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Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro

Authors Norouzi R, Ataei A, Hejazy M, Noreddin A, El Zowalaty ME

Received 24 August 2019

Accepted for publication 25 October 2019

Published 17 February 2020 Volume 2020:15 Pages 1095—1100

DOI https://doi.org/10.2147/IJN.S228538

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Roghayeh Norouzi,1 Amin Ataei,1 Marzie Hejazy,2 Ayman Noreddin,3,4 Mohamed Ezzat El Zowalaty5

1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 2Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 3Infectious Diseases and Anti-Infective Therapy Research Group, Sharjah Medical Research Institute and College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates; 4Department of Medicine, School of Medicine, University of California Irvine, Irvine, CA, USA; 5Department of Pharmacy, City University College of Ajman, Ajman 18484, United Arab Emirates

Correspondence: Roghayeh Norouzi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Email roghayehnorouzi123@gmail.com
Mohamed Ezzat El Zowalaty
Department of Pharmacy, City University College of Ajman, Ajman 18484, United Arab Emirates
Email elzow005@gmail.com

Background: Echinococcus granulosus is causative agent of cystic echinococcosis (CE), which has a cosmopolitan distribution. The current methods for the treatment of human CE include surgery. Therefore, the development of new scolicidal agents with low side effects and more efficacies is an urgent need.
Purpose: The present study aimed to compare the scolicidal efficacies of silver, iron, copper, silica and zinc oxide nanoparticles (NPs) against hydatid cyst protoscolices in vitro.
Methods: Hydatid cysts of sheep liver and lung were collected. The cyst fluid containing protoscolices was aspirated aseptically. The scolicidal activities of the silver, iron, copper, silica and zinc nanoparticles (Ag-NP, Fe-NP, Cu-NP, Si-NP and Zn-NP) were tested at different concentrations of 0.25, 0.5 and 1 mg/mL following 10, 30 and 60 min of incubation in triplicate. Viability of protoscolices was confirmed by 0.1% eosin staining.
Results: Results showed that Ag-NPs at all concentrations tested had the highest scolicidal effect. Ag-NPs at 1 mg/mL concentration after 60 min of exposure time showed 80% mortality rate. Si-NPs had the high scolicidal activity at 1 mg/mL concentration (52.33%), Cu-NPs at 0.5 mg/mL concentration (41%), Fe-NPs at 1mg/mL concentration (28%) and Zn-NPs at concentration of 1mg/mL after 60 mins (15.67%).
Conclusion: The findings of the present study showed that Ag-NPs, Fe-NPs, Cu-NPs, Si-NPs and Zn-NPs had potent scolicidal effects and that Ag-NPs are recommended as effective scolicidal agents. However, further in vivo studies are required to evaluate the efficacy of these nanoparticles.

Keywords: scolicidal, hydatid cyst, protoscolices, silver nanoparticle, iron nanoparticle, copper nanoparticle, silica nanoparticle, in vitro

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