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ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles

Authors Piktel E, Ościłowska I, Suprewicz Ł, Depciuch J, Marcińczyk N, Chabielska E, Wolak P, Wollny T, Janion M, Parlinska-Wojtan M, Bucki R

Received 19 August 2020

Accepted for publication 8 December 2020

Published 9 March 2021 Volume 2021:16 Pages 1993—2011


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Farooq A. Shiekh

Ewelina Piktel,1 Ilona Ościłowska,2 Łukasz Suprewicz,1 Joanna Depciuch,3 Natalia Marcińczyk,4 Ewa Chabielska,4 Przemysław Wolak,5 Tomasz Wollny,6 Marianna Janion,5 Magdalena Parlinska-Wojtan,3 Robert Bucki1

1Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, 15-222, Poland; 2Department of Medicinal Chemistry, Medical University of Bialystok, Bialystok, 15-222, Poland; 3Institute of Nuclear Physics Polish Academy of Sciences, Krakow, PL-31342, Poland; 4Department of Biopharmacy, Medical University of Bialystok, Bialystok, 15-222, Poland; 5 Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University, Kielce, 25-317, Poland; 6Holy Cross Cancer Center in Kielce, Kielce, 25-734, Poland

Correspondence: Robert Bucki
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2C, Bialystok, 15-222, Poland
Tel + 48 85 748 5793
Email [email protected]

Background: Even with considerable improvement in treatment of epithelial ovarian cancer achieved in recent years, an increasing chemotherapy resistance and disease 5-year relapse is recorded for a majority part of patients that encourages the search for better therapeutic options. Gold nanoparticles (Au NPs) due to plethora of unique physiochemical features are thoroughly tested as drug delivery, radiosensitizers, as well as photothermal and photodynamic therapy agents. Importantly, due to highly controlled synthesis, it is possible to obtain nanomaterials with directed size and shape.
Methods: In this work, we developed novel elongated-type gold nanoparticles in the shape of nanopeanuts (AuP NPs) and investigated their cytotoxic potential against ovarian cancer cells SKOV-3 using colorimetric and fluorimetric methods, Western blot, flow cytometry, and fluorescence microscopy.
Results: Peanut-shaped gold nanoparticles showed high anti-cancer activity in vitro against SKOV-3 cells at doses of 1– 5 ng/mL upon 72 hours treatment. We demonstrate that AuP NPs decrease the viability and proliferation capability of ovarian cancer cells by triggering cell apoptosis and autophagy, as evidenced by flow cytometry and Western blot analyses. The overproduction of reactive oxygen species (ROS) was noted to be a critical mediator of AuP NPs-mediated cell death.
Conclusion: These data indicate that gold nanopeanuts might be developed as nanotherapeutics against ovarian cancer.

Keywords: gold nanoparticles, ovarian cancer, gold nanopeanuts, anti-cancer therapy, nanotechnology, apoptosis, autophagy

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