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The role of circular RNA hsa_circ_0085616 in proliferation and migration of hepatocellular carcinoma cells

Authors Li W, Zhou X, Wu X, Wei J, Huang Z

Received 3 April 2019

Accepted for publication 21 July 2019

Published 5 August 2019 Volume 2019:11 Pages 7369—7376

DOI https://doi.org/10.2147/CMAR.S211020

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Melinda Thomas

Peer reviewer comments 2

Editor who approved publication: Dr Beicheng Sun


Wenda Li,1,* Xue Zhou,2,* Xiang Wu,3 Jinxing Wei,1 Zejian Huang1

1Department of Hepatobiliary Surgery, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of China; 3Department of Hepatobiliary Pancreatic Surgery, The Second Affliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, People’s Republic of China

*These authors contributed equally to this work

Background: CircRNAs involved in the development of many diseases have been recently identified. However, the possible role of circRNAs in human hepatocellular carcinoma (HCC) remains to be investigated.
Objective: This study aimed to identify the expression of hsa_circ_0085616 in different HCC cell lines and its function in HCC tumorigenesis.
Methods: The expression of hsa_circ_0085616 was first measured in 68 pairs of HCC tissues and matched normal adjacent tissues. Further analysis was performed in different HCC cell lines and human normal hepatic cell lines. Moreover, we down- or upregulated its expression by cell transfection in vitro. Cell proliferation, migration, invasion and apoptosis were measured, and proliferation-related signaling pathway proteins were also analyzed.
Results: We found that hsa_circ_0085616 was highly expressed in all HCC cell lines compared to the normal liver cell line. Upregulation or downregulation of hsa_circ_0085616 expression could strengthen or weaken the proliferative ability of HCC cells in vitro. Moreover, the protein levels of β-catenin, p-ERK, and p-AKT, which play important roles in the typical proliferation pathway, were also affected by the expression of hsa_circ_0085616.
Conclusion: Our study indicated that hsa_circ_0085616 might be a potential biomarker and a new therapeutic target for HCC.

Keywords: circular RNA, hepatocellular carcinoma, tumor biomarker, hsa_circ_0085616

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