Back to Journals » International Journal of Nanomedicine » Volume 4

Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells

Authors Baoan Chen, Yiqiong Liang, Weiwei Wu, et al

Published 10 November 2009 Volume 2009:4 Pages 251—259

DOI https://doi.org/10.2147/IJN.S7932

Review by Single-blind

Peer reviewer comments 2

Baoan Chen1,*, Yiqiong Liang1,*, Weiwei Wu1, Jian Cheng1, Guohua Xia1, Feng Gao1, Jiahua Ding1, Chong Gao1, Zeye Shao1, Guohong Li1, Wenji Chen1, Wenlin Xu2, Xinchen Sun3, Lijie Liu4, Xiaomao Li5, Xuemei Wang6

1Department of Hematology; 3Department of Oncology, The Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People’s Republic of China; 2Department of Hematology, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang, People’s Republic of China; 4Institution of Physiology, Southeast University, Nanjing, People’s Republic of China; 5Department of Physics, University of Saarland, Saarbruechen, Germany; 6State Key Lab of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, Peoples Republic of China; *These authors have contributed equally to this work

Abstract: Gambogic acid (GA) has a significant anticancer effect on a wide variety of solid tumors. Recently, many nanoparticles have been introduced as drug-delivery systems to enhance the efficiency of anticancer drug delivery. The aim of this study was to investigate the potential benefit of combination therapy with GA and magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4). The proliferation of K562 cells and their cytotoxicity were evaluated by MTT assay. Cell apoptosis was observed and analyzed by microscope and flow cytometry, respectively. Furthermore, realtime polymerase chain reaction and Western blotting analyses were performed to examine gene transcription and protein expression, respectively. The results showed that MNPs-Fe3O4 dramatically enhanced GA-induced cytotoxicity and apoptosis in K562 cells. The typical morphological features of apoptosis treated with GA and MNPs-Fe3O4 were observed under an optical microscope and a fluorescence microscope, respectively. The transcription of caspase-3 and bax gene in the group treated with GA and MNPs-Fe3O4 was higher than that in the GA-alone group or MNPs-Fe3O4-alone group, but the transcription of bcl-2, nuclear factor-κB, and survivin degraded as did the expression of corresponding proteins in K562 cells. Our data suggests a potential clinical application of a combination of GA and MNPs-Fe3O4 in leukemia therapy.

Keywords: gambogic acid, magnetic nanoparticles of Fe3O4, traditional Chinese medicine, K562 leukemia cells, apoptosis

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF] 

 

Other articles by this author:

Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells

Tian L, Chen BA, Cheng J, Guo QL

OncoTargets and Therapy 2015, 8:2285-2290

Published Date: 26 August 2015

Reversal of multidrug resistance by cisplatin-loaded magnetic Fe3O4 nanoparticles in A549/DDP lung cancer cells in vitro and in vivo

Li K, Chen B, Xu L, Feng J, Xia G, Cheng J, Wang J, Gao F, Wang X

International Journal of Nanomedicine 2013, 8:1867-1877

Published Date: 9 May 2013

Synergistic effect of a combination of nanoparticulate Fe3O4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

Fang L, Chen B, Liu S, Wang R, Hu S, Xia G, Tian Y, Cai X

International Journal of Nanomedicine 2012, 7:4109-4118

Published Date: 30 July 2012

Effect of magnetic nanoparticles of Fe3O4 and wogonin on the reversal of multidrug resistance in K562/A02 cell line

Cheng J, Cheng L, Chen B, Xia G, Gao C, Song H, Bao W, Guo Q, Zhang H, Wang X

International Journal of Nanomedicine 2012, 7:2843-2852

Published Date: 8 June 2012

Multifunctional magnetic Fe3O4 nanoparticles combined with chemotherapy and hyperthermia to overcome multidrug resistance

Ren Y, Zhang H, Chen B, Cheng J, Cai X, Liu R, Xia G, Wu W, Wang S, Ding J, Gao C, Wang J, Bao W, Wang L, Tian L, Song H, Wang X

International Journal of Nanomedicine 2012, 7:2261-2269

Published Date: 3 May 2012

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Wang L, Zhang HJ, Chen BA, Xia GH, Wang S, Cheng J, Shao Z, Gao C, Bao W, Tian L, Ren YY, Xu PP, Cai XH, Liu R, Wang XM

International Journal of Nanomedicine 2012, 7:789-798

Published Date: 14 February 2012

Daunorubicin-TiO2 nanocomposites as a “smart” pH-responsive drug delivery system

Zhang H, Wang C, Chen B, Wang X

International Journal of Nanomedicine 2012, 7:235-242

Published Date: 12 January 2012

Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models

Cheng J, Zhou Y, Chen B, Wang J, Xia G, Jin N, Ding J, Gao C, Chen G, Miao Y, Li W, Liu Z, Wang X

International Journal of Nanomedicine 2011, 6:2183-2189

Published Date: 3 October 2011

A promising strategy for overcoming MDR in tumor by magnetic iron oxide nanoparticles co-loaded with daunorubicin and 5-bromotetrandrin

