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The Effect of Cerium Oxide on Lung Tissue in Lower Extremity Ischemia Reperfusion Injury in Sevoflurane Administered Rats

Authors Tuncay A, Sivgin V, Ozdemirkan A, Sezen SC, Boyunaga H, Kucuk A, Gunes I, Arslan M

Received 28 May 2020

Accepted for publication 21 August 2020

Published 6 October 2020 Volume 2020:15 Pages 7481—7489

DOI https://doi.org/10.2147/IJN.S263001

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Thomas J. Webster


Aydin Tuncay,1 Volkan Sivgin,2 Aycan Ozdemirkan,2 Saban Cem Sezen,3 Hakan Boyunaga,4 Aysegul Kucuk,5 Isin Gunes,6 Mustafa Arslan2

1Faculty of Medicine, Department of Cardiovascular Surgery, Erciyes University, Kayseri, Turkey; 2Faculty of Medicine, Department of Anesthesiology and Reamination, Gazi University, Ankara, Turkey; 3Faculty of Medicine, Department of Histology and Embryology, Kırıkkale University, Kırıkkale, Turkey; 4Faculty of Medicine, Department of Medical Biochemistry, Kırıkkale University, Kırıkkale, Turkey; 5Faculty of Medicine, Department of Physiology, Kütahya Health Science University, Kütahya, Turkey; 6Faculty of Medicine, Department of Anesthesiology and Reamination, Erciyes University, Kayseri, Turkey

Correspondence: Mustafa Arslan Gazi University, Faculty of Medicine
Department of Anesthesiology and Reanimation, Ankara 06510, Turkey
Tel +90 533 422 85 77
Email mustarslan@gmail.com

Introduction: We aimed to investigate the effects of cerium oxide, applied before the sevoflurane anesthesia, on lung tissue in rats with lower extremity ischemia-reperfusion (IR).
Materials and Methods: A total of 30 rats were randomly divided into five groups as; control (C), IR, cerium oxide-IR (CO-IR), IR-sevoflurane (IRS), and cerium oxide-IR-sevoflurane (CO-IRS). In the CO-IR group, 30 minutes after the injection of cerium oxide (0.5 mg/kg, intraperitoneal (i.p)), an atraumatic microvascular clamp was placed on the infrarenal abdominal aorta for 120 minutes. Then, the clamp was removed and reperfused for 120 minutes. Sevoflurane was applied in 100% oxygen at a rate of 2.3% at 4 L/min during IR. The blood samples were taken for biochemical analysis and the lung tissue samples were taken for histological analysis.
Results: Neutrophil infiltration/aggregation was significantly higher in the IR group than in the C and CO-IRS groups. The alveolar wall thickness and total lung injury scores were significantly higher in the IR group than in the C, IRS, CO-IR and CO-IRS groups.
Discussion: We determined that the administration of 0.5 mg/kg dose of cerium oxide with sevoflurane reduces the oxidative stress and corrects IR-related damage in lung tissue. Our results show that the administration of cerium oxide before IR and the administration of sevoflurane during IR have a protective effect in rats.

Keywords: cerium oxide, sevoflurane, ischemia reperfusion, biochemical analysis, histopathological analysis

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