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Safety and efficacy of nimotuzumab combined with chemoradiotherapy in Chinese patients with locally advanced cervical cancer

Authors Chen Y, Tang W, Pan X, Wu C, Cao Y, Yang W

Received 2 February 2017

Accepted for publication 27 April 2017

Published 17 August 2017 Volume 2017:10 Pages 4113—4119

DOI https://doi.org/10.2147/OTT.S133756

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Geoffrey Pietersz



Yong-Fa Chen, Wu-Bin Tang, Xin-Xi Pan, Chu-Rong Wu, Yang Cao, Wen Yang

Department of Oncology, People’s Hospital of Nanhai District, Southern Medical University, Foshan, People’s Republic of China


Objective:
To evaluate efficacy and safety of nimotuzumab combined with chemotherapy and radiotherapy in women with locally advanced cervical cancer.
Materials and methods: Women with locally advanced cervical cancer (stage IIB, III, or IVA) who experienced relapse after first-line chemoradiotherapy and one or more lines of palliative chemotherapy were enrolled. All patients received nimotuzumab weekly at 200 mg/m2 as single agent for 4 weeks (induction phase), then concurrent with 6 cycles (21-day per cycle) of gemcitabine (800 mg/m2) or cisplatin (50 mg/m2) for 18 weeks (concurrent phase) and then once every 2 weeks (maintenance phase). Overall response rate (ORR) was assessed after 4 weeks of induction therapy and then every 3 months according to response evaluation criteria in solid tumors version 1.1 (primary end point). Secondary end points include progression-free survival (PFS), overall survival (OS), and drug toxicity. Descriptive statistics was used for ORR, and Kaplan–Meier curves were generated for OS and PFS.
Results: A total of 80 women with locally advanced cervical cancer were enrolled and evaluated for safety and efficacy. Our results demonstrated that none of the patients had a complete response (0%), 11 patients had a partial response (14%), and 10 patients had progressive disease (13%), giving a tumor response rate of 14%. A total of 59 patients had stable disease (74%), giving a disease control rate of 88% (70/80). Median PFS was 8.21 months (95% confidence interval [CI]: 5.09–12.45). Median OS was 11.96 months (95% CI: 8.11–23.95). The most common adverse events were mucositis, myelosuppression, and gastrointestinal disturbance.
Conclusion: Our study results suggested that nimotuzumab in combination with chemotherapy and radiotherapy is well tolerated, and could be a better treatment alternative in patients with locally advanced cervical cancer.

Keywords: nimotuzumab, metastatic cervical cancer, Chinese patients, radiotherapy, chemoradiotherapy

Introduction

Cervical cancer is the second most common type of cancer for women worldwide, and it is also one of the most preventable types of cancer. It is fourth most commonly diagnosed cancer worldwide, and the fourth leading cause of cancer death in women, accounting for 9% of total new cancer cases and 7.5% of total cancer deaths in females.1,2 Cervical cancer is very common in developing countries (15% of cases), whereas in developed countries cervical cancer accounts for only 3.6%.3

Most women who are found to have cervical cancer were not screened regularly.4,5 As a result, about 25% of patients with cervical cancer present with locally advanced disease (stage IIB through IVA according to the staging system of the International Federation of Gynecology and Obstetrics).57 Among the patients with locally advanced cervical cancer, 1 out of 3 patients have treatment failure even with the most effective platinum-based doublet chemotherapy with concurrent radiation.8 Only a small proportion of patients with recurrent disease can be cured by either surgery or radiation; however, most are not, hence, systemic palliative chemotherapy is considered as the only option for patients with recurrent locally advanced cervical cancer.9

Currently, platinum-based chemotherapy (cisplatin) with taxanes (topotecan and paclitaxel) and gemcitabine is considered standard therapy because it produces similar response rates, progression-free survival (PFS), overall survival (OS) rates, and quality-of-life outcomes.10 A study evaluated the efficacy of bevacizumab in combination with chemotherapy in advanced cervical cancer; the results of this study showed that the addition of bevacizumab to chemotherapy regimen significantly improve PFS and OS.11 However, these results were not consistent with phase II clinical study in which bevacizumab was combined with the cisplatin–topotecan therapy in patients with recurrent or persistent cervical cancer.11

