Role of Goblet Cells in Intestinal Barrier and Mucosal Immunity

Introduction

The intestinal tract is essential in controlling nutrient digestion and absorption while functioning as a barrier to prevent foreign antigens and pathogens from entering the mucosal tissues and maintaining intestinal homeostasis. The intestinal barrier system depends on interactions among several barrier components, including mucus layer, epithelial layer and intercellular tight junctions and the lamina propria underneath.^1,2 Among these components, the integrity of the mucus barrier formed by goblet cells and their secretions play a vital role in maintaining intestinal homeostasis. Goblet cells secrete mucins, which are high-molecular-weight glycoproteins, denoting the primary structural element of the mucus layer.^3,4 The mucins are highly hydrophilic and can bind water to form a gel-like structure, preventing direct contact between enterocytes and the intraluminal content, especially pathogenic microorganisms.⁴ The absence of or any defect in the mucus layer allows a large number of bacteria to make contact with the epithelial cells, triggering an excessive immune response in the host,⁵ leading to colitis in mice.⁶ Various intestinal infections resulting from parasites,^7,8 viruses,⁹ and bacteria¹⁰ modify the production of mucin and goblet cells, demonstrating the importance of the mucus layer in separating the luminal contents from the epithelium. However, the critical function of the mucus layer and goblet cells in host defense has not received adequate attention. Recent studies have started to focus on the ability of goblet cells to actively sense and respond to infections while secreting additional products, such as trefoil factor peptides (TFF), mucins, Fc-γ binding protein (Fcgbp), and resistin-like molecule β (RELMβ), which are crucial in promoting intestinal defense.^11–13 This review summarizes the current advancements regarding the signaling pathways that control goblet cell differentiation while discussing the new functional responsibility of goblet cells in intestinal mucosal immunology.

