Self-assembling surfactant-like peptide A6K as potential delivery system for hydrophobic drugs
Received 24 July 2014
Accepted for publication 1 December 2014
Published 23 January 2015 Volume 2015:10(1) Pages 847—858
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 4
Editor who approved publication: Dr Lei Yang
Yongzhu Chen,1 Chengkang Tang,2 Jie Zhang,2 Meng Gong,3 Bo Su,2 Feng Qiu4
1Periodical Press, 2Core Facility of West China Hospital, 3Laboratory of Endocrinology and Metabolism, West China Hospital, 4Laboratory of Anaesthesia and Critical Care Medicine, Translational Neuroscience Centre, West China Hospital, Sichuan University, Chengdu, People’s Republic of China
Background: Finding a suitable delivery system to improve the water solubility of hydrophobic drugs is a critical challenge in the development of effective formulations. In this study, we used A6K, a self-assembling surfactant-like peptide, as a carrier to encapsulate and deliver hydrophobic pyrene.
Methods: Pyrene was mixed with A6K by magnetic stirring to form a suspension. Confocal laser scanning microscopy, transmission electron microscopy, dynamic light scattering, atomic force microscopy, fluorescence, and cell uptake measurements were carried out to study the features and stability of the nanostructures, the state and content of pyrene, as well as the pyrene release profile.
Results: The suspension formed contained pyrene monomers trapped in the hydrophobic cores of the micellar nanofibers formed by A6K, as well as nanosized pyrene crystals wrapped up and stabilized by the nanofibers. The two different encapsulation methods greatly increased the concentration of pyrene in the suspension, and formation of pyrene crystals wrapped up by A6K nanofibers might be the major contributor to this effect. Furthermore, the suspension system could readily release and transfer pyrene into living cells.
Conclusion: A6K could be further exploited as a promising delivery system for hydrophobic drugs.
Keywords: pyrene, self-assembling peptide, micelles, nanofibers, drug delivery
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