Subacute toxicity study in Wistar rats fed with StemEnhanceTM, an extract from Aphanizomenon flos-aquae
Levent Dirikolu1, Thushara Chakkath1, Susan Ball-Kell2, Christy Elamma2, Timothy M Fan3, David J Schaeffer1
1Department of Comparative Biosciences, 2Veterinary Diagnostic Laboratory, 3Comparative Oncology Research Laboratory, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA
Abstract: This study evaluated the safety (absence of toxicity) of StemEnhance™, an extract of the blue-green alga Aphanizomenon flos-aquae that is used as a health supplement. Groups of 12 rats of each sex were given either 5% glycerin in water (control) or 600 mg/kg of StemEnhance prepared in 5% glycerin in water for 2 weeks by oral gavage followed by 2 weeks of observation. The administration of StemEnhance had no effect on behavior, food and water intake, growth, or survival. Values at the end of dosing and observation periods did not reveal differences between treated and control groups for hematology and clinical chemistry. There were no significant differences in the gross and histopathology of the reproductive organs in either males or females. Sperm motility parameters were similar for control and treated males. Our results show that StemEnhance at doses ~20 times the maximum label-recommended daily dose did not produce adverse effects in Wistar rats after subacute treatment.
Keywords: algal toxicology, blue-green algae, cyanobacteria, Aphanizomenon flos-aquae
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