Back to Journals » International Journal of Nanomedicine » Volume 8 » Issue 1

The prospective protective effect of selenium nanoparticles against chromium-induced oxidative and cellular damage in rat thyroid

Authors Hassanin KMA, Abd El-Kawi SH, Hashem KS

Received 14 January 2013

Accepted for publication 22 February 2013

Published 1 May 2013 Volume 2013:8(1) Pages 1713—1720


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Kamel MA Hassanin,1 Samraa H Abd El-Kawi,2 Khalid S Hashem1

1Department of Biochemistry, Faculty of Veterinary Medicine, 2Department of Histology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt

Background: Nanotechnology has enabled researchers to synthesize nanosize particles that possess increased surface areas. Compared to conventional microparticles, it has resulted in increased interactions with biological targets.
Objective: The objective of this study was to determine the protective ability of selenium nanoparticles against hexavalent chromium-induced thyrotoxicity.
Design: Twenty male rats were used in the study, and arbitrarily assigned to four groups. Group 1 was the control group, and was given phosphate-buffered saline. Group 2 was the chromium-treated group and was given K2Cr2O7 60 µg/kg body weight intraperitoneally as a single dose on the third day of administration. Group 3 was the nano-selenium-treated group and was given selenium nanoparticles (size 3–20 nm) 0.5 mg/kg body weight intraperitoneally daily for 5 consecutive days. Group 4 was the nano-selenium chromium-treated group, which received selenium nanoparticles for 5 days and a single dose of K2Cr2O7 on the third day of administration.
Materials and methods: Blood samples were collected from rats for measuring thyroid hormones (free triiodothyronine [T3] and free thyroxine [T4]) and oxidative and antioxidant parameters (malondialdehyde [MDA], reduced glutathione [GSH], catalase, and superoxide dismutase [SOD]). Upon dissection, thyroid glands were taken for histopathological examination by using paraffin preparations stained with hematoxylin and eosin (H&E) and Masson’s trichrome. Immunohistochemical staining was performed for detecting cellular proliferation using Ki67 antibodies.
Results: The present study shows that K2Cr2O7 has a toxic effect on the thyroid gland as a result of inducing a marked oxidative damage and release of reactive oxygen species. This was shown by the significant decrease in free T3 and T4 and GSH levels, which was accompanied by significant increases in catalase, SOD, and MDA in the chromium-treated group compared to the control group. Se nanoparticles have a protective effect on K2Cr2O7-induced thyroid damage, as a result of correcting the free T3 and T4 levels and GSH, catalase, SOD, and MDA compared to the K2Cr2O7-treated group. Administration of nano-selenium alone in the nano-selenium-treated group had no toxic effect on rats’ thyroid compared to the control group. The biochemical results were confirmed by histopathological, immunohistochemical and pathomorphological studies.

Keywords: chromium, selenium nanoparticles, thyrotoxicity, protective effect

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other article by this author:

The effect of aspirin nanoemulsion on TNFα and iNOS in gastric tissue in comparison with conventional aspirin

Mahmoud FA, Hashem KS, Hussein Elkelawy AM

International Journal of Nanomedicine 2015, 10:5301-5308

Published Date: 24 August 2015

Readers of this article also read:

Neurological soft signs might be endophenotype candidates for patients with deficit syndrome schizophrenia

Albayarak Y, Akyol ES, Beyazyüz M, Baykal S, Kuloglu M

Neuropsychiatric Disease and Treatment 2015, 11:2825-2831

Published Date: 29 October 2015

Polymeric nanoparticles enhance the sonodynamic activity of meso-tetrakis (4-sulfonatophenyl) porphyrin in an in vitro neuroblastoma model

Canaparo R, Varchi G, Ballestri M, Foglietta F, Sotgiu G, Guerrini A, Francovich A, Civera P, Frairia R, Serpe L

International Journal of Nanomedicine 2013, 8:4247-4263

Published Date: 6 November 2013

Treatment of neuroblastoma and rhabdomyosarcoma using RGD-modified liposomal formulations of patupilone (EPO906)

Scherzinger-Laude K, Schönherr C, Lewrick F, Süss R, Francese G, Rössler J

International Journal of Nanomedicine 2013, 8:2197-2211

Published Date: 20 June 2013

Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes

Duan H, Feng B, Guo X, Wang J, Zhao L, Zhou G, Liu W, Cao Y, Zhang WJ

International Journal of Nanomedicine 2013, 8:2077-2084

Published Date: 5 June 2013

Planning for interprofessional change in primary health care: exploring the use of the Interprofessional Resource Centre

Patterson C, Arthur H, Peachey G, Vohra J, Price D, Pearson D, Mariani R

Advances in Medical Education and Practice 2013, 4:117-125

Published Date: 14 May 2013

Attitudes of asthmatic and nonasthmatic children to physical exercise

Dimitrakaki V, Porpodis K, Bebetsos E, Zarogoulidis P, Papaiwannou A, Tsiouda T, Tsioulis H, Zarogoulidis K

Patient Preference and Adherence 2013, 7:81-88

Published Date: 19 January 2013

Bone repair by periodontal ligament stem cell-seeded nanohydroxyapatite-chitosan scaffold

Ge S, Zhao N, Wang L, Yu M, Liu H, Song A, Huang J, Wang G, Yang P

International Journal of Nanomedicine 2012, 7:5405-5414

Published Date: 10 October 2012

New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo

Zhou T, Jia X, Li H, Wang J, Zhang H, A Y, Zhang ZZ

International Journal of Nanomedicine 2011, 6:1527-1534

Published Date: 22 July 2011

Poly (ε-caprolactone) nanofibrous ring surrounding a polyvinyl alcohol hydrogel for the development of a biocompatible two-part artificial cornea

Bakhshandeh H, Soleimani M, Shah Hosseini S, Hashemi H, Shabani I, Shafiee A, Behesht Nejad AH, Erfan M, Dinarvand R, Atyabi F

International Journal of Nanomedicine 2011, 6:1509-1515

Published Date: 14 July 2011