Overexpression of epidermal growth factor receptor (EGFR) in cervical cancer is well documented (overexpression ranges from 6% to 90%); this makes EGFR as a potential target in cervical cancer.12 Nimotuzumab is a humanized IgG1 monoclonal antibody which blocks the activation of EGFR without intrinsic stimulating activity. Nimotuzumab displayed a longer plasma half-life with better safety profile when compared with other anti-EGFR antibodies.13,14 Nimotuzumab is approved in some countries for the treatment of glioma and advanced head and neck cancer as a single agent or in combination with chemotherapy and radiotherapy.12 Efficacy and safety of nimotuzumab as a single agent or in combination with chemotherapy and radiation have been evaluated in cervical cancer; however, no results have been published. A pilot study was conducted to evaluate efficacy and safety of nimotuzumab in combination with chemotherapy among Caucasian patients with advanced or progressive cervical cancer.12 The results of this study showed that nimotuzumab is well tolerated and may have a role in the treatment of advanced cervical cancer. However, to date, there has been no study that evaluates the efficacy and safety of nimotuzumab in combination with chemoradiotherapy in Chinese patients with locally advanced cervical cancer. We, therefore, designed pilot, phase IIa, prospective, single-arm study to evaluate efficacy and safety of nimotuzumab in combination with chemoradiotherapy in Chinese patients with locally advanced cervical cancer. Our study will serve as the basis for conducting large multi-centric randomized clinical study among Chinese patients with locally advanced cervical cancer.

Methods

Patients

In this pilot, phase IIa, prospective, open-label, single-arm study, women with locally advanced cervical cancer who experienced disease relapse after receiving first-line chemoradiotherapy and one or more lines of palliative chemotherapy were enrolled in the study from January 2010 to January 2015. The patients were enrolled at Department of Oncology, People’s Hospital of Nanhai District, Southern Medical University, People’s Republic of China. All patients provided written informed consent before screening and other study-related procedures, and the study was approved by the ethics committee of the Southern Medical University.

Women with histologically and/or radiologic confirmed diagnosis of squamous-cell carcinoma, adenosquamous carcinoma or adenocarcinoma of the cervix of International Federation of Gynecology and Obstetrics stage IIB (localized disease with parametrial involvement), stage III (extension of the tumor to the pelvic wall) or stage IVA (involvement of the bladder or rectal mucosa) were enrolled. All patients had measurable disease and were required to have an Eastern Cooperative Oncology Group (ECOG) score of 0–2, and at least 1 prior systemic chemotherapy regimen for metastatic recurrent disease administered at least 3 weeks before starting protocol therapy. Other eligibility criteria were as follows: absolute neutrophil count ≥1.5×109/L, platelets count ≥100×109/L, total bilirubin, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≤1.5× upper limit of normal and calculated creatinine clearance (Cockroft) ≥50 mL/min. The patients with uncontrolled systemic disease or infection, pregnancy or breastfeeding, and previous or concurrent malignancy other than adequately treated nonmelanoma skin cancer were excluded.

Treatment

Antibody and chemotherapy

All the enrolled patients received intravenous infusion of nimotuzumab (monoclonal antibody) alone at a dose of 200 mg/m2 for 30 minutes weekly during the first 4 weeks (induction phase) followed by 18 weekly applications of nimotuzumab concurrently with 6–21 days cycles of single-agent chemotherapy (concurrent). After week 18 (22 weeks since treatment initiation), patients continued with nimotuzumab alone (maintenance) once every 2 weeks at the same dose until unacceptable toxicity or consent withdrawal. No premedication including rash prophylaxis was administered before nimotuzumab infusion. Single-agent chemotherapy comprised intravenous infusion of gemcitabine (800 mg/m2) for 30 minutes, or cisplatin (50 mg/m2) was administered intravenously in an outpatient setting.

Radiotherapy

All the enrolled patients received radiotherapy. Radiotherapy was administered to the whole pelvic region in 24 fractions totaling 40.8 Gy or 30 fractions totaling 51.0 Gy. The total dose delivered was 40 Gy in patients with stage IIB, and 30 Gy in patients with stage III or IVA disease. The total dose delivered to point A (a reference location 2 cm lateral and 2 cm superior to the cervical OS) was 80.8 Gy in patients with stage IIB and 81.0 Gy in patients with stage III or IVA disease; the total dose delivered to point B (the pelvic wall) was 55.0 Gy in patients with stage IIB disease and 60.0 Gy in patients with stage III or IVA disease. Pelvic radiation was delivered by a four-field box technique (anteroposterior, posteroanterior and two lateral fields) with an X-ray energy of at least 4-mV photons. The pelvic field extended from the upper margin of L5 to the midportion of the obturator foramen or the lowest level of disease, with a 3-cm margin, and laterally 1.5–2 cm beyond the lateral margins of the bony pelvic wall (at least 7 cm from the midline). The duration of the radiotherapy was 10 weeks. Radiotherapy was withheld if a patient had a leukocyte count of <2,000/mm3, and delays of up to 1 week were also allowed in the event of radiation-related gastrointestinal or genitourinary toxicity.5

