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The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells

Authors Babic M, Horak D, Jendelova P, Herynek V, Proks V, Vanecek V, Lesny P, Sykova E

Received 29 November 2011

Accepted for publication 17 January 2012

Published 16 March 2012 Volume 2012:7 Pages 1461—1474

DOI https://doi.org/10.2147/IJN.S28658

Review by Single-blind

Peer reviewer comments 3

Michal Babic1,2, Daniel Horak1,2, Pavla Jendelova2,3, Vit Herynek2,4, Vladimir Proks1,2, Vaclav Vanecek2,3, Petr Lesny2,3, Eva Sykova2,3
1Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 2Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84, 3Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 4Institute for Clinical and Experimental Medicine, Videnska 1958, Prague, Czech Republic

Abstract: Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe2O3) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe2O3 surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe2O3 and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe2O3 nanoparticles with DPA-HA/γ-Fe2O3 = 0.45 (weight/weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.

Keywords: nanoparticles, dopamine, hyaluronate, cell labeling, magnetic

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