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Retrospective Review of Efficacy and Safety of Anlotinib in Advanced Leiomyosarcoma: A Real-World Study

Authors Zou H, Xia L, Jin G, Wu H, Qian W, Jia D, Xu H, Li T

Received 21 January 2022

Accepted for publication 19 April 2022

Published 11 May 2022 Volume 2022:14 Pages 1703—1711

DOI https://doi.org/10.2147/CMAR.S357334

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Seema Singh



Hanhui Zou, Liming Xia, Gu Jin, Hao Wu, Wenkang Qian, Dongdong Jia, Haichao Xu, Tao Li

Bone and Soft Tissue Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Hangzhou, 310022, People’s Republic of China

Correspondence: Tao Li, Email [email protected]

Objective: Retrospective study on the safety and efficacy of anlotinib in the treatment of advanced leiomyosarcoma in real-world.
Methods: Clinical data were collected from patients suffered from advanced leiomyosarcoma who received anlotinib treatment in Cancer Hospital of the University of Chinese Academy of Sciences from January 2018 to December 2020. Objective response rate (ORR) and disease control rate (DCR) were analyzed according to the RECIST 1.1 criteria. The progression free survival (PFS), overall survival (OS) and adverse reactions were recorded and calculated.
Results: A total of 19 patients (14 female, 5 male) were enrolled, 3 (15.8%) achieved partial response (PR), 11 (57.9%) achieved stable disease (SD), with an ORR of 15.8%, a DCR of 73.7%, a median PFS of 4.1 months (95% CI: 3.0∼ 5.2) and a median OS of 23.5 months (95% CI: 14.2∼ 32.7). The majority of adverse events were grade 1/2, the most common grade 3/4 adverse events were hand-foot syndrome (12.5%), hypertension (5.3%) and oral ulcer (5.3%).
Conclusion: Our results forecast that anlotinib is effective, safe and alternative in treatment of advanced leiomyosarcoma in real-world, combined with immunotherapy may become a potential treatment option. Further, more prospective randomized controlled trials are needed to confirm these findings.

Keywords: leiomyosarcoma, anlotinib, efficacy, adverse events

Introduction

Leiomyosarcoma (LMS) is one of the most common subtypes of malignant mesenchymal neoplasms, accounting for 10% to 20% of all newly diagnosed soft tissue sarcomas (STSs).1 Common locations include the abdomen, larger blood vessels, retroperitoneum and uterus. Surgical resection is the cornerstone treatment for patients with localized LMS, independent of the site of origin. Nevertheless, there is currently no established best first-line treatment for advanced LMS patients.2 In general, LMS show moderate sensitivity to chemotherapy, alternative first-line chemotherapy including doxorubicin-based therapies such as doxorubicin plus dacarbazine and doxorubicin plus ifosfamide,3,4 as well as gemcitabine plus docetaxel;5–7 with an objective response rate (ORR) 18.2%~35.8%, and median progression-free survival (mPFS) 4.4 to 7.4 month. Due to the limited benefit of current treatment, there is an urgent need for clinical treatments to improve survival and safety.

Tumor angiogenesis is a critical process of soft tissue sarcoma tumorigenesis and metastasis.8 Therefore, anti-angiogenic therapy is very important for the treatment of soft tissue sarcoma. Several new drugs for STS have been developed and used in preclinical studies and clinical trials, including regorafenib, pazopanib, anlotinib, etc.9–11 Anlotinib was explored by China as a new small molecule oral multitargeted receptor Tyrosine Kinase Inhibitor (TKI). Anlotinib plays a dual role in anti-tumor angiogenesis and tumor growth through high affinity targeting vascular endothelial growth factor receptor (VEGFR) −1/2/3, platelet-derived growth factor receptor (PGFR) α/β and fibroblast growth factor receptor (FGFR)-1/2/3/4.12,13 Anlotinib has shown encouraging antitumor effects and tolerable toxicity in advanced non-small cell lung cancer (NSCLC), soft tissue sarcoma including leiomyosarcoma, medullary thyroid cancer and metastatic renal clear cell cancer.11,14–16 In a Phase II single-arm multicenter clinical trial,11 a total of 166 relapsed advanced STS patients had a significant survival benefit after treatment with anlotinib, including 26 LMS patients with a 12-week progression free rate of 75% and an objective response rate of 7.7%. The progression free survival and overall survival were 11 and 15 months, respectively. Subsequently, a randomized, double-blind, placebo-controlled phase IIb study (ALTER0203)17 recruited 233 advanced STS patients, showed that anlotinib significantly extended progression-free survival by 4.8 months compared with placebo (6.27 months vs 1.47 months, P< 0.0001). For 41 LMS patients, the median PFS was 5.83 months versus 1.43 months (HR = 0.19, p < 0.0001).