The Differentiation of Goblet Cells

The self-renewal of intestinal epithelial cells is maintained by the proliferative activity of adult stem cells located at the base of the intestinal crypts. The progeny of these stem cells proliferates and then differentiates into functional epithelial subtypes that migrate to the villi and eventually into the lumen shed into the gut lumen.^14,15 The four main types, namely Paneth cells, intestinal epithelial cells, enteroendocrine cells, and goblet cells, are derived from the stem cells in the basement of the crypt.¹¹ Enterocytes represent the majority (up to 80%) of cells in the intestinal epithelium, they are responsible for ion, water, sugar, peptide and lipid uptake.¹⁵ The basal part of endocrine cells contains a large number of dense neuroendocrine granules, which contain the secreted peptide hormones, secreted basally in an endocrine or paracrine manner.¹⁶ The goblet cell contains mucigen granules that are expelled to the surface as intestinal mucus, protecting and lubricating the mucosa. Paneth cells are primarily located in the small intestine, where they secrete a number of mediators of host defense, including lysozyme, tumor necrosis factor, and defensins, that protect against intestinal bacterial pathogens.¹⁷ Dynamic analysis of the goblet cells in the intestines of mice indicated a migration from the crypt base to the villi tip once after differentiation, where they enter the lumen.^18,19 The upper crypt cells of the colon show that during differentiation, the goblet cells develop the capacity to generate and store significant quantities of mucus.⁵ These immature goblet cells are located at the base of the crypt in a pyramidal shape.¹⁸ The goblet cell cannot be distinguished morphologically in Mucin2 (Muc2) deficient mice, despite the continued presence of other goblet cell products, such as TFF.²⁰ Maintaining stem cells and the distinction into four main types of intestinal cell lineages involves a variety of complex signaling pathways, such as Wnt/β-catenin, Notch, PI3-kinase/Akt, and bone morphogenetic protein (BMP) signaling.²¹ The canonical Wnt pathway is tightly linked with cell proliferation, differentiation and stem cell maintenance.^15,22 Wnt ligands bind to the Frizzled–LRP5–LRP6 receptor complex, which inhibits continuous destruction of β-catenin by the cytoplasmic adenomatous polyposis coli (APC) destruction complex in the intestinal epithelium.²³ The accumulation of β-catenin leads to its translocation to the nucleus, where it binds T cell factors (TCFs) and directly regulates gene expression.²³ Using transgenic mice ectopically expressing Dickkopf1 (Dkk1), a secreted Wnt inhibitor, Pinto et al²⁴ found that epithelial proliferation is highly reduced simultaneously with the loss of crypts. Although enterocyte differentiation appeared unaffected, secretory cell lineages were largely absent. In the presence of WNT, stabilized β-catenin can bind the Hes1 promoter together with Notch intracellular domain (NICD), resulting in stable Notch activation and promoting the initial absorptive or secretory cell differentiation decision by lateral inhibition.^25,26 Higher up in the crypt, in the absence of WNT, negative feedback of the Hes1 promoter and absence of nuclear β-catenin causes oscillatory Notch activation and enables stochastic secondary fate decisions within a lineage (for example goblet versus enteroendocrine cell fate²⁶). The Notch signaling pathway significantly regulates intestinal enterocyte lineage, activating the hairy and enhancer of split 1 (Hes1) transcription factor, repressing the basic helix-loop-helix (bHLH) transcription factor mouse atonal homolog 1 (Math1),^11,27 also known as Atonal homologue 1 (Atoh1). The Notch-Hes1 pathway promotes intestinal progenitor cell differentiation toward luminal epithelial cells, restricting the development of secretory cells. Notch signaling pathway activation disrupts the differentiation of secretory cells with the villi coated primarily with absorptive enterocytes associated with Hes1 activation.^11,21 Math1 facilitates the distinction of intestinal stem cells into the goblet cell lineage and is seemingly essential for differentiating intestinal secretory lineages since studies have shown that Math1-deficient mice failed to generate three gastrointestinal mucosal cell types, namely enteroendocrine, Paneth, and goblet cells.^18,28 As indicated by previous research, the transcriptional activation of the Jagged1 Notch-ligand, mediated by β-catenin, leads to Notch being downstream of Wnt in colorectal cancer cells.^29,30 Furthermore, the terminal differentiation of goblet cells involves the activation of Krüppel-like transcription factor 4 (Klf4), SAM pointed domain-containing ETS transcription factor (Spdef), and growth factor independence 1 (Gfi1). As a downstream target of Math1, Gfi1 controls intestinal secretory cell subtype allocation and differentiation.³¹ Furthermore, fewer supernumerary enteroendocrine and goblet cells were evident in Gfi1 knockout mice, while they lacked Paneth cells. Gfi1-null crypts containing no Paneth cells and only a few goblet cells display a quantitative reduction in Spdef, which is absent from Atoh1-null crypts lacking intestinal secretory cells, suggesting Spdef functionality downstream of Gfi1 and Math1 in the goblet cell terminal differentiation pathway³² (Figure 1). In the intestine, Klf4 regulates goblet cell terminal differentiation by controlling Muc2 expression,³³ which can be inhibited by the Notch signaling pathway.³⁴ Recent studies indicate that prolyl hydroxylase 3 (PHD3) also controls the generation of intestine goblet cell by bounding the E3 ubiquitin ligase HUWE1 and inhibition of HUWE1-mediated ubiquitination and degradation of ATOH1.³⁵

Figure 1 Role of transcription factors in goblet cell differentiation.

Various additional factors, such as immune cells, diet, and bacteria, also influence goblet cell differentiation.^36–38 Significantly fewer intestinal goblet cells in germ-free mice only express modest levels of MUC2 while containing an exceedingly thin mucus layer compared with conventionally housed mice.³⁹ However, exposing germ-free mice to a conventional environment enhances RELMβ and MUC2 expression, leading to a substantially thicker mucus layer.⁴⁰ The microbiota, acting via secreted factors related to indole, promote goblet cell differentiation and regulate intestinal homeostasis via the xenobiotic aryl hydrocarbon receptor to increase expression of the cytokine interleukin-10 (IL-10), reversing an effect of aging in geriatric mice.⁴¹ Therefore, these results support the vital role of microbial colonization in goblet cell development and maturation.