Cheng J, Wang J, Chen BA, Xia GH, Cai XH, Liu R, Ren YY, Bao W, Wang XM

International Journal of Nanomedicine 2011, 6:2123-2131

Published Date: 27 September 2011

Effect of magnetic Fe3O4 nanoparticles with 2-methoxyestradiol on the cell-cycle progression and apoptosis of myelodysplastic syndrome cells

Xia G, Chen B, Ding J, Gao C, Lu H, Shao Z, Gao F, Wang X

International Journal of Nanomedicine 2011, 6:1921-1927

Published Date: 8 September 2011

Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells

Wang Y, Han Y, Yang Y, Yang J, Guo X, Zhang J, Pan L, Xia G, Chen B

International Journal of Nanomedicine 2011, 6:1185-1192

Published Date: 9 June 2011

The changes of T lymphocytes and cytokines in ICR mice fed with Fe3O4 magnetic nanoparticles

Wang J, Chen B, Jin N, Xia G, Chen Y, Zhou Y, Cai X, Ding J, Li X, Wang X

International Journal of Nanomedicine 2011, 6:605-610

Published Date: 1 April 2011

Synthesis and antitumor efficacy of daunorubicin-loaded magnetic nanoparticles

Jun Wang, Baoan Chen, Jian Chen, et al

International Journal of Nanomedicine 2011, 6:203-211

Published Date: 24 January 2011

Biocompatibility of Fe3O4/DNR magnetic nanoparticles in the treatment of hematologic malignancies

Weiwei Wu, Baoan Chen, Jian Cheng, et al

International Journal of Nanomedicine 2010, 5:1079-1084

Published Date: 2 December 2010

Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells

Hongmei Jing, Jing Wang, Ping Yang, et al

International Journal of Nanomedicine 2010, 5:999-1004

Published Date: 19 November 2010

Pharmacokinetic parameters and tissue distribution of magnetic Fe3O4 nanoparticles in mice

Jun Wang, Yue Chen, Baoan Chen, et al

International Journal of Nanomedicine 2010, 5:861-866

Published Date: 13 October 2010

The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice

Bao-An Chen, Nan Jin, Jun Wang, et al

International Journal of Nanomedicine 2010, 5:593-599

Published Date: 10 August 2010

Effect of Fe3O4-magnetic nanoparticles on acute exercise enhanced KCNQ1 expression in mouse cardiac muscle

Lijie Liu, Baoan Chen,Feixiang Teng, et al

International Journal of Nanomedicine 2010, 5:109-116

Published Date: 25 February 2010

The reversal effect of magnetic Fe3O4 nanoparticles loaded with cisplatin on SKOV3/DDP ovarian carcinoma cells

Zhi Jiang, Bao-An Chen, Guo-Hua Xia, Qiang Wu, et al.

International Journal of Nanomedicine 2009, 4:107-114

Published Date: 30 April 2009

Reversal of multidrug resistance by magnetic Fe3O4 nanoparticle copolymerizating daunorubicin and 5-bromotetrandrine in xenograft nude-mice

Baoan Chen, Jian Cheng, Yanan Wu, Feng Gao, Wenlin Xu, et al

International Journal of Nanomedicine 2009, 4:73-78

Published Date: 18 March 2009

Synergistic effect of the combination of nanoparticulate Fe3O4 and Au with daunomycin on K562/A02 cells

Bao-An Chen, Yong-Yuan Dai, Xue-Mei Wang, Ren-Yun Zhang, Wen-Lin Xu, et al

International Journal of Nanomedicine 2008, 3:343-350

Published Date: 12 September 2008

Readers of this article also read:

Molecular targets in arthritis and recent trends in nanotherapy

Roy K, Kanwar RK, Kanwar JR

International Journal of Nanomedicine 2015, 10:5407-5420

Published Date: 26 August 2015

Is increasing the dose of Entecavir effective in partial virological responders?

Erturk A, Adnan Akdogan R, Parlak E, Cure E, Cumhur Cure M, Ozturk C

Drug Design, Development and Therapy 2014, 8:621-625

Published Date: 29 May 2014

Photodynamic therapy of a 2-methoxyestradiol tumor-targeting drug delivery system mediated by Asn-Gly-Arg in breast cancer

Shi J, Wang Z, Wang L, Wang H, Li L, Yu X, Zhang J, Ma R, Zhang Z

International Journal of Nanomedicine 2013, 8:1551-1562

Published Date: 19 April 2013

Controlled-release approaches towards the chemotherapy of tuberculosis

Saifullah B, Hussein MZ, Hussein Al Ali SH

International Journal of Nanomedicine 2012, 7:5451-5463

Published Date: 12 October 2012

Systematic in-vitro evaluation of the NCI/NIH Developmental Therapeutics Program Approved Oncology Drug Set for the identification of a candidate drug repertoire for MLL-rearranged leukemia

Hoeksema KA, Jayanthan A, Cooper T, Gore L, Trippett T, Boklan J, Arceci RJ, Narendran A

OncoTargets and Therapy 2011, 4:149-168

Published Date: 5 September 2011

Treatment of acute otitis externa with ciprofloxacin otic 0.2% antibiotic ear solution

Mösges R, Nematian-Samani M, Eichel A

Therapeutics and Clinical Risk Management 2011, 7:325-326

Published Date: 27 July 2011