Efficacy and safety assessment

Each patient was required to undergo a complete physical examination, a pelvic examination under anesthesia, chest radiography and intravenous pyelography or abdominal computed tomography to determine the clinical stage of the cancer. Baseline examination included a complete history and physical examination, complete blood cell count, blood chemistry and imaging by computed tomography (CT) or magnetic resonance imaging (MRI) of target lesions (4 weeks before starting the study). Primary end point was overall response rate. Overall response rate was defined as the proportion of enrolled patients who qualify for the analysis population of interest and who have a best response of partial response (PR) or complete response (CR) according to the response evaluation criteria in solid tumors (RECIST) 1.1 guidelines. Overall response rate was evaluated from patient’s inclusion after 4 weeks of induction therapy and then every 3 months until second year of follow-up for each patient. Treatment response was evaluated according to the RECIST criteria (1.1) by the same imaging modality used for baseline evaluation. Disease control rate was defined as the proportion of enrolled patients, qualified for the analysis population of interest, having a best response of PR, CR or stable disease (SD) according to RECIST 1.1 guidelines. Secondary end points include PFS, OS, and drug toxicity. PFS was defined as the time elapsed since the beginning of interventions until progressive disease is documented by growth of measurable lesions or new lesions on CT scan or MRI according to the response evaluation criteria in solid tumors (time frame: after patient’s inclusion every 3 months for a period 2 years). OS was defined as the time from date of enrollment until date of death due to any cause (time frame: from patient’s inclusion until 24 months). Drug toxicity was assessed with hematologic, renal, and hepatic laboratory measures as well as patient symptomatology and physical findings. Any abnormality in these parameters was reported as an adverse event according to the Common Terminology Criteria for Adverse Events version 3.0 (CTCAE v3.0). Adverse events were recorded according to CTCAE v3.0 at each visit before treatment administration. Follow-up assessments were made 4 weeks after the last dose of study medication and every 3 months thereafter.

Statistical analysis

A sample size of 75 evaluable patients with histologically confirmed diagnosis of locally advanced cervical cancer was sufficient to detect an assumed clinically significant difference of 11% in response rate after treatment with nimotuzumab combined with radiotherapy and chemotherapy, using a two-tailed test with 80% power and 5% level of significance. Considering dropout rate of 5%, adjusted sample size was ~80 patients of histologically confirmed diagnosis of locally advanced cervical cancer. Hence, a total of 80 patients with histologically confirmed diagnosis of locally advanced cervical cancer required to enroll in this study to complete 75 evaluable patients, to evaluate efficacy and safety of nimotuzumab in combination with chemoradiotherapy in Chinese patients with locally advanced cervical cancer. Patients who had a presence of measurable disease and had at least 1 baseline and 1 postbaseline tumor measurement were considered to be tumor response-qualified population. Overall response rate and disease control rate end points were presented using descriptive statistics for tumor response-qualified patients. Kaplan–Meier curves were generated for OS and PFS for enrolled population (all patients who met eligibility criteria and enrolled). Median survival time together with 95% confidence interval (CI) was calculated. Data were analyzed using Graph Pad Prism statistical analysis software (version 6.0).

Results

Patient characteristics

Between January 2010 and January 2015, a total of 80 women with locally advanced cervical cancer were enrolled and treated with nimotuzumab combined with chemotherapy and radiotherapy. Data from all 80 patients were subjected to statistical analysis. The key clinicopathological characteristics are shown in Table 1. The median age was 59 years (range 24–78), most were squamous cell carcinomas and had an ECOG performance status of 1. The majority of patients had stage III and stage IVA of cervical cancer.

Table 1 Patients’ characteristics

Efficacy

Of total 80 patients with locally advanced cervical cancer, no patient had a CR (0%), 11 patients had a PR (14%), and 10 patients had progressive disease (13%), giving an overall response rate of 14% (Table 2). A total of 59 patients had SD (74%), giving a disease control rate of 88% (70/80). Median PFS was 8.21 months (95% CI: 5.09–12.45). Median OS was 11.96 months (95% CI: 8.11–23.95). The Kaplan–Meier curve of PFS (enrolled population) and OS (enrolled population) is presented in Figures 1 and 2, respectively.

Table 2 Summary of tumor response (tumor-response qualified population)

Figure 1 Kaplan–Meier curve of PFS (enrolled population).
Abbreviations: CI, confidence interval; PFS, progression-free survival.

Figure 2 Kaplan–Meier curve of OS (enrolled population).
Abbreviations: CI, confidence interval; OS, overall survival.