The Classification and Structure of Mucins

Mucins consist of large glycoproteins containing tandem repeats with high levels of serine and threonine, with the hydroxyl residues displaying a significant number of O-linked oligosaccharides.⁴ To date, 21 different mucin genes have been detected, designated MUC1 to MUC21 according to the order of their discovery.⁴² Furthermore, based on their structural characteristics and biological functionality, mucins are segregated into two primary groups, namely membrane-associated mucins and secreted mucins. Intestinal membrane-associated mucins are denoted by MUC1, MUC3A/B, MUC4, MUC12, MUC13, MUC15, MUC17, MUC20, and MUC21. Secreted mucins can be divided into gel-forming mucins (MUC2, MUC5AC, MUC5B, MUC6, and MUC19), which are essential during the development of the mucus barrier on mucosal surfaces, and non-gel forming mucins (MUC7, MUC8, and MUC9).^42–46 Gel-forming mucins play a functional protective, transportation, lubrication, and hydration role in the mucous membranes.⁴⁷ Minimal information is available regarding the functionality of non-gel forming mucins. Membrane mucins provide a safe epithelial cell barrier while playing an important role in signal transduction.⁴³ A summary of the mucin classification is listed in Table 1.

Table 1 The Classification of Mucins

Regulation of Mucin Synthesis

The endoplasmic reticulum (ER) represents the organelle where mucins are synthesized, and N-linked glycosylation occurs. The assembled mucins are transported to the Golgi complex, where they are O-linked glycosylated to a size of 2.5 million Daltons.^48–50 They are then packaged as secretory granules, accounting for about 75% of the cytoplasmic volume.⁵¹ The granules mature to produce highly concentrated mucins that eventually merge with the plasma membrane and are secreted into the extracellular domain.⁵² The mucin structure changes to form a gelatinous combination with water, covering the surface of the epithelium.⁴⁹ MUC2 denotes the prominent intestinal mucin secreted by healthy mice, the deficiency of which leads to spontaneous inflammation and infection susceptibility.⁶

Recent studies have revealed that epigenetic and transcriptional regulation primarily controls the expression of mucin.^43,47,53 Signaling pathways control Muc2 transcriptional regulation, activating the transcription factors binding to specific Muc2 promoter sites. Negative or positive Muc2 transcription is reportedly regulated by several biologically active molecules, including growth factors, hormones, microbial products, and cytokines.⁴³ Muc2 gene expression regulation is essentially governed by the promoter region. The promoter structures of Muc2 reveal that a typical TATA box exists at the 31/−25 bp upstream location of the transcriptional initiation site.^54,55 The Muc2 gene 5′-flanking areas display a CACCC box that specifically binds to the specificity protein 1 (Sp1) transcription factor. In addition, transcription factor p53 can activate the transcription of Muc2 by binding to both the −1131 /−1100 and −676 /−650 sites.⁵⁶ Moreover, NF-κB is also associated with the upregulation of Muc2 transcription,⁵⁷ representing the final effector molecule that regulates Muc2 expression in multiple signaling pathways (Figure 2).

Figure 2 Schematic representation of the promoter regions of MUC2.

Many other factors impact the expression of Muc2 by directly binding on different sites of the promoter, including short-chain fatty acids (SCFAs),⁵⁸ galectin-3,⁵⁹ homeobox domains (Cdx),⁶⁰ the GATA family,⁶¹ and HATH1.⁶² SCFAs are metabolites formed by gut microbiota from dietary fiber, including acetate, propionate, and butyrate.⁶³ As part of the β-galactoside-binding gene family, galectin-3 is implicated in tumor progression, cell migration, adhesion, and apoptosis.^59,64 Both butyrate and galectin-3 stimulate Muc2 expression through the AP-1 transcription factor binding site in the Muc2 promoter.^58,65 AP-1 is a dimeric protein complex that consists of c-Jun and c-Fos proto-oncogenes, the expression of which can be facilitated by butyrate.⁵⁸ Two Cdx-2 binding sites are present in the Muc2 promoter at −177/-171 and −191/-187, suggesting that Cdx-2 is a transcriptional regulator for Muc2.⁶⁰ GATA exists in the Muc2 gene 5′-flanking region and comprises six transcription factors in the highly conserved zinc finger DNA-binding domain, which is responsible for upregulating Muc2 gene expression.^61,66 HATH1 and MATH1 are bHLH transcription factors essential in regulating the differentiation of goblet cells.^18,28 HATH1 binding sites are present in the Muc2 promoter sequence, the mutation of which down-regulates the expression of Muc2.⁶²

