Targeting ZEB2 By microRNA-129 In Non-Small Cell Lung Cancer Suppresses Cell Proliferation, Invasion And Migration Via Regulating Wnt/β-Catenin Signaling Pathway And Epithelial–Mesenchymal Transition
Authors Li X, Li C, Bi H, Bai S, Zhao L, Zhang J, Qi C
Received 29 May 2019
Accepted for publication 2 October 2019
Published 5 November 2019 Volume 2019:12 Pages 9165—9175
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Sanjeev Srivastava
Xingtao Li,1 Chunhong Li,2 Hongmei Bi,3 Shufang Bai,4 Lin Zhao,5 Jing Zhang,6 Chunhui Qi7
1Department of Clinical Laboratory, Jinan City People’s Hospital, Laiwu 271100, People’s Republic of China; 2Department of Public Health, Jinan Zhangqiu District Hospital of TCM, Jinan 250200, People’s Republic of China; 3Department of Respiratory Medicine, The Third People’s Hospital of Qingdao, Qingdao 266041, People’s Republic of China; 4Department of Ultrasound, The People’s Hospital of Zhangqiu Area, Jinan 250200, People’s Republic of China; 5Department of Respiratory Medicine, People’s Hospital of Rizhao, Rizhao 276826, People’s Republic of China; 6Department of Cardiothoracic Vascular Surgery, People’s Hospital of Rizhao, Rizhao 276826, People’s Republic of China; 7Department of Pharmacy, Weifang People’s Hospital, Weifang 261041, People’s Republic of China
Correspondence: Chunhui Qi
Department of Pharmacy, Weifang People’s Hospital, 151 Guangwen street, Weifang 261041, People’s Republic of China
Introduction: Non-small cell lung cancer (NSCLC) is a common cause of deaths all over the world. Emerging evidence has indicated that microRNA (miR) play key roles in NSCLC progression. We aimed to determine the functions of miR-129 in NSCLC. miR-129 was dramatically downregulated in NSCLC tissue samples and cells. The decreased miR-129 was found to be associated with poorer prognosis and malefic phenotype of NSCLC patients. We demonstrated that miR-129 upregulation could inhibit NSCLC cell growth. Furthermore, we also sought the molecular mechanism by which miR-129 repressed NSCLC development.
Methods: QRT-PCR was applied to detect the expressions of miR-129 in 51 pairs of NSCLC tissue samples. We further performed the Kaplan–Meier analysis to determine the association between miR-129 expressions and the survival rate of NSCLC patients. We then measured the expression levels of miR-129 in NSCLC cell lines. After that, MTT assays were performed to determine the influence of miR-129 on A549 cell proliferation. Transwell assay was then conducted to explore the biological functions of miR-129 in invasion and migration of NSCLC cells.
Results: Results showed that ZEB2 was directly targeted by miR-129 in NSCLC cell lines. Moreover, miR-129 restoration could inhibit EMT and Wnt/β-catenin in NSCLC cell lines.
Conclusion: In short, all these results indicated that miR-129/ZEB2 axis maybe a useful diagnostic and prognostic biomarker for NSCLC treatment.
Keywords: miR-129, ZEB2, NSCLC, Wnt/β-catenin, EMT
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]