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Roles of microRNA-99 family in human glioma

Authors Zhang M, Guo Y, Wu J, Chen F, Dai Z, Fan S, Li P, Song T

Received 28 October 2015

Accepted for publication 28 January 2016

Published 20 June 2016 Volume 2016:9 Pages 3613—3619

DOI https://doi.org/10.2147/OTT.S99363

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Ram Prasad

Peer reviewer comments 2

Editor who approved publication: Professor Min Li


Mingyu Zhang,1 Yong Guo,1 Jun Wu,1 Fenghua Chen,1 Zhijie Dai,2 Shuangshi Fan,1 Pengcheng Li,1 Tao Song1

1Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 2Institute of Endocrinology and Metabolism, Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of China

Objective: Deregulation of microRNA (miR)-99 family members (miR-99a, miR-99b, and miR-100) has been reported to play a crucial role in many cancer types. However, their roles in human gliomas have not been fully elucidated. This study aimed to investigate the expression patterns of miR-99a, miR-99b, and miR-100 in glioma tissues and to evaluate their expression profiles with respect to tumor progression.
Methods: Quantitative real-time polymerase chain reaction was performed to detect the expression levels of miR-99a, miR-99b, and miR-100 in glioma and matched non-neoplastic brain tissues. Then, the associations of their expression with various clinicopathological features of glioma patients were statistically analyzed. Moreover, the roles of miR-99a, miR-99b, and miR-100 in regulating glioma cell migration and invasion were determined via transwell assay in vitro.
Results: Compared with non-neoplastic brain tissues, miR-99a, miR-99b, and miR-100 expression levels were all significantly decreased in glioma tissues (all P<0.001). miR-99a-low, miR-99b-low, and miR-100-low expression more frequently occurred in glioma patients with low Karnofsky performance score (<90) and high World Health Organization grade (III–IV). Further functional experiments revealed that the enforced expression of miR-99a, miR-99b, and miR-100 resulted in the inhibition of cellular migration and invasion in glioma cells.
Conclusion:
Our results strongly suggest that the aberrant expression of miR-99a, miR-99b, and miR-100 may be a common feature in human gliomas with aggressive clinicopathological features and may participate in malignant phenotypes of the tumors. These findings highlight the potential of the three miR-99 family members as novel therapeutic targets for human gliomas.

Keywords: microRNA-99 family, glioma, clinicopathological features, migration, invasion

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