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Renal Transcriptomics Reveals the Carcinogenic Mechanism of Ethyl Carbamate in Musalais

Authors Wang W, Han ZJ, Guo D, Xiang Y

Received 12 October 2020

Accepted for publication 18 January 2021

Published 25 February 2021 Volume 2021:14 Pages 1401—1416

DOI https://doi.org/10.2147/OTT.S282125

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Sanjay Singh


Weihua Wang, ZhanJiang Han, Dongqi Guo, Yanju Xiang

College of Life Science, Tarim University, Xinjiang Uygur Autonomous Region, Alaer City, 843300, People’s Republic of China

Correspondence: ZhanJiang Han
College of Life Science, Tarim University, No. 1188, Military Reclamation Avenue, Xinjiang Uygur Autonomous Region, Alaer City, 843300, People’s Republic of China
Tel +86 18742641847
Email [email protected]

Introduction: Musalais is a traditional fermented wine produced in southern Xinjiang (a province of China) and is protected as a form of national intangible cultural heritage. However, ethyl carbamate (EC), which is naturally produced during the fermentation process, has been shown to induce carcinogenesis and was classified as a group 2A carcinogen by The World Health Organization’s International Agency for Research on Cancer.
Methods: In this work, rats were treated with musalais containing EC at varying contents (0.1, 1, or 10 mg/kg). To evaluate the toxicity of EC in musalais, the liver and kidney of the rats were subjected to transcriptomics sequencing. Differentially expressed genes (DEGs) between treated and untreated rats were identified, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed on these genes to investigate the biological functions affected by EC in musalais.
Results: The results demonstrated that high EC content in musalais is possibly involved in the regulation of cytochrome P450 metabolism, chemical carcinogenesis, metabolism of xenobiotics by cytochrome P450, Wnt signaling, and p53 signaling by targeting Mgst1, Gstp1, Gsta5, Gsta1, Adh1, Gsta2, and Ccnd1, thereby inducing cancer.
Conclusion: The present work predicted the potential carcinogenic mechanism of high EC content in musalais, providing a reference for its safety evaluation.

Keywords: musalais, ethyl carbamate, transcriptomics sequencing, toxicity prediction, mechanism research

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