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Role of lncRNA NR2F1-AS1 and lncRNA H19 Genes in Hepatocellular Carcinoma and Their Effects on Biological Function of Huh-7

Authors Ji WC, Bao GJ, Yang FW, Sun L, Han R

Received 20 October 2020

Accepted for publication 26 December 2020

Published 3 February 2021 Volume 2021:13 Pages 941—951

DOI https://doi.org/10.2147/CMAR.S284650

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Ahmet Emre Eşkazan


Wen-Chao Ji,1 Guang-Jian Bao,1 Fang-Wu Yang,2 Lei Sun,1 Rui Han1

1Department of Hepatobiliary Surgery, Zaozhuang Municipal Hospital, Zaozhuang, 277100 Shandong Province, People’s Republic of China; 2General Surgery Department, Zaozhuang Mining Group Central Hospital, Zaozhuang, 277100 Shandong Province, People’s Republic of China

Correspondence: Rui Han
Department of Hepatobiliary Surgery, Zaozhuang Municipal Hospital, NO. 41 Longtou Road, Shizhong District, Zaozhuang, 277100, Shandong Province, People’s Republic of China
Tel + 86-18369221166
Email hanruihenry@126.com

Objective: This research was designed to probe into the expression and related mechanism of lncRNA NR2F1-AS1 and H19 in hepatocellular carcinoma (HCC).
Methods: Forty-two HCC patients who came to our hospital from February 2018 to August 2019 were included into a research group (RG). Meanwhile, 46 healthy controls were regarded as a control group (CG). BEL-7402, Huh-7 human hepatoma cells and HL-7702 human normal liver cells were purchased, and the NR2F1-AS1 and H19 levels in serum and tissues of HCC patients were detected. PcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19 and si-H19 were transfected into BEL-7402 and Huh-7 cells. The NR2F1-AS1 and H19 levels in samples were detected via qRT-PCR, and the expression of apoptosis-related proteins in cells was tested through WB. Cell proliferation, invasion, or apoptosis was detected by CCK8, Transwell or flow cytometry, respectively.
Results: The NR2F1-AS1 and H19 levels were high in human hepatoma cells, and AUCs of lncRNA NR2F1-AS1 and lncRNA H19 were both > 0.8. The lncRNA NR2F1-AS1 and lncRNA H19 were associated with HCC staging. After transfection of pcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19, si-H19 BEL-7402 and Huh-7 cells, silencing NR2F1-AS1 and H19 expression can promote apoptosis and inhibit cell growth, while silencing their over-expression can inhibit the EMT process of Huh-7 cells.
Conclusion: lncRNA NR2F1-AS1 and lncRNA H19 genes are abnormally expressed in HCC. Furthermore, the two can suppress the EMT process of Huh-7 cells and promote apoptosis effectively.

Keywords: lncRNA H19, lncRNA NR2F1-AS1, hepatocellular carcinoma, HCC, trophoblast cells, invasion, apoptosis

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