Although the efficacy and safety data obtained with anlotinib in phase II and IIb clinical trials provided important information that led to its approval in advanced soft tissue sarcoma, these data are obtained from patients with rigorous selection criteria. Moreover, there is no relevant research on anlotinib in treatment of patients with advanced leiomyosarcoma. Therefore, we conducted a single-center retrospective study to investigate the efficacy and safety of anlotinib in the treatment of patients with advanced leiomyosarcoma in real world.

Patients and Methods

Patient Characteristics

Clinical date of advanced leiomyosarcoma patients who received anlotinib therapy at Cancer Hospital of the University of Chinese Academy of Sciences were analyzed in this retrospective study. The eligibility criteria were as follows: 1. Histologically confirmed leiomyosarcoma with local advanced stage or distant metastasis; 2. Received at least one cycle of anlotinib treatment; 3. At least one measurable metastatic lesion according to the Response Evaluation Criteria in Solid Tumors (RECIST) version 1.1; 4. Patients who were lost to contact or follow-up were excluded. From January 2018 to December 2020, a total of 19 advanced/metastatic leiomyosarcoma patients were included.

Clinical characteristics including age, sex, ECOG score, primary sites, previous treatment history, duration of initial treatment with anlotinib, combination therapy, time of disease progression, time of drug withdrawal, cause of drug withdrawal, adverse reactions and survival status were recorded. The study was conducted under the supervision of the Ethics Committee of Cancer Hospital of the University of Chinese Academy of Sciences.

Treatment and Evaluation

Advanced LMS patients were treated with anlotinib at an initial oral dose of 12 mg or 10 mg daily for 14 days, and then discontinued for 7 days (3-week cycle), until disease progresses or unacceptable drug toxicity. The anlotinib dose was reduced to 10 mg or 8 mg per day for patients with intolerable or uncontrolled drug-related toxicity. The primary endpoint was progression-free survival (PFS), and the secondary endpoints included overall survival (OS), disease control rate (DCR) and adverse events (AES). PFS was calculated from the date of the first administration of anlotinib until the date of documented disease progression or death from any cause. Tumor responses were assessed according to the RECIST version 1.1 and classified as complete response (CR), partial response (PR), stable disease (SD), or progressive disease (PD). The disease control rate (DCR) was defined as the total percentage of SD, PR and CR. Treatment-related adverse events were assessed according to the Common Terminology Criteria for Adverse Events (CTCAE) version 4.03.

Statistical Analysis

Statistical data were analyzed by SPSS software (version 24.0). Quantitative variables were expressed as medians (range) or numbers (percentage). PFS and OS was draw with Kaplan–Meier method, with 95% confidence interval (CI).

Results

Patient Characteristics

The baseline characteristics of the advanced LMS patients, including age, gender, ECOG performance status, Primary site, surgery history, radiotherapy history, chemotherapy history, initial anlotinib dose, combination therapy, are summarized in Table 1. Five patients were male, and fourteen were female, with a median age of 53 years (range: 30–72 years). A total there were 10 patients with ECOG PS score 0–1 (52.6%), 9 patients with ECOG PS score 2, and no patients with PS score>2. The most common primary site of tumor was retroperitoneal (n=8), followed by extremities (n=5), pelvis (n=4), lung (n=1) and uterus (n=1).