Some bacterial products regulate the production of Muc2 indirectly by activating the NF-kB pathway, including lipopolysaccharides (LPS), Gram-negative bacterial flagellin A, and Gram-positive bacterial lipoteichoic acid (LTA). Muc2 transcription is upregulated by Gram-negative Pseudomonas aeruginosa LPS by activating NF-κB via the Ras-mitogen-activated protein kinase (MAPK) pathway in the intestinal epithelial cells.^57,67 However, flagellin binds to the Asialo-GM1 glycolipid receptor on the surface, releasing ATP and subsequently binding to the cell surface G protein-coupled receptor (GPCR). This increases the intracellular calcium levels, activating NF-κB via the downstream signaling pathways.⁵⁷ Furthermore, LTA binds and activates the platelet-activating factor receptor, a cell surface GPCR, inactivating the epidermal growth factor receptor (EGFR), in turn leading to the activation of the Ras/Raf/MEK/ERK/pp90rsk/NF-kB pathway while upregulating the transcription⁶⁸ of Muc2.

Furthermore, several cytokines and chemokines are involved in mucin synthesis. The Th1 type cytokine, tumor necrosis factor-α (TNF-α), upregulates the transcription of Muc2 via the PI3K/AKT/NF-κB signaling pathway. Moreover, TNF-α also inhibits the transcription of Muc2 through the JNK pathway, but overall effect of is a net increase in Muc2 transcription, because NF-κB transcriptional activation of this gene is able to counter-balance the suppressive effects of the JNK pathway.⁶⁹ However, TNF-α inhibited Muc2 production when NF-κB was inactivated, which gives rise to the defective mucosal protection.⁶⁹ Vasoactive intestinal peptide (VIP), a neuropeptide hormone, is responsible for the Muc2 transcription upregulation by activating the CREB/ATF1 transcription factors via the p38 and MAPK pathways.⁷⁰ PGE2 also induced Muc2 transcription by activation of CREB/ATF1. The underlying molecular mechanisms of another Th1 type cytokine, IL-1β, induces the Muc2 activation of the p38 and ERK pathways, leading to cyclooxygenase 2 expression, an enzyme related to PGE2 synthesis.⁷¹ Moreover, IL-4 and IL-13 are Th2 cytokines that can upregulate the expression of Muc2 through the MAPK/NF-κB mediated pathway.⁷²

Epigenetic regulation includes microRNA silencing, histone modification, and DNA methylation. The methylation of specific CpG sites in the promoter region and first intron of Muc2 is associated with the repression of MUC2.⁷³ Recent studies have revealed that the expression of Muc2 gene is controlled by the methylation of DNA and the modification of histone in the 5′ flanking area of the Muc2 promoter.⁷⁴

Regulation of Mucin Secretion

After mucin proteins are synthesized in the goblet cells, they are tightly packed into intracytoplasmic granules. They are then transported to the surface of the cell and ultimately secreted into the lumen. Mucin secretion can be divided into two types, namely constitutive and stimulated secretion. In typical physiological conditions, goblet cells are synthesized continuously, secreting mucins to form the hydrated gel coating on the intestinal mucosal luminal surface. This continual secretion of mucin is essential for maintaining the thickness of the mucus gel. It is constantly subjected to various microbial pathogens and stimuli and is often shed due to peristaltic intestinal movements.⁷⁵ The release of mucin is accelerated when goblet cells are subjected to powerful secretagogues and is influenced by many different factors, including neuropeptides, cytokines, and lipids.⁷⁶ Bioactive cytokine binds to specific receptor-affecting secondary messengers and signaling components, such as intracellular diacylglycerol, cAMP, and Ca2+, activating protein kinase C to promote the secretion^67,77 of mucin. The prostaglandin E2 (PGE2) immune modulator binds the EP4 receptor, promoting cAMP-dependent exocytosis in the human colon.^78,79 Carbachol, a Ca2+-mediated agonist, elevates the cytosol levels of Ca2+, which stimulates the secretion⁸⁰ of mucin. Phorbol 12-myristate 13-acetate (PMA) significantly promotes the release of mucin via the protein-kinase C-dependent pathway.⁷⁷

Recent research has indicated that the mucus secretion of goblet cells is modulated by several cellular processes, including the assembly and activation of inflammasomes, the generation of reactive oxygen species (ROS),^4,5,81 autophagy, and endocytosis. Previous research has revealed the inhibition of clathrin-mediated endocytosis, as well as defects in autophagy-related proteins, including Atg5, Atg14, and FIP200, resulting in the aggregation of goblet cell mucin granules.^4,81 Mucin accumulation is not associated with mucin expression, suggesting that this effect might be caused by mucin secretion deficiency.

