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Silencing Of hsa_circ_0008450 Represses Hepatocellular Carcinoma Progression Through Regulation Of microRNA-214-3p/EZH2 Axis

Authors Lin T, Dai Y, Guo X, Chen W, Zhao J, Cao L, Wu Z

Received 10 July 2019

Accepted for publication 9 October 2019

Published 24 October 2019 Volume 2019:11 Pages 9133—9143

DOI https://doi.org/10.2147/CMAR.S222716

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Melinda Thomas

Peer reviewer comments 3

Editor who approved publication: Dr Beicheng Sun


Tianyu Lin,1,* Yi Dai,1,* Xinli Guo,2 Wenchao Chen,1 Jiangang Zhao,3 Liping Cao,1 Zhengrong Wu1

1Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, People’s Republic of China; 2Department of Operating Room, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, People’s Republic of China; 3Department of General, Visceral and Tumor Surgery, University Hospital Cologne, Cologne 50934, Germany

*These authors contributed equally to this work

Correspondence: Liping Cao; Zhengrong Wu
Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, No. 3, Qingchun Road, Hangzhou 310000, People’s Republic of China
Tel +86-571-86006961
Fax +86-571-86006961
Email caolipingzju@zju.edu.cn; 2202005@zju.edu.cn

Purpose: Circular RNA (circRNA) hsa_circ_0008450 has been shown to be up-regulated in hepatocellular carcinoma (HCC). However, the functional role of hsa_circ_0008450 and its molecular mechanism are still unknown.
Patients and methods: We used qRT-PCR and Western blot to examine the expression levels of hsa_circ_0008450, microRNA-214-3p (miR-214-3p), and enhancer of zeste homolog 2 (EZH2) protein. CCK8 assay and wound healing assay were used to detect cell viability and cell migration capability. Cell apoptosis was assessed by flow cytometry. Luciferase reporter assay was used to explore the interaction among hsa_circ_0008450, miR-214-3p, and EZH2.
Results: hsa_circ_0008450 was significantly increased in HCC tissues and cells. Furthermore, knockdown of hsa_circ_0008450 in HCC cells inhibited cell proliferation, invasion, and migration. Mechanically, hsa_circ_0008450 promoted the expression of EZH2 protein through sponging miR-214-3p. Knockdown of circ_0008450 suppressed tumorigenesis of HCC cells in vivo.
Conclusion: Knockdown of hsa_circ_0008450 inhibits HCC progression by regulating miR-214-3p/EZH2 axis. This study suggests that hsa_circ_0008450 may serve as a novel target for the treatment of HCC.

Keywords: circular RNAs, hepatocellular carcinoma, hsa_circ_0008450, miR-214-3p, enhancer of zeste homolog 2

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