Table 1 Patient Demographics and Clinical Characteristics

The majority of patients were treated with other regiments prior to anlotinib, including 73.7% (14/19) of the patients had received surgery, 31.6% (6/19) of the patients had received radiotherapy, and 73.7% (14/19) of patients had received first-line or above chemotherapy. Five patients received first-line treatment with anlotinib because of poor general condition or refusal to receive chemotherapy. Fifteen patients (78.9%) received 12 mg of anlotinib as the starting dose, and four patients (21.1%) received 10 mg anlotinib as the initial dose. Additionally, seven patients had received other nonsurgical treatments, including chemotherapy, radiotherapy and immunotherapy.

Efficacy

All patients were followed up to March 2021, with a median follow-up time of 17.8 months (range 4.3–23.5). In all 19 patients, none achieved CR, 3 patients achieved PR and 11 patients experienced SD, yielding an overall ORR of 15.8% (3/19) and DCR of 73.7% (14/21). By the end of the follow-up, all patients showed disease progression and 12 patients died, with a median PFS of 4.1 months (Figure 1A; 95% CI: 2.96–5.24), and the median OS of 23.5 months (Figure 1B; 95% CI: 12.3–34.7) (Table 2).

Figure 1 Progression-free survival (A) and Overall survival (B) of all patients.

Table 2 Overall Response to Treatment

The clinical characteristics of anlotinib therapy in this study are detailed in Table 3. The average change in target lesion size compared with baseline is shown in Figure 2. Tumor retraction was observed in 8 patients (42.1%), with an average tumor reduction rate of 31.7%. Tumor retraction rate was more than 70% in 2 patients treated with anlotinib combined with immunotherapy.

Table 3 Clinical Characteristics of Patient with Leiomyosarcoma Treated with Anlotinib

Figure 2 Tumor changes from baseline in patients with leiomyosarcoma treated with anlotinib (RECIST 1.1 criteria).

Safety and Toxicity

Treatment-related adverse events are summarized in Table 4. The most common 1–2 grade adverse reactions included fatigue (6, 31.6%), hand and foot syndrome (5, 26.3%), and hypertension (5, 26.3%). Grade 3/4 adverse events occurred in 4 patients, including Hand foot skin syndrome (2, 10.5%), hypertension (1, 5.3%), and Oral ulcers (1, 5.3%). All adverse events can be relieved by symptomatic treatment, reduction of anlotinib dose, or discontinuation of treatment. Five patients (26.3%) reduced the dosage of anlotinib, and two patients (10.5%) discontinued medication due to severe adverse reactions. No treatment-related deaths have occurred.

Table 4 Adverse Events

Discussion

Treatment of leiomyosarcoma, especially advanced leiomyosarcoma, has always been a challenge in clinical work. Over the past few decades, doxorubin-based regimens and gemcitabine-based regimens are still the backbone of early-line therapy for metastatic disease.18 When patients fail in first-line chemotherapy, there is no standard second-line treatment option in China, and few therapeutic drugs are available for treatment. As a newly novel tyrosine kinase inhibitor independently developed in China, anlotinib has shown promising antitumor activity in many advanced STS. Based on the results of phase II study11 and phase IIb study (ALTER0203),17 anlotinib has been approved by China Food and Drug Administration (CFDA) for the treatment of advanced alveolar soft-part sarcoma and clear cell sarcoma as first-line systemic therapy, and other advanced STS after the failure of anthracycline-based chemotherapy.

As far as we know, our study is the first real-world study to evaluate the efficacy and safety of anlotinib in advanced leiomyosarcoma. In this study, the ORR and DCR of all patients were 15.8% and 73.7%, similar with the phase II study11 (ORR: 13%, DCR: 74%), a slightly higher than phase IIb (ALTER0203) clinical study17 (ORR: 10.1%, DCR:55.7%), and also the ORR (13.79%) and DCR (55.17%) in a retrospective study reported by Tian et al.19 Admittedly, the results of these clinical studies included a variety of STSs, and secondly, our study included 5 patients (26.3%) who had not received first-line chemotherapy, and 7 patients (36.8%) received combination therapy during the treatment of anlotinib. Although in the subgroup analysis, there was no significance benefit in ORR or DCR in the combination group compared with the anlotinib monotherapy group, a higher rate of PR was achieved in the combination group (2/7, 28.6%) than in the anlotinib monotherapy group (1/12, 8.3%). The median PFS of this study from the beginning of anlotinib treatment was 4.1 months, which were numerically lower than the median PFS of LMS group in phase II11 (11 months) and phase IIb17 (5.83 months) clinical studies. As a real-world study, the general condition of the patients with leiomyosarcoma we included was relatively poor, with 47.4% (9/19) of patients with PS score ≥ 2 and 87.5% (16/19) of patients with distant metastasis of other organs. Of note, although the data of this study showed that the median OS of LMS patients was 23.5 months, significantly longer than the results of most clinical studies, we believed that this was mainly due to insufficient sample size and follow-up time, and the median OS data tended to decline with the extension of follow-up time.

