The Overexpression of Kinesin Superfamily Protein 2A (KIF2A) was Associated with the Proliferation and Prognosis of Esophageal Squamous Cell Carcinoma
Authors Li D, Sun H, Meng L, Li D
Received 1 February 2020
Accepted for publication 23 April 2020
Published 20 May 2020 Volume 2020:12 Pages 3731—3739
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Xueqiong Zhu
Demao Li,1 Huijie Sun,2 Linglei Meng,3 Deshang Li4
1Department of Thoracic Surgery, Xingtai People’s Hospital, Xingtai City, Hebei 054000, People’s Republic of China; 2Department of Pharmacy, Xingtai Medical College, Xingtai City, Hebei 054000, People’s Republic of China; 3Department of CT/MR, Xingtai People’s Hospital, Xingtai City, Hebei 054000, People’s Republic of China; 4Department of Laboratory, Xingtai People’s Hospital, Xingtai City, Hebei 054000, People’s Republic of China
Correspondence: Deshang Li
Department of Laboratory, Xingtai People’s Hospital, 16 Hongxing Street, Xingtai City, Hebei 054000, People’s Republic of China
Aim: Kinesin family member 2A (KIF2A) is a member of the kinesin-13 superfamily protein. KIF2A played a role in the development of many tumors. However, the role of KIF2A in esophageal squamous cell carcinoma (ESCC) remains unclear. In this study, we aimed to investigate the role of KIF2A in ESCC.
Methods: We used bioinformatics analysis to study the expression levels and prognosis of KIF2A in ESCC and normal tissues. We also used our own samples to verify the results by immunohistochemistry. Then, the biological functions of KIF2A in ESCC was studied by cell experiments and animal experiments.
Results: Both the TCGA database and our samples showed that KIF2A was relatively highly expressed in ESCC tissues and was significantly associated with disease-free survival (P =0.037) in TCGA database. Colony formation assay, CCK8 and Western blotting results showed that knockdown of KIF2A can significantly reduce colony forming ability and proliferation ability. The results of animal experiments showed that knocking down KIF2A can significantly reduce the tumor volume of mice.
Conclusion: KIF2A might be used as a prognostic factor for ESCC, and knockdown of KIF2A could inhibit ESCC proliferation in vitro and in vivo, respectively. KIF2A could serve as a potential prognostic biomarker and therapeutic target for future ESCC.
Keywords: esophageal squamous cell carcinoma, kinesin superfamily protein 2A, ESCC, KIF2A, proliferation
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