Safety

The types and frequencies of treatment-related adverse events experienced in women with locally advanced cervical cancer after nimotuzumab plus chemoradiotherapy are shown in Table 3. The most common adverse events after nimotuzumab plus chemoradiotherapy were mucositis, myelosuppression, and gastrointestinal disturbance; however, no grade 4 events were registered. The grade 3 toxicity events observed were mucositis (10%), gastrointestinal disturbance (3%), leukocytopenia (4%), anemia (4%), thrombocytopenia (5%), and radiodermatitis (1%). The most frequent adverse events of all grades reported (occurs in ≥20% of patients) after nimotuzumab plus chemoradiotherapy were mucositis (45 [56%] cases), febrile neutropenia (2 [5.4%] cases), gastrointestinal disturbance (28 [35%] cases), leukocytopenia (37 [46%] cases), anemia (27 [34%] cases), thrombocytopenia (35 [44%] cases), granulocytopenia (22 [28%] cases), and febrile neutropenia (20 [25%] cases). Other less common adverse events of all grades reported after nimotuzumab plus chemoradiotherapy were (occurs in ≥15% of patients) nausea/vomiting (19%), radiodermatitis (19%), weight loss (16%), anorexia (16%), rash (16%), and fatigue (15%). There were no treatment-related deaths. No patient was discontinued from study and study drug due to adverse events. Overall, the safety data suggest that nimotuzumab combined with radiotherapy and chemotherapy has an acceptable safety profile in Chinese women with locally advanced cervical cancer.

Table 3 Summary of treatment-related adverse events experienced in women with locally advanced cervical cancer after nimotuzumab plus chemoradiotherapy
Note: Grade 1 indicates minimal effect, grade 2 indicates mild effect, grade 3 indicates moderate effect, and grade 4 indicates severe effect.

Discussion

We present the first evidence that nimotuzumab combined with concurrent chemoradiotherapy in the treatment locally advanced cervical cancer. The results of this study demonstrate that nimotuzumab in combination with chemoradiotherapy is well tolerated and yields overall response rate of 14%, disease control rate of 88% and disease stabilization in 74% of the patients. We have not observed CR in any of enrolled patients, this possibly due to the women of advanced cervical cancer were only enrolled. The observed effect translates into PFS (95% CI) and OS (95% CI) rates of 8.21 months (5.09–12.45) and 11.96 months (8.11–23.95), respectively.

EGFR is one of the key therapeutic targets for cancer treatment as it plays an important role in tumorigenesis and maintenance of cancers. Overexpression of EGFR is well documented in various types of malignancies including head and neck, colon, breast cancers and cervical cancer.15 In this setting, several EGFR inhibitors have been tested, including sunitinib, imatinib and temsirolimus but no evidence of any clinical benefit was observed.13,14 Among other monoclonal antibodies against the EGFR, only cetuximab has been evaluated in cervical cancer. The effect of cetuximab was tested in 5 patients with cervical cancer, only 1 patient reported with SD.16 In another clinical study, no clinical benefit was observed after treatment with cetuximab.17 Among EGFR inhibitors, nimotuzumab has demonstrated efficacy characterized by the induction of a long-term SD with a very low toxicity in patients with head and neck squamous cell carcinoma, pancreatic cancer, lung cancer and brain tumors.12,15 Nimotuzumab has already been approved for the treatment of advanced head and neck, glioma and esophageal cancer patients in combination with irradiation or chemoradiotherapy.12,15

The results of phase II study have suggested that nimotuzumab in combination with radiotherapy is well tolerated in locally advanced rectal cancer.18 Another pilot trial aimed to evaluate the efficacy and safety of nimotuzumab in Caucasian patients with advanced cervical cancer concluded that nimotuzumab is well tolerated and may have a role in the treatment of advanced cervical cancer.12 Our study results are in line with the previous study,12 however, the diseases’ stabilization rate was better (74%) when compared to Caucasian patients (35%) who received nimotuzumab in combination with chemotherapy only.12 Moreover, in our study, the overall response rate and disease control rate after treatment of nimotuzumab in combination with chemotherapy and radiotherapy was 14% and 88%, respectively. This indicates that the combination of nimotuzumab with chemotherapy and radiotherapy could be a better treatment option in patients with locally advanced cervical cancer.

Conclusion

Our study results suggested that nimotuzumab in combination with chemotherapy and radiotherapy is well tolerated, and could be a better treatment alternative in patients with locally advanced cervical cancer. Nimotuzumab combined with concurrent chemoradiotherapy has potential role in the treatment of locally advanced cervical cancer.

Acknowledgments

This work was supported by Foshan Medical Science and Technology Projects (No 2014AB000302). The authors thank all patients for their participation in the study. We would also like to thank Rakesh Ojha (senior consultant medical writer) for writing and editorial support in the preparation of this manuscript.

Disclosure

The authors report no conflicts of interest in this work.


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