Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Authors Kim KJ, Joe YA, Kim MK, Lee SJ, Ryu YH, Cho D, Rhie JW

Received 29 July 2014

Accepted for publication 27 October 2014

Published 24 March 2015 Volume 2015:10(1) Pages 2261—2272


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Lei Yang

Ki Joo Kim,1,2 Young Ae Joe,3 Min Kyoung Kim,1,2 Su Jin Lee,1 Yeon Hee Ryu,1,2 Dong-Woo Cho,4,5 Jong Won Rhie1,2

1Department of Plastic Surgery, College of Medicine, 2Department of Molecular Biomedicine, 3Cancer Research Institute and Department of Medical Lifescience, The Catholic University of Korea, Seoul, Republic of Korea; 4Department of Mechanical Engineering, Pohang University of Science and Technology, Gyeongbuk, Republic of Korea; 5Department of Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Gyeongbuk, Republic of Korea

Background: Silicon dioxide composites have been found to enhance the mechanical properties of scaffolds and to support growth of human adipose tissue-derived stem cells (hADSCs) both in vitro and in vivo. Silica (silicon dioxide alone) exists as differently sized particles when suspended in culture medium, but it is not clear whether particle size influences the beneficial effect of silicon dioxide on hADSCs. In this study, we examined the effect of different sized particles on growth and mitogen-activated protein kinase signaling in hADSCs.
Methods: Silica gel was prepared by a chemical reaction using hydrochloric acid and sodium silicate, washed, sterilized, and suspended in serum-free culture medium for 48 hours, and then sequentially filtered through a 0.22 µm filter (filtrate containing nanoparticles smaller than 220 nm; silica NPs). hADSCs were incubated with silica NPs or 3 µm silica microparticles (MPs), examined by transmission electron microscopy, and assayed for cell proliferation, apoptosis, and mitogen-activated protein kinase signaling.
Results: Eighty-nine percent of the silica NPs were around 50–120 nm in size. When hADSCs were treated with the study particles, silica NPs were observed in endocytosed vacuoles in the cytosol of hADSCs, but silica MPs showed no cell entry. Silica NPs increased the proliferation of hADSCs, but silica MPs had no significant effect in this regard. Instead, silica MPs induced slight apoptosis. Silica NPs increased phosphorylation of extracellular signal-related kinase (ERK)1/2, while silica MPs increased phosphorylation of p38. Silica NPs had no effect on phosphorylation of Janus kinase or p38. Pretreatment with PD98059, a MEK inhibitor, prevented the ERK1/2 phosphorylation and proliferation induced by silica NPs.
Conclusion: Scaffolds containing silicon dioxide for tissue engineering may enhance cell growth through ERK1/2 activation only when NPs around 50–120 nm in size are included, and single component silica-derived NPs could be useful for bioscaffolds in stem cell therapy.

Keywords: particle, ceramic, biomaterial, mesenchymal stem cells, ERK

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]