Back to Journals » Infection and Drug Resistance » Volume 5

Prospective evaluation of simply modified MODS assay: an effective tool for TB diagnosis and detection of MDR-TB

Authors Chaiyasirinroje B, Aung MN, Moolphate, Kasetjaroen Y, Rienthong S, Dhanida, Nampaisan O, Nedsuwan S, Wiravoot, Suriyon, Mitarai S, Yamada N

Received 16 July 2011

Accepted for publication 12 December 2011

Published 7 May 2012 Volume 2012:5 Pages 79—86


Review by Single-blind

Peer reviewer comments 2

Boonchai Chaiyasirinroje1,*, Myo Nyein Aung2,3,*, Saiyud Moolphate1,4, Yuthichai Kasetjaroen5, Somsak Rienthong5, Dhanida Rienthong5, Oranuch Nampaisan1, Supalert Nedsuwan6, Wiravoot Sangchun6, Narin Suriyon7, Satoshi Mitarai4, Norio Yamada4

1TB/HIV Research Project, RIT, Chiang Rai, Thailand; 2Department of Public Health, Graduate School of Medicine, Juntendo University, Tokyo, Japan; 3Boromrajonani College of Nursing Nakhon Lampang (BCNLP), Lampang, Thailand; 4Research Institute of Tuberculosis (RIT), Tokyo, Japan; 5Bureau of Tuberculosis, Bangkok, Thailand; 6Chiang Rai Regional Hospital, Chiang Rai, Thailand; 7Chiang Rai Provincial Health Office, Chiang Rai, Thailand

*These authors contributed equally to this work

Background and setting: Thailand is one of the highest tuberculosis (TB)-burdened countries. Chiang Rai, the northernmost province of Thailand has high tuberculosis and human immunodeficiency virus (HIV) prevalence and the laboratory workload for TB culture and drug susceptibility testing is increasing.
Objectives: To evaluate the simply modified microscopic-observation drug-susceptibility assay (MODS) in the setting of a developing country.
Methods: In this cross-sectional diagnostic study, a total of 202 sputum samples of clinically diagnosed TB patients were used to test the performance of MODS assay in reference to gold standard BACTEC™ MGIT™ 960 liquid culture system and Ogawa solid culture. Sputum samples were collected from clinically diagnosed TB patients. Culture growth rate and time to culture positivity were compared among three methods. Performance of modified MODS assay was evaluated for detection of mycobacterium drug resistance in reference to MGIT antimicrobial susceptibility test (AST).
Result: Median time to culture positivity by MODS, solid, and liquid culture were 12, 30, and 6 days respectively. Compared to the drug susceptibility test (DST) result of reference liquid culture, the sensitivity and specificity of MODS for detection of multidrug-resistant tuberculosis (MDR-TB) was 85.7% and 97.5% respectively. MODS assay has a positive predicative value of 80% and negative predictive value of 96.5% for isoniazid resistance, 70% and 100% for rifampicin resistance, and 66.7% and 99.1% for MDR-TB.
Conclusion: MODS is a highly effective screening test for detection of MDR-TB.

Keywords: tuberculosis, drug resistance, MODS assay, Chiang Rai, Thailand

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other articles by this author:

The social network index and its relation to later-life depression among the elderly aged ≥80 years in Northern Thailand

Aung MN, Moolphate S, Aung TNN, Katonyoo C, Khamchai S, Wannakrairot P

Clinical Interventions in Aging 2016, 11:1067-1074

Published Date: 8 August 2016

Assessment of preclinical students’ academic motivation before and after a three-day academic affair program

Aung MN, Somboonwong J, Jaroonvanichkul V, Wannakrairot P

International Journal of General Medicine 2015, 8:373-380

Published Date: 15 December 2015

Causes of mortality among tuberculosis and HIV co-infected patients in Chiang Rai, Northern Thailand

Kantipong P, Murakami K, Moolphate S, Aung MN, Yamada N

HIV/AIDS - Research and Palliative Care 2012, 4:159-168

Published Date: 4 October 2012

Public knowledge of diabetes in Karen Ethnic rural residents: a community-based questionnaires study in the far north-west of Thailand

Lorga T, Srithong K, Manokulanan P, Aung TNN, Aung MN

International Journal of General Medicine 2012, 5:799-804

Published Date: 27 September 2012

Assessing awareness and knowledge of hypertension in an at-risk population in the Karen ethnic rural community, Thasongyang, Thailand

Aung MN, Lorga T, Srikrajang J, Promtingkran N, Kreuangchai S, Tonpanya W, Vivarakanon P, Jaiin P, Praipaksin N, Payaprom A

International Journal of General Medicine 2012, 5:553-561

Published Date: 27 June 2012

Predicting prediabetes in a rural community: a survey among the Karen ethnic community, Thasongyang, Thailand

Lorga T, Aung MN, Naunboonruang P, Thinuan P, Praipaksin N, Deesakul T, Inwan U, Yingtaweesak T, Manokulanan P, Suangkaew S, Payaprom A

International Journal of General Medicine 2012, 5:219-225

Published Date: 1 March 2012

Time of highest tuberculosis death risk and associated factors: an observation of 12 years in Northern Thailand

Saiyud Moolphate, Myo Nyein Aung, Oranuch Nampaisan, et al

International Journal of General Medicine 2011, 4:181-190

Published Date: 28 February 2011

Readers of this article also read:

Emerging and future therapies for hemophilia

Carr ME, Tortella BJ

Journal of Blood Medicine 2015, 6:245-255

Published Date: 3 September 2015

Is increasing the dose of Entecavir effective in partial virological responders?

Erturk A, Adnan Akdogan R, Parlak E, Cure E, Cumhur Cure M, Ozturk C

Drug Design, Development and Therapy 2014, 8:621-625

Published Date: 29 May 2014

A novel preparation method for silicone oil nanoemulsions and its application for coating hair with silicone

Hu Z, Liao M, Chen Y, Cai Y, Meng L, Liu Y, Lv N, Liu Z, Yuan W

International Journal of Nanomedicine 2012, 7:5719-5724

Published Date: 12 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012