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Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children

Authors Selvaraju V, Venkatapoorna CMK, Babu JR, Geetha T

Received 27 February 2020

Accepted for publication 29 April 2020

Published 19 May 2020 Volume 2020:13 Pages 1695—1701

DOI https://doi.org/10.2147/DMSO.S251359

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Juei-Tang Cheng


Vaithinathan Selvaraju,1 Chandra MK Venkatapoorna,1 Jeganathan R Babu,1,2 Thangiah Geetha1,2

1Department of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USA; 2Boshell Metabolic Diseases and Diabetes Program, Auburn University, Auburn, AL, USA

Correspondence: Thangiah Geetha
Department of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, AL, USA
Tel +1 334 844 7418
Fax +1 334 844 3268
Email thangge@auburn.edu

Background: We have recently shown that the copy number of salivary amylase (AMY1) gene was significantly decreased, and the obesity-related salivary biomarkers resistin, MCP-1, TNF-α, IL-6, and CRP were significantly increased in overweight/obese children compared to normal weight. This study aimed to evaluate the association of AMY1 copy number variant (CNV) with obesity and inflammatory markers. Seventy-six participants aged between 6 and 10 years have participated, and the saliva samples were collected along with the anthropometric measurements.
Methods: AMY1 copy number was analyzed by 3D digital PCR, and obesity-related biomarkers were performed with a Bioplex multiplex analyzer.
Results: The mean AMY1 copy number was higher in normal weight (7.90 ± 0.38) compared to the overweight/obese group (6.20 ± 0.29). The association of AMY1 CNV with obesity and inflammatory markers showed significant negative correlation [CRP, β = − 0.238 (p < 0.05); resistin, β = − 0.25 (p < 0.05); MCP-1, β = − 0.304 (p < 0.01)] except for complement factor D, TNF α and IL-6. The anti-inflammatory cytokine, IL-10 reported a positive correlation with AMY1 copy number with a β = 0.268 (p < 0.05). The multivariable model adjusted with age and gender depicted a similar correlation with obesity markers.
Conclusion: Our results report that AMY1 CNV is associated with obesity and inflammatory biomarkers in children’s saliva sample.

Keywords: AMY1 copy numbers, obesity-related markers, inflammatory markers, childhood obesity, body mass index

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