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Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9

Authors Jian C, Zou C, Xu N, Chen G, Zou D

Received 11 May 2018

Accepted for publication 5 July 2018

Published 7 September 2018 Volume 2018:11 Pages 39—41

DOI https://doi.org/10.2147/SCCAA.S173852

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Dr Bernard Binetruy


Chongdong Jian,1,* Cuihua Zou,1,* Ning Xu,2 Guoying Chen,2 Donghua Zou2

1Youjiang Medical University for Nationalities, Baise, Guangxi 533000, People’s Republic of China; 2Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, People’s Republic of China

*These authors contributed equally to this work

Objective: To explore the role and mechanism of Sirt1 in protecting neural stem cells (NSCs) from apoptosis.
Materials and methods: Transfection was used to overexpress Sirt1 in rat NSCs. The effect of Sirt1 overexpression on camptothecin-induced apoptosis of NSCs was evaluated. Western blotting was used to examine the expression of Sirt1, cleaved caspase-3, and acetylated histone 3K9.
Results: Overexpression of Sirt1 in NSCs decreased the cleavage of caspase-3 and acetylation of histone 3K9.
Conclusion: Sirt1 may protect NSCs from apoptosis by decreasing the acetylation of histone 3 on K9.

Keywords: neural stem cells, apoptosis, Sirt1, caspase-3, acetylated histone 3K9

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