Y, Li N, Han M, Tang GP, Qiu F, Tabata Y, Gao JQ

International Journal of Nanomedicine 2012, 7:3191-3202

Published Date: 27 June 2012

Effective transcutaneous immunization by antigen-loaded flexible liposome in vivo

Li N, Peng LH, Chen X, Nakagawa S, Gao JQ

International Journal of Nanomedicine 2011, 6:3241-3250

Published Date: 8 December 2011

Readers of this article also read:

Supramolecular micellar nanoaggregates based on a novel chitosan/vitamin E succinate copolymer for paclitaxel selective delivery

Lian H, Sun J, Yu YP, Liu YH, Cao W, Wang YJ, Sun YH, Wang SL, He ZG

International Journal of Nanomedicine 2011, 6:3323-3334

Published Date: 12 December 2011

Electrostatic self-assembly of multilayer copolymeric membranes on the surface of porous tantalum implants for sustained release of doxorubicin

Guo X, Chen M, Feng W, Liang J, Zhao H, Tian L, Chao H, Zou X

International Journal of Nanomedicine 2011, 6:3057-3064

Published Date: 28 November 2011

Nanoparticles of carbon allotropes inhibit glioblastoma multiforme angiogenesis in ovo

Grodzik M, Sawosz E, Wierzbicki M, Orlowski P, Hotowy A, Niemiec T, Szmidt M, Mitura K, Chwalibog A

International Journal of Nanomedicine 2011, 6:3041-3048

Published Date: 25 November 2011

Antileishmanial effect of silver nanoparticles and their enhanced antiparasitic activity under ultraviolet light

Allahverdiyev AM, Abamor ES, Bagirova M, Ustundag CB, Kaya C, Kaya F, Rafailovich M

International Journal of Nanomedicine 2011, 6:2705-2714

Published Date: 3 November 2011

Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes

Samadikhah HR, Majidi A, Nikkhah M, Hosseinkhani S,

International Journal of Nanomedicine 2011, 6:2275-2283

Published Date: 12 October 2011

Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation

Poinern GEJ, Thi Le X, Shan S, Ellis T, Fenwick S, Edwards J, Fawcett D

International Journal of Nanomedicine 2011, 6:2167-2174

Published Date: 28 September 2011

Preparation and evaluation of quercetin-loaded lecithin-chitosan nanoparticles for topical delivery

Tan Q, Liu WD, Guo CY, Zhai GX

International Journal of Nanomedicine 2011, 6:1621-1630

Published Date: 10 August 2011

Synthesis of silver nanoparticles in montmorillonite and their antibacterial behavior

Shameli K, Ahmad MB, Zargar M, Yunus WM, Rustaiyan A, Ibrahim NA

International Journal of Nanomedicine 2011, 6:581-590

Published Date: 25 March 2011