Role of Galectin-9 in Atopic Dermatitis — Is It Mediated Through E Selectin? A Clinical and Immunohistochemical Study
Authors Farag AGA, Al-Sharaky DR, Allam SS, Khaled HN
Received 31 August 2019
Accepted for publication 29 November 2019
Published 10 January 2020 Volume 2020:13 Pages 11—19
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Jeffrey Weinberg
Azza Gaber Antar Farag, 1 Dalia Rifaat Al-Sharaky, 2 Sawsan Samy Allam, 3 Hesham Nabil Khaled 1
1Dermatology, Andrology and STDs Department, Faculty of Medicine, Menoufia University, Shebin ElKom, Egypt; 2Pathology Department, Faculty of Medicine, Menoufia University, Shebin ElKom, Egypt; 3Dermatology, Ministry of Health, Damanhour, Behera, Egypt
Correspondence: Azza Gaber Antar Farag
Dermatology, Andrology and STDs, Faculty of Medicine, Menoufia University, Shebin ElKoom, Al Menoufia 32511 Egypt
Fax +20 248 2226454
Background: Atopic dermatitis (AD) is a recognized T helper (Th)2, allergic, skin disease. Galectin-9 (gal-9) is a member of galectin family. It alters T-cell balance resulting in Th2 polarization. These Th2 cells yield various cytokines that may influence E selectin expression. Therefore, we hypothesized that gal-9 may have an active role in AD and this role could be mediated through E selectin.
Objective: To assess this hypothesis, immunohistochemical expression of gal-9 and E selectin was investigated in skin lesions, from atopic dermatitis patients, and compared.
Methods: Twenty-two atopic dermatitis patients and ten controls were included in this case-control study. SCORAD score was used to evaluate atopic dermatitis severity. Biopsies from skin lesions of AD patients and matched sites of controls were taken and stained immunohistochemically by gal-9 and E selectin polyclonal antibodies.
Results: Compared to controls, atopic dermatitis patients exhibited a significant increased gal-9 H score, percent of expression, cellular localization (P˂0.001) and intensity (P=0.04) as well as dermal cellular infiltrate (P˂0.001). Also, there were significant elevations in E selectin H score (P=0.002), percent of expression (P=0.001) and cellular localization (P< 0.001) as well as dermal inflammatory infiltrates in AD cases than controls. In AD, 20 cases showed co expression of both gal-9 and E selectin in the epidermis with insignificant correlation between their H scores.
Study Limitations: This study only included a small number of studied subjects.
Conclusion: Galectin-9 and E selectin participates independently in atopic dermatitis pathogenesis, that may help in development of new therapeutic agents in atopic dermatitis management program.
Keywords: galectins, E- selectin, atopic dermatitis
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