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Protein immobilization onto electrochemically synthesized CoFe nanowires
Authors Torati SR, Reddy V, Yoon SS, Kim C
Received 1 November 2014
Accepted for publication 26 November 2014
Published 14 January 2015 Volume 2015:10(1) Pages 645—651
DOI https://doi.org/10.2147/IJN.S76850
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Prof. Dr. Thomas J. Webster
Sri Ramulu Torati,1 Venu Reddy,1 Seok Soo Yoon,2 CheolGi Kim1
1Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea; 2Department of Physics, Andong National University, Andong, South Korea
Abstract: CoFe nanowires have been synthesized by the electrodeposition technique into the pores of a polycarbonate membrane with a nominal pore diameter of 50 nm, and the composition of CoFe nanowires varying by changing the source concentration of iron. The synthesized nanowire surfaces were functionalized with amine groups by treatment with aminopropyltriethoxysilane (APTES) linker, and then conjugated with streptavidin-Cy3 protein via ethyl (dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide coupling chemistry. The oxide surface of CoFe nanowire is easily modified with aminopropyltriethoxysilane to form an amine terminating group, which is covalently bonded to streptavidin-Cy3 protein. The physicochemical properties of the nanowires were analyzed through different characterization techniques such as scanning electron microscope, energy dispersive spectroscopy, and vibrating sample magnetometer. Fluorescence microscopic studies and Fourier transform infrared studies confirmed the immobilization of protein on the nanowire surface. In addition, the transmission electron microscope analysis reveals the thin protein layer which is around 12–15 nm on the nanowire surfaces.
Keywords: electrodeposition, biofunctionalization, streptavidin
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