SIK1-LNC represses the proliferative, migrative, and invasive abilities of lung cancer cells
Received 11 February 2018
Accepted for publication 30 May 2018
Published 19 July 2018 Volume 2018:11 Pages 4197—4206
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Justinn Cochran
Peer reviewer comments 3
Editor who approved publication: Professor Jianmin Xu
Liu Yang,1,* Nianlin Xie,2,* Jingyu Huang,3,* Hu Huang,4,* Shaogan Xu,5 Zhigang Wang,4 Jun Cai6
1Department of Cancer Biotherapy Center, Hubei Cancer Hospital, Wuhan 430079, Hubei, People’s Republic of China; 2Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, Shaanxi, People’s Republic of China; 3Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, People’s Republic of China; 4Department of Oncology, The 161th Hospital of PLA, Wuhan, Hubei 430010, People’s Republic of China; 5Department of Thoracic Surgery, The 161th Hospital of PLA, Wuhan, Hubei 430010, People’s Republic of China; 6Department of Oncology, First Affiliated Hospital of Yangtze University, Jingzhou 434000, Hubei, People’s Republic of China
*These authors contributed equally to this work
Background: Discussions regarding the correlations between long non-coding RNAs (lncRNAs) and cancers have dominated research in recent years. SIK1-LNC, a type of lncRNA and adjacent to salt-inducible kinases 1 (SIK1), has been found aberrantly expressed in lung cancer. However, its functional role in lung cancer remains largely unknown.
Purpose: In this study, we aimed to explore the association between SIK1-LNC expression and SIK1 in lung cancer cells and further identify the impact of SIK1-LNC on the proliferation, migration invasion of lung cancer cells.
Patients and methods: Of the 30 patients with non-small-cell lung carcinoma from Zhongnan Hospital of Wuhan University, RT-qPCR was performed to detect SIK1 and SIK1-LNC expressions in patients’ samples. Overexpression and knockdown experiments were conducted to analyze the SIK1 and SIK1-LNC expressions in lung cancer cell lines. CCK-8, Brdu, scratch wound-healing, and Transwell assays were respectively employed to evaluate the proliferative, migrative, and invasive abilities of lung cancer cells.
Results: Both SIK1-LNC and SIK1 expression levels were evidently downregulated in 30 lung cancer tissues. SIK1-LNC expression was bound up with clinicopathologic features, including lymph node metastasis and distant metastasis. SIK1 expression showed a positive tendency with SIK1-LNC expression in lung cancer cells. SIK1-LNC exerted a significant repression on cell proliferatiive, miogrative and invasive abilities of lung cancer cells.
Conclusion: Our findings suggested that SIK1-LNC may act as a novel biomarker and therapeutic target for lung cancer.
Keywords: lung cancer, SIK1-LNC, SIK1, proliferation, invasion, metastasis
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