Back to Journals » Drug Design, Development and Therapy » Volume 8

Topical pimecrolimus inhibits high-dose UVB irradiation-induced epidermal Langerhans cell migration, via regulation of TNF-a and E-cadherin

Authors Yin Z, Xu J, Zhou B, Wu D, Xu Y, Zhang J, Luo D

Received 8 July 2014

Accepted for publication 30 July 2014

Published 10 October 2014 Volume 2014:8 Pages 1817—1825

DOI https://doi.org/10.2147/DDDT.S70790

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Professor Shu-Feng Zhou

ZhiQiang Yin,1,* JiaLi Xu,2,* BingRong Zhou,1 Di Wu,1 Yang Xu,1 JiaAn Zhang,1 Dan Luo1

1Department of Dermatology, 2Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu, People’s Republic of China

*These authors contributed equally to this work

Background: Topical pimecrolimus has been shown to reverse epidermal CD1a+ Langerhans cell reduction induced by high-dose ultraviolet (UV)B irradiation, but the mechanism is still unclear. This study aimed to investigate the possible mechanism of the effect of pimecrolimus on high-dose UVB-irradiated epidermal Langerhans cells.
Methods: Forty human foreskin tissues were divided into four groups: control; pimecrolimus-only; UVB-only; and UVB + pimecrolimus. All tissues were cultured, and each tissue was cut into four pieces, corresponding to four time points (0 hours, 18 hours, 24 hours, and 48 hours). We collected the tissues and culture medium at each time point. The percentage of CD1a+ cells in medium was detected by flow cytometry. The tissues were detected for messenger (m)RNA and protein expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, and E-cadherin, by reverse-transcription polymerase chain reaction (PCR) and western blot.
Results: At 18 hours, 24 hours, and 48 hours, the CD1a+ cells in the culture medium of the UVB-only group and the UVB + pimecrolimus group were significantly more than in the control group, while the CD1a+ cells of the UVB + pimecrolimus group was less than of the UVB-only group. For both the UVB-only group and UVB + pimecrolimus group, TNF-α expression (by both reverse-transcription PCR and western blot) of the tissues was clearly higher and E-cadherin expression was significantly lower compared with the control group, at 18 hours, 24 hours, and 48 hours. For the UVB + pimecrolimus group, TNF-α was clearly lower and E-cadherin was significantly higher compared with the UVB-only group.
Conclusion: Topical pimecrolimus inhibited epidermal Langerhans cell migration induced by high-dose UVB irradiation, via regulation of TNF-α and E-cadherin.

Keywords: pimecrolimus, UVB, Langerhans cell, TNF-α, E-cadherin
 

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Systemic abnormalities of psoriatic patients: a retrospective study

Yin L, Xu JL, Hu YY, Johnston A, Yin ZQ

Clinical, Cosmetic and Investigational Dermatology 2016, 9:443-449

Published Date: 10 November 2016

Transient hypofibrinogenemia due to allopurinol

Yin ZQ, Xu JL, Li YQ, Xia JP, Luo D

Drug Design, Development and Therapy 2014, 8:1231-1233

Published Date: 2 September 2014

Pustular drug eruption due to Panax notoginseng saponins

Yin ZQ, Ma LW, Xu JL, Xia JP, Luo D

Drug Design, Development and Therapy 2014, 8:957-961

Published Date: 16 July 2014

Readers of this article also read:

Green synthesis of water-soluble nontoxic polymeric nanocomposites containing silver nanoparticles

Prozorova GF, Pozdnyakov AS, Kuznetsova NP, Korzhova SA, Emel’yanov AI, Ermakova TG, Fadeeva TV, Sosedova LM

International Journal of Nanomedicine 2014, 9:1883-1889

Published Date: 16 April 2014

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

A novel preparation method for silicone oil nanoemulsions and its application for coating hair with silicone

Hu Z, Liao M, Chen Y, Cai Y, Meng L, Liu Y, Lv N, Liu Z, Yuan W

International Journal of Nanomedicine 2012, 7:5719-5724

Published Date: 12 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010