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Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells

Authors Mouratidis P, George AJT

Received 4 February 2015

Accepted for publication 13 March 2015

Published 21 May 2015 Volume 2015:8 Pages 97—106

DOI https://doi.org/10.2147/JIR.S82202

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 5

Editor who approved publication: Dr Ning Quan


Petros XE Mouratidis,* Andrew JT George*
 
Department of Immunology, Imperial College London, London, UK

*These authors contributed equally to this work


Background: Indoleamine 2,3-dioxygenase (IDO) is an enzyme associated with the regulation of immune responses. Cytokines such as IFNγ induce its expression in endothelial cells originating from immune-privileged sites. In this study, we investigate regulators of IDO in primary endothelial cells from a non-immune-privileged site and determine whether IDO expression affects immune cell behavior.
Methods: IDO expression was determined using real-time quantitative polymerase chain reaction and immunoblotting. IDO activity was estimated using an IDO enzyme assay. Primary cells were transfected using microporation, and T-cell migration was determined using a cell transmigration assay.
Results: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ but not after treatment with TNFα, IL-1β, IL-2, IL-4, IL-6, or IL-10. VEGFβ and heparin negatively regulate IFNγ-driven increases in IDO. Overexpression of IDO in endothelial cells does not affect transmigration of T-cells.
Conclusion: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ. Heparin and angiogenesis stimulators such as VEGFβ negatively regulate its expression.

Keywords: IFNγ, VEGFβ, endothelium, inflammation, HSVEC

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