The assessment of autofluorescence of the crystalline lens in diabetic patients and healthy controls: can it be used as a screening test?
Received 8 February 2018
Accepted for publication 17 April 2018
Published 27 June 2018 Volume 2018:12 Pages 1163—1170
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Scott Fraser
Seren Pehlivanoğlu,1 Nur Acar,2 Sinan Albayrak,1 Muharrem Karakaya,1 Ali Ofluoğlu1
1Department of Ophthalmology, School of Medicine, Yeni Yüzyıl University, Istanbul, Turkey; 2Department of Ophthalmology, School of Medicine, Acibadem University, Istanbul, Turkey
Background: Our purpose was to demonstrate if measuring lens autofluorescence (AF) with a scanning confocal biomicroscope may be used to identify subjects with undiagnosed type II diabetes mellitus (DM), and hence, for it to be used as a marker for the severity of diabetic retinopathy in diabetic patients.
Patients and methods: In this cross-sectional, comparative study, lens AF was measured with scanning confocal lens fluorescence biomicroscope in diabetic and healthy groups. Full ophthalmological examination was performed. Blood tests of fasting plasma glucose, and glycosylated hemoglobin were also analyzed. The correlation between lens AF results and blood tests was evaluated in both groups. The cutoff value for the diagnosis of DM using lens AF was investigated.
Results: The study included 191 subjects with a mean age of 52.09±6.75 years. One hundred and seven (56.0%) subjects were female, and 84 (44.0%) were male. Eighty-two (42.9%) patients had type II DM, and 109 (57.1%) subjects self-reported as normal. The fluorescence ratio (FR) values ranged from 0.09 to 0.46 (0.23±0.06) in the total group. Mean FR measurements of diabetic subjects were significantly higher (0.27±0.06) than those without DM (0.20±0.05), (p=0.001). A statistically significant correlation was found between glycosylated hemoglobin, fasting plasma glucose, and FR. The cutoff point for the FR according to the presence of DM was found to be 0.24 and above (p=0.001), with a sensitivity of 71.95% and a specificity of 80.73%.
Conclusion: Measuring AF of human lens as an indirect evidence of increased advanced glycaton end products may helpful in detecting impaired glucose metabolism. Our results show highly significant correlation between possibility of DM and FR.
Keywords: lens autofluorescence, diabetes, diabetic retinopathy, HgA1c, fasting plasma glucose, screening
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