Recent studies have shown that the secretion of goblet cells relies on autophagy proteins.⁸¹ The MUC2 granule aggregation in the goblet cells is determined via a targeted villin-driven deficiency of the Atg5 autophagy protein in the intestines of mice. This process is mediated by ROS derived from NADPH oxidases.

The NLR protein, NLRP6, is associated with inflammasome signaling and is essential for maintaining intestinal homeostasis.^82,83 In NLRP6 knock out mice, goblet cells were less efficient at secreting mucin and had poorer development of the inner mucus layer.⁸³ No reduction was evident in the specific protein transcription of goblet cells in NLRP6-deficient mice, suggesting that the lack of mucus generation could not be attributed to a decline in transcript production. Conversely, the accumulation of intracellular mucin particles in the distal colon of mice deficient in NLRP6 increased, but these particles failed to merge with the apical surfaces of the goblet cells. NLRP6 deficiency resulted in defective goblet autophagy, reducing mucin secretion into the intestinal lumen.

Recent reports indicated that some goblet cells localized at the colonic crypt entrance underwent nonspecific endocytosis, known as sentinel goblet cells (senGC). Toll-like receptors (TLR)-ligands, LPS, and P3CSK4 were endocytosed by senGC, triggering TLR-MyD88 signaling and inducing downstream ROS synthesis, causing NLRP6 inflammasome-mediated caspase 1 and 11 activation. Furthermore, this led to the Ca2+-dependent exocytosis of MUC2 and intercellular signaling connections, prompting the secretion of MUC2 by the adjacent responsive GCs. The inhibition of endocytosis or NADPH/Dual oxidase ROS synthesis restricted TLR-ligand-induced Muc2 secretion^12,84 (Figure 3).

Figure 3 Regulatory mechanism of mucus secretion in goblet cell and interaction with immune cells. Soluble antigens in the lumen of the intestine such as LPS and P3CSK4 are endocytosed by senGC, triggering TLR-MyD88 signaling, ROS synthesis and NLRP6 inflammasome, causing Ca2+-dependent secretion of MUC2. Goblet cells can also deliver luminal antigens to APCs, initiating adaptive responses.

The Interaction Between Goblet Cells and Immune Cells

Although the main functions of intestinal goblet cells have traditionally been believed to include producing and secreting mucus, recent studies have shown that this is not the case. The intestinal lamina propria (LP) has a large population of dendritic cells, such as CD103- CX3CR1+ antigen-presenting cells (APCs) with macrophage qualities and CD103+ CX3CR1- APCs with dendritic cell characteristics.^85–87 In CD103+ APCs, retinaldehyde dehydrogenase (ALDH1) expression is essential for producing all-trans retinoic acid (ATRA), which plays various roles in the mucosal immune response to lumen antigens, such as promoting IgA responses, imprinting lymphocytes with gut homing, and prompting regulatory T cell formation.^86,88,89 The CD103- CX3CR1+ APCs are crucial for the formation of Th17 T cells, colitis, and the production of TNF-α.⁹⁰ Research has revealed that intestinal epithelial cells can also obtain luminal antigens, presenting them to the dendritic CD103⁺ cells underlying the LP in a way that induced adaptive immune responses, known as goblet-cell-associated antigen passages (GAP cells^86,91). When acetylcholine (ACh) acts on muscarinic acetylcholine receptor 4 (mAChR4) on the goblet cells, GAPs are formed.⁹² The formation of GAPs occurs in a steady state in the small intestine, but is inhibited by Myd88 dependent microbial sensing in the colon. According to Knoop KA, GAP cells could occur in the colon following treatment with antibiotics, that they were repressed by TLR ligands in a goblet cell intrinsic Myd88-dependent manner. The EGFR and MAPK are activated by Myd88, inhibiting the formation of colon GAPs. Therefore, both CD103⁻ and CD103⁺ dendritic cells, as well as subsequent mucosal inflammation, are activated⁹² (Figure 3).

