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Proliferation and differentiation of human fetal brain neural stem cells in vitro

Authors McGrath EL, Gao J, Wu P

Received 10 August 2017

Accepted for publication 16 November 2017

Published 10 January 2018 Volume 2018:6 Pages 19—27

DOI https://doi.org/10.2147/JN.S148794

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Hongyun Huang


Erica L McGrath,1 Junling Gao,1 Ping Wu1,2

1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, USA; 2Beijing Institute for Brain Disorders, Capital Medical University, Beijing, People’s Republic of China

Abstract: Primary human fetal brain neural stem cells (hNSCs) are a unique non-genetically modified model system to study molecular mechanisms underlying human neural development, to model human diseases, and to screen drugs or validate new treatments. They may also be used for cell transplantation to treat various neurological diseases. This protocol details our methods that can be used to expand hNSCs in culture as well as how to differentiate them into various neuronal lineages and astrocytes.

Keywords: neural stem cell, proliferation, differentiation, neuron, astrocyte

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