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Role of DiGeorge syndrome critical region gene 9, a long noncoding RNA, in gastric cancer

Authors Ni C, Yang P, Guo J, Ye M

Received 11 January 2018

Accepted for publication 13 March 2018

Published 19 April 2018 Volume 2018:11 Pages 2259—2267

DOI https://doi.org/10.2147/OTT.S162253

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Justinn Cochran

Peer reviewer comments 2

Editor who approved publication: Dr XuYu Yang


Chao Ni,1 Ping Yang,1 Junming Guo,1 Meng Ye2

1Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, People’s Republic of China; 2Department of Medical Oncology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, People’s Republic of China

Introduction: Long non-coding RNAs (lncRNAs) regulate and influence cancer cell development and tumor formation. However, the role for lncRNAs in gastric cancer has not been fully established. In this study, DGCR9, a lncRNA, was significantly upregulated in gastric cancer cell lines.
Methods: The expression levels of DGCR9 in each patient between formalin-fixed, paraffin-embedded (FFPE) gastric cancer tissues and adjacent noncancer tissues (NAT) (n=102) were measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The effect of DGCR9 on cellular proliferation, migration, and glucose uptake was investigated in vitro, respectively.
Results: DGCR9 was shown to have increased expression in gastric cancer tissues and in gastric cancer cell lines. Further, DGCR9 was found to be associated with clinicopathological characteristics of patients with gastric cancer. In particular, DGCR9 was positively associated with lymph node invasion and tumor-node-metastasis (TNM) stage in gastric cancer patients. By in vitro functional analysis, knockdown of DGCR9 in gastric cancer cells suppressed cellular proliferation, migration, and glucose uptake. In contrast, overexpression of DGCR9 increased each of these cancer cell characteristics.
Conclusions: DGCR9 was upregulated in gastric cancer tissues and was shown to accelerate cellular proliferation, migration, and glucose metabolism, all of which would promote the formation and development of gastric cancer.

Keywords: long noncoding RNA, DGCR9, gastric cancer, proliferation, migration, glucose metabolism

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