Goblet cell products and luminal antigens are transferred during the interaction with APCs, imprinting them with mucosal properties.⁸⁶ The primary goblet cell product, MUC2, has been shown to imprint anti-inflammatory gene markers required for oral tolerance on APCs.⁹³ Interfering with the APC and epithelial cell interaction reduces the transfer of goblet cell products to APCs, reducing the induction of mucosal reactions.⁹⁴ RELM-β is another product secreted by goblet cells, acting as a chemoattractant recruit CD4 T cells to the colon LP when infected with C. rodentium⁹⁵ CD4+ T cell recruitment to the infected colons of RELM-β knockout mice was restricted, reducing IL-22 production, a pluripotent cytokine directly responsible for enhancing the proliferation of epithelial cells. Goblet cells also regulate the immune response by secreting various cytokines, such as IL25, IL18, IL17, IL15, IL13, IL7, and IL6, and chemokine exotoxin, CCL6, CCL9, and CCL20. The latter attract APCs to the epithelium.^91,94 Therefore, goblet cells establish intimate interactions with immune cells, playing a unique and integral role in maintaining gut immune homeostasis.⁹⁶

Immune Regulation of Goblet Cell Function

Although goblet cells control the LP via a specific mechanism, the immune system is also essential in regulating goblet cell functionality (Figure 4). Type 3 Innate Lymphoid Cells (ILC3s) promotes goblet cell differentiation and the expression of MUC2 through the lymphotoxin (LT)-LTβR pathway during intestinal listeria infection.^10,97 DCs such as macrophages and dendritic cells provide processed, phagocytosed antigens for activating and instructing naïve CD4+ T cells to convert to type 2 helper (Th2) cells.^98,99 These cells are essential for the immune response against extracellular parasites and intracellular pathogens, resulting in the increased secretion of cytokines like IL-13, IL-9, IL-5, and IL-4.^100–102 Of these, IL-4 and IL-13 are considered the major effector cytokines that signal through the IL-4Rα and IL13Rα1 subunits on the intestinal epithelial cells to induce goblet cell hyperplasia via the downstream signal transducer and activator of transcription factor 6 (STAT6) signaling.^103,104 STAT6 is critical for goblet cell hyperplasia development during infection with T. spiralis.^18,105 STAT6 deficient mice infected with T. spiralis failed to generate infection-induced goblet cell hyperplasia. Further studies have shown that IL-13 is crucial in regulating goblet cell hyperplasia in Gymnophalloides seoi infection. The overexpression of IL-13 in mice causes the development of goblet cell hyperplasia in their intestines. Furthermore, the overexpression of exogenous IL-9 and IL-25 promotes goblet cell proliferation and mucin expression via an IL-13-reliant pathway.¹⁰⁶ The administration of IL-4 enhances the thickness and quality of the mucus while decreasing pathogenic contact with the epithelium in C. rodentium and colitis in infected mice.¹⁰⁷ IL-13 and IL-4 upregulate the expression of specific goblet cell products, TFF3 and MUC2, via STAT6 signaling.¹⁰⁸ In addition, IL-13 and IL-4 increase the transcription of MUC2 through the MAPK pathway⁷² (Figure 4).

Figure 4 Immune regulation of goblet cell function and mucin production. (1) IL-33 and IL-25 activate ILC2 and Th2 cells during parasite infections, which release Th2 cytokines such as IL-4, IL-5, IL-9, and IL-13. IL-4 and IL-13 can promote goblet cell proliferation through STAT6 signaling. IL-4 and IL-13 also upregulate the expression of TFF3 and MUC2 via STAT6 or MAPK signaling. IL-25 and IL-9 also promoted goblet cell proliferation and mucin expression through IL-13 dependent pathway. IL-33 induces goblet cell differentiation by stimulating ILCs to produce IL-13. (2) Th1 cytokines such as TNF-α, IL-1β and IFN-γ play complex way in regulating mucin biosynthesis, which not only induce, but also inhibit MUC2 expression in different pathophysiological conditions. (3) IL-22 can regulate goblet cell differentiation and induces mucin expression in STAT3 signaling. IL-10 promotes mucin expression by inhibiting protein misfolding and ER stress in goblet cells.

