Prognostic value of microRNA-126 and CRK expression in gastric cancer
Authors Yue S, Shi H, Han J, Zhang T, Zhu W, Zhang D
Received 1 May 2015
Accepted for publication 13 July 2015
Published 11 October 2016 Volume 2016:9 Pages 6127—6135
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Manfred Beleut
Peer reviewer comments 4
Editor who approved publication: Professor Daniele Santini
Shun Yue,1,* Huichang Shi,2,* Jun Han,3 Tiecheng Zhang,1 Weiguo Zhu,1 Dahong Zhang1
1Department of Medical Oncology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an City, 2Department of Medical Oncology, The Second People’s Hospital of Huai’an, Huai’an City, 3Department of Medical Oncology, Qinghai Province People’s Hospital, Xining City, People’s Republic of China
*These authors contributed equally to this work
Background: MicroRNA (miR)-126, acting as a tumor suppressor, has been reported to inhibit the invasion of gastric cancer cells in part by targeting v-crk sarcoma virus CT10 oncogene homologue (CRK). The aim of this study was to investigate the clinical significance of miR-126/CRK axis in gastric cancer.
Methods: miR-126 and CRK mRNA expression levels were detected by real-time quantitative reverse transcription polymerase chain reaction in 220 self-pairs of gastric cancer and adjacent noncancerous tissues.
Results: Expression levels of miR-126 and CRK mRNA in gastric cancer tissues were, respectively, lower and higher than those in adjacent noncancerous tissues (both P<0.001). Low miR-126 expression and high CRK expression, alone or in combination, were all significantly associated with positive lymph node and distant metastases and advanced TNM stage of human gastric cancer (all P<0.05). We also found that the overall survival rates of the patients with low miR-126 expression and high CRK expression were, respectively, shorter than those with high miR-126 expression and low CRK expression. Interestingly, miR-126-low/CRK-high expression was associated with a significantly worse overall survival of all miR-126/CRK groups (P<0.001). Moreover, multivariate analysis identified miR-126 and/or CRK expression as independent prognostic factors for patients with gastric cancer. Notably, the prognostic relevance of miR-126 and/or CRK expression was more obvious in the subgroup of patients with TNM stage IV.
Conclusion: Dysregulation of miR-126/CRK axis may promote the malignant progression of human gastric cancer. miR-126 and CRK combined expression may serve as an independent predictor of overall survival in patients with advanced gastric cancer.
Keywords: miR-126, v-crk sarcoma virus CT10 oncogene homologue, prognosis, real-time quantitative polymerase chain reaction
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