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The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma

Authors Cao K, Fang Y, Wang H, Jiang Z, Guo L, Hu Y

Received 26 November 2018

Accepted for publication 22 April 2019

Published 16 May 2019 Volume 2019:11 Pages 4505—4518

DOI https://doi.org/10.2147/CMAR.S196025

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 2

Editor who approved publication: Dr Alexandra R. Fernandes


Kun Cao,1 Yueyang Fang,1 Hao Wang,1 Zheng Jiang,1 Li Guo,2 Yong Hu1

1Department of Orthopaedics, The First Hospital Of Anhui Medical University, Hefei, People’s Republic of China; 2Department of Orthopaedics, The Second Hospital Of Shanxi Medical University, Taiyuan, People’s Republic of China

Objective: Many studies have shown that long non-coding RNAs (lncRNAs) are closely related to various cancers. This study aims to explore the roles of lncRNA HOXA11-AS in the development and progression of osteosarcoma (OS).
Methods: The expression levels of HOXA11-AS and miR-125a-5p in tumor tissues and the adjacent tissues were detected by RT-PCR method. The proliferation, migration and invasion of MG-63 and KHOS cells were determined.
Results: It was found that HOXA11-AS expression levels in OS tissues and OS cell lines were higher than those in OS adjacent tissues and normal human osteoblast cell lines. The higher expression level of HOXA11-AS was positively correlated with more severe clinical stage, distant metastasis and poor prognosis of OS. Inhibition of HOXA11-AS expression could reduce metastasis and invasion of OS cell lines. In addition, HOXA11-AS was found to be an endogenous inhibitor of miR-125a-5p, it down regulated the expression level of miR-125a-5p, and this process could promote the expression of Rab3D, the target gene of miR-125a-5p.
Conclusion: Our study elucidated the role of a new HOXA11-AS/miR-125a-5p/Rab3D regulatory pathway in promoting OS metastasis.

Keywords: osteosarcoma (OS), long non-coding RNAs (lncRNAs), HOXA11-AS, miR-125a-5p, Rab3D

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