Like Th2 cytokines, some Th1 cytokines regulate mucin biosynthesis, while TNF-α upregulates MUC2 in human intestinal epithelial cells via the NIK and PI3K/Akt signaling pathways converging at the common NF-κB pathway.⁶⁹ MUC2 was increased in the 3D co-culture model of Caco-2 and HT29-MTX cells when treated with IL-1β, while MUC5AC remained unchanged.¹⁰⁹ By activating PI3K and PKC-MEK/ERK, IL-1β also stimulates the secretion of mucin and the expression of MUC2 genes in the epithelial cells of the human airway.¹¹⁰ In contrast, the Th1 cytokines, TNF-α and IFN-γ, decrease the production of intestinal mucin, as well as the mucin transportation rate from the Golgi to secretory vesicles in the C. rodentium infection mode,¹⁰⁷ implying that the Th1 cytokine impact on goblet cells is not only related to the type of cytokines but also pathological conditions.

Studies have shown that the Th17-associated cytokine, IL-22, is essential in regulating the expression of mucin and the differentiation of GC. IL-22 knockout mice fail to increase the expression of MUC2 and reduced goblet cell hyperplasia in N.brasiliensis and T. muris infection.¹¹¹ This is correlated with the reduced induction of TH2 immunity as IL-4, IL-5, and IL-13 declined.¹¹² In a mouse colitis model, IL-22 was directly responsible for mucin gene expression in the mucosal epithelial cells via goblet cell restitution and STAT3-reliant signaling, alleviating local intestinal inflammation.^113,114

As an anti-inflammatory cytokine, IL-10 inhibits macrophage activation and inflammatory response.^115,116 The expression of IL-10 in normal subjects was higher than in inflammatory bowel disease (IBD) patients.¹¹⁷ IL-10 knockout mice were used for the animal inflammation model.¹¹⁸ The goblet cell count decreased in IL-10 deficient mice compared with wild-type mice.¹¹⁹ Recent studies have also shown that IL-10 has a direct impact on goblet cell mucus production and mucosal characteristics.¹²⁰ Moreover, previous studies suggest that IL-10 restricts ER stress and protein misfolding in goblet cells, enhancing intestinal mucus production.¹²¹

The IL-1 cytokine, IL-33, increases in response to infection and colitis¹²² while prompting the production of the IL-4, IL-5, and IL-13 Th2 cytokines from innate lymphoid cells (ILCs) and T cells.¹²³ IL-33 prevented goblet cell depletion by inhibiting Notch1 signaling in a dextran sulfate sodium (DSS)-induced mouse colitis model.¹²⁴ Research has shown that IL-33 prompts the production of IL-13 by stimulating ILCs, indirectly inducing epithelial goblet cell differentiation.³⁷

Conclusions

Mucin is the primary secretory product of goblet cells and is responsible for generating mucus layers, protecting against pathogen invasion in the intestinal mucosa. These mucus layers are essential in preventing pathogenic microbial invasion and colonization while establishing commensal intestinal microbiota. In recent years, several studies have examined the molecular mechanisms of mucin biology and the regulatory pathways responsible for the secretion and biosynthesis of mucin. This data can help develop new strategies to treat the abnormal mucin expression that is often present in inflammatory and malignant diseases. Furthermore, while the primary function of goblet cells is to maintain the integrity of the intestinal barrier, the complicated contribution of these cells to mucosal immunity far exceeds the mere secretion of mucus. Notably, goblet cells are now considered active participants in defending the host, reacting to their luminal environment in conjunction with the immune response. This review summarizes the crucial nature of the immune system in regulating the biological functions of goblet cells. In conclusion, further research is necessary on how goblet cells control the extracellular environment, interact with the microbiome and its products, and communicate with underlying immunity to clarify the specific mechanisms involved and develop novel therapeutic approaches for intestinal disorders.