Menu
Back to Journals » International Journal of Nanomedicine » Volume 8 » Issue 1
Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
Authors Li C, Fu R, Yu C, Li Z, Guan H, Hu D, Zhao D, Lu L
Received 17 July 2013
Accepted for publication 24 August 2013
Published 1 November 2013 Volume 2013:8(1) Pages 4131—4145
DOI https://doi.org/10.2147/IJN.S51679
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 4
Chenwen Li,1,* Ruoqiu Fu,1,* Caiping Yu,1 Zhuoheng Li,1 Haiyan Guan,1 Daqiang Hu,1 Dehua Zhao,2 Laichun Lu1
1Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 2College of Pharmacy, Chongqing Medical University, Chongqing, People's Republic of China
*These authors contributed equally to this work
Abstract: In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag+ was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130–192 nm. The diameters of the AgNPs were in the range of 15–22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing.
Keywords: preclinical study, wound dressing, electrospinning, silver nanoparticles, poly(vinyl alcohol), chitosan oligosaccharides
© 2013 The Author(s). This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License.
By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.