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Targeting ZEB2 By microRNA-129 In Non-Small Cell Lung Cancer Suppresses Cell Proliferation, Invasion And Migration Via Regulating Wnt/β-Catenin Signaling Pathway And Epithelial–Mesenchymal Transition

Authors Li X, Li C, Bi H, Bai S, Zhao L, Zhang J, Qi C

Received 29 May 2019

Accepted for publication 2 October 2019

Published 5 November 2019 Volume 2019:12 Pages 9165—9175

DOI https://doi.org/10.2147/OTT.S217536

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Ms Rachel Predeepa

Peer reviewer comments 3

Editor who approved publication: Dr Sanjeev Srivastava


Xingtao Li,1 Chunhong Li,2 Hongmei Bi,3 Shufang Bai,4 Lin Zhao,5 Jing Zhang,6 Chunhui Qi7

1Department of Clinical Laboratory, Jinan City People’s Hospital, Laiwu 271100, People’s Republic of China; 2Department of Public Health, Jinan Zhangqiu District Hospital of TCM, Jinan 250200, People’s Republic of China; 3Department of Respiratory Medicine, The Third People’s Hospital of Qingdao, Qingdao 266041, People’s Republic of China; 4Department of Ultrasound, The People’s Hospital of Zhangqiu Area, Jinan 250200, People’s Republic of China; 5Department of Respiratory Medicine, People’s Hospital of Rizhao, Rizhao 276826, People’s Republic of China; 6Department of Cardiothoracic Vascular Surgery, People’s Hospital of Rizhao, Rizhao 276826, People’s Republic of China; 7Department of Pharmacy, Weifang People’s Hospital, Weifang 261041, People’s Republic of China

Correspondence: Chunhui Qi
Department of Pharmacy, Weifang People’s Hospital, 151 Guangwen street, Weifang 261041, People’s Republic of China
Tel +8600634-6279088
Email bwurtyu@163.com

Introduction: Non-small cell lung cancer (NSCLC) is a common cause of deaths all over the world. Emerging evidence has indicated that microRNA (miR) play key roles in NSCLC progression. We aimed to determine the functions of miR-129 in NSCLC. miR-129 was dramatically downregulated in NSCLC tissue samples and cells. The decreased miR-129 was found to be associated with poorer prognosis and malefic phenotype of NSCLC patients. We demonstrated that miR-129 upregulation could inhibit NSCLC cell growth. Furthermore, we also sought the molecular mechanism by which miR-129 repressed NSCLC development.
Methods: QRT-PCR was applied to detect the expressions of miR-129 in 51 pairs of NSCLC tissue samples. We further performed the Kaplan–Meier analysis to determine the association between miR-129 expressions and the survival rate of NSCLC patients. We then measured the expression levels of miR-129 in NSCLC cell lines. After that, MTT assays were performed to determine the influence of miR-129 on A549 cell proliferation. Transwell assay was then conducted to explore the biological functions of miR-129 in invasion and migration of NSCLC cells.
Results: Results showed that ZEB2 was directly targeted by miR-129 in NSCLC cell lines. Moreover, miR-129 restoration could inhibit EMT and Wnt/β-catenin in NSCLC cell lines.
Conclusion: In short, all these results indicated that miR-129/ZEB2 axis maybe a useful diagnostic and prognostic biomarker for NSCLC treatment.

Keywords: miR-129, ZEB2, NSCLC, Wnt/β-catenin, EMT

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