In the analysis of gender, PS score, treatment history, primary site, combination therapy and other subgroups, we did not find significant differences in PFS and OS, which was mainly restricted by the small number of samples. Of our concern, we found that of the 3 patients treated with anlotinib combined with immunotherapy (Pembrolizumab 100mg every 3 weeks), two patients reached PR, another was SD, and two patients with PR achieved a tumor retraction rate of more than 70% and were still alive by the end of follow-up. The efficacy of immunotherapy in leiomyosarcoma has been controversial, a one-arm study of nivolumab in patients with advanced uterus LMS, which closed after the first stage of accrual (12 patients) for lack of efficacy, showing no objective responses and a median PFS of 1.8 months.20 Another pooled analysis of clinical trials investigating a PD1 or PD-L1 antagonist in patients with advanced STS also come to conclusion of leiomyosarcoma exhibited the lowest response rates to immunotherapy.21 Interestingly, in a retrospective analysis of the efficacy of Immunotherapy in metastatic soft-tissue sarcomas of Monga et al forty-five percent (9 of 20) of LMS patients achieved a PR during anti-PD-1 therapy, observed a notable response rate in LMS subtypes.22 Meanwhile, a few case reports response to checkpoint inhibition in LMS were published.23,24 STS exhibit low expression of factors related to immune response, which could explain the modest activity of PD-1 inhibitors. Vascular endothelial growth factor promotes the immunosuppressive tumor microenvironment and contributes to Immune checkpoint inhibitors resistant therapy.25 Phase I/IIb clinical trial evaluating the combination of sunitinib and nivolumab in advanced sarcomas, although LMS subtype was not included, the 6-month progression-free survival rate was 48%, overall response rate of 21% (12 out of 58).26 Anlotinib combined with pd-1 antibody also reported in STSs recently. In a single-center retrospective analysis,27 61 advanced STS patients were treated with ICIs alone or combined with TKIs, the median PFS was significantly prolonged after ICI treatment in combination with TKIs (11.74 months) compared to ICI treatment alone (6.81 months), with a 12-month PFS rate of 42.9% and overall response rate of 30% in ICI-TKI combination group. We speculate that anlotinib combined with pd-1 inhibitors can play a synergistic role in the treatment of LMS, which still needs to be confirmed by relevant clinical and basic studies.

The safety and tolerability during the treatment of anlotinib were consistent with extensive prior experience and reports.11,17,19,28,29 The most frequent adverse events were hand-foot syndrome, fatigue and hypertension, most of which were mild and reversible. The incidence of grade 3/4 adverse reactions was 21.1%, oncologists should be vigilant about the occurrence of dose reduction or drug withdrawal when combined with other antitumor therapies.

Additionally, we acknowledge several limitations of this single-institution retrospective study. First of all, this was a small-sample study. Second, as a retrospective study, not all possible clinical data can be reliably retrieved from records, which may lead to recall bias. Finally, the follow-up period was not long enough to determine the overall survival of all patients.

Conclusions

In summary, our results indicate that anlotinib is reliable, safe and feasible for advanced leiomyosarcoma. Anlotinib combined with immunotherapy may become a potential treatment option. Ultimately, further long-term randomized controlled trials with larger sample sizes are needed to fully characterized the clinical application of anlotinib in LMS.

Ethics Statement

The studies involving human participants were reviewed and approved by Ethics Committee of Cancer Hospital of the University of Chinese Academy of Sciences. As a non-intervention retrospective study, it has been approved to waive informed consent. Privacy and identity information of all patients were confidentiality and compliance with the Declaration of Helsinki.

Disclosure

The authors report no conflicts of interest in this work.

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