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The Antibacterial Activity and Mechanism of Action of Luteolin Against Trueperella pyogenes

Authors Guo Y, Liu Y, Zhang Z, Chen M, Zhang D, Tian C, Liu M, Jiang G

Received 10 March 2020

Accepted for publication 16 May 2020

Published 10 June 2020 Volume 2020:13 Pages 1697—1711


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Professor Suresh Antony

Yuru Guo,1 Yan Liu,2 Zehui Zhang,1 Menghan Chen,1 Dexian Zhang,1 Chunlian Tian,1 Mingchun Liu,1 Guotuo Jiang2

1Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, People’s Republic of China; 2Dalian Sanyi Animal Medicine Co., Ltd., Dalian, Liaoning, People’s Republic of China

Correspondence: Mingchun Liu; Guotuo Jiang Tel/Fax +86 024 8848 7156
; +86 411 6688 6289

Purpose: This research aimed to investigate the antibacterial activity and potential mechanism of luteolin against T. pyogenes.
Materials and Methods: The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of luteolin against various T. pyogenes strains. The potential mechanism of action of luteolin was elucidated through testing and analysing the luteolin-induced alterations of T. pyogenes in several aspects, including cell wall, cell membrane, protein expression, nucleic acid content, topoisomerase activity and energy metabolism.
Results: The MIC values of luteolin against various T. pyogenes isolates and ATCC19411 were 78 μg/mL. The increased cell membrane permeability, destruction of cell wall integrity and TEM images after exposure to luteolin showed that the cell wall and membrane were damaged. The content of total protein and nucleic acid in T. pyogenes decreased significantly after treatment with luteolin (1/2 MIC) for 12, 24, and 36 h. Moreover, a hypochromic effect was observed in the absorption spectrum of luteolin when deoxyribonucleic acid (DNA) was added. In addition, after treatment with luteolin, a decrease in nicked or relaxed DNA content, which was catalysed by T. pyogenes-isolated DNA topoisomerase, was observed. In addition, the adenosine triphosphate (ATP) content in cells and the activity of succinate dehydrogenase (SDH) both decreased when T. pyogenes was exposed to different concentrations (1/4 MIC, 1/2 MIC, 1 MIC, 2 MIC) of luteolin for 1 h.
Conclusion: Luteolin showed distinct antibacterial activity against T. pyogenes by multiple actions, which mainly include destroying the integrity of the cell wall and cell membrane, influencing the expression of proteins, inhibiting nucleic acid synthesis, and interfering with energy metabolism.

Keywords: luteolin, Trueperella pyogenes, antibacterial activity, antibacterial mechanism

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