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Spectrophotometric study of the interaction between arsenic and bovine serum albumin

Authors Curvale R, Debattista NB, Pappano NB, Gonzalez D, Curvale DA

Received 15 April 2015

Accepted for publication 19 May 2015

Published 11 August 2015 Volume 2015:6 Pages 131—135

DOI https://doi.org/10.2147/RRB.S86486

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Zvi Kelman

Rolando A Curvale,1 Nora B Debattista,1 Nora B Pappano,1 Diana M Gonzalez,2 Daniela A Curvale2

1Area of Physical Chemistry, 2Area of Pharmacology and Toxicology, Chemistry, Biochemistry and Pharmacy Faculty, San Luis National University, San Luis, Argentina

Abstract: Argentina is one of the countries that are most widely affected by chronic endemic regional hydroarsenicism, a syndrome caused by the constant consumption of water polluted with arsenic at higher levels. In this study, the interaction of bovine serum albumin with arsenite and arsenate was analyzed employing UV-Vis spectroscopy, considering its low cost and greater availability. The number of binding sites, binding constants, and thermodynamic parameters – ΔG°, ΔH°, and ΔS° –were determined. The results obtained indicate that the interaction of bovine serum albumin with arsenate is ionic, while that with arsenite is hydrophobic.

Keywords: arsenic, bovine serum albumin, UV spectroscopy, thermodynamic parameters

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