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PWRN1 Suppressed Cancer Cell Proliferation and Migration in Glioblastoma by Inversely Regulating hsa-miR-21-5p

Authors Jiang J, Wang X, Lu J

Received 19 February 2020

Accepted for publication 26 May 2020

Published 2 July 2020 Volume 2020:12 Pages 5313—5322

DOI https://doi.org/10.2147/CMAR.S250166

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly


Jianxin Jiang,* Xiaolin Wang,* Jun Lu

Department of Neurosurgery, Taizhou People’s Hospital, Taizhou, Jiangsu Province 225300, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jun Lu
Department of Neurosurgery, Taizhou People’s Hospital, M.D 366 Taihu Road, Taizhou, Jiangsu Province 225300, People’s Republic of China
Tel +86-523-89890321
Email lujun83@aol.com

Objective: To evaluate the expression and function of long noncoding RNA (lncRNA) Prader-Willi region non-protein coding RNA 1 (PWRN1) in human glioblastoma (GBM).
Materials and Methods: QRT-PCR was applied to assess PWRN1 expression in human GBM tumors and GBM cell lines. PWRN1 was overexpressed by lentiviral infection in LN-229 and U-251 cells to evaluate its effect on GBM cell proliferation and migration in vitro, and xenograft in vivo. The endogenously competing target of PWRN1, human microRNA-21-5p (hsa-miR-21-5p) was evaluated by dual-luciferase activity assay and qRT-PCR. Also, hsa-miR-21-5p was upregulated in PWRN1-overexpressed GBM cells to evaluate the functional involvement of hsa-miR-21-5p in PWRN1-mediated GBM cell proliferation and migration.
Results: PWRN1 was downregulated in both human GBM tumors and GBM cell lines. In LN-229 and U-251, PWRN1 overexpression suppressed cancer cell proliferation and migration in vitro, and xenograft growth in vivo. Hsa-miR-21-5p was demonstrated to be the downstream competing target of PWRN1 in GBM. Conversely, upregulating hsa-miR-21-5p in LN-229 and U-251 cells reversed the tumor-suppressing effects of PWRN1 overexpression.
Conclusion: PWRN1 is markedly downregulated in GBM. Overexpressing PWRN1 has tumor inhibitory effect on GBM cells, likely via reversely suppressing the expression of tumor oncogenic factor of hsa-miR-21-5p.

Keywords: GBM, lncRNAs, PWRN1, microRNA, hsa-miR-21-5p, migration

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