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Rhesus blood group haplotype frequencies among blood donors in southwestern Uganda

Authors Mbalibulha Y, Muwanguzi E, Mugyenyi G

Received 6 September 2017

Accepted for publication 28 March 2018

Published 19 June 2018 Volume 2018:9 Pages 91—94


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Dr Martin Bluth

Yona Mbalibulha,1 Enoch Muwanguzi,1 Godfrey Mugyenyi2

1Department of Medical Laboratory Sciences, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda; 2Department of Obstetrics and Gynecology, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda

Aim/objective: The study was undertaken to determine the Rhesus blood group system and Rhesus haplotype frequencies among blood donors at Mbarara Regional Blood Bank.
Materials and methods: We included ethylene-diaminetetra-acetic acid-containing plasma samples and serum samples from recruited consented blood donors. The Rh blood group system and the Rh haplotypes was established by the incubation of appropriate antisera (anti-D, anti-E, anti-C, anti-e, and anti-c) and cells at a temperature of 24°C in microplates for 1 hour and the reaction was read by gentle shaking and examining for agglutinations. Donors were asked to fill in questionnaires, after we obtained the informed consent, to assess their demographics.
Results: Among the 386 participants, 233 were males (60%) and 153 (40%) females. The Rh negative blood group percentage was 3.8%, while the Rh haplotype frequencies were as follows: Dce dce 68.1%, dce dce 2.8%, CDe dce 13%, cDE dce 12.4%, DCe DcE 1.6%, DcE DcE 1%, dCe dce 0.8%, and DcE DCe 0.3%.
Conclusion: Given this frequency, a high prevalence of anti-D alloantibody formation among those transfused is possible and could cause diverse effects, especially in the Rh D positive women. We recommend additional research studies on the role of autoimmunity to the transfused on the occurrence of Rh D variants plus their implications on hemolytic disease of the fetus and newborn in Uganda. This study recommends that the blood bank includes Rhesus haplotyping in its protocols and that the finding be disseminated to donors and blood users.

Keywords: Rhesus blood group, Rhesus genotype frequencies, blood donors, Uganda

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