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Prognostic and Clinicopathological Significance of EphB3 and Dysadherin Expression in Extrahepatic Cholangiocarcinoma

Authors Wu Z, Liu R, Xiong L, Miao X, Li D, Zou Q, Yuan Y, Yang Z

Received 24 September 2019

Accepted for publication 30 December 2019

Published 10 January 2020 Volume 2020:12 Pages 221—232

DOI https://doi.org/10.2147/CMAR.S232278

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Eileen O'Reilly


Zhengchun Wu, 1 Rushi Liu, 2 Li Xiong, 1 Xiongying Miao, 1 Daiqiang Li, 3 Qiong Zou, 4 Yuan Yuan, 4 Zhulin Yang 1

1Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People’s Republic of China; 2Laboratory of Medical Molecular and Immunological Diagnostics, School of Medicine, Hunan Normal University, Changsha, Hunan 410013, People’s Republic of China; 3Department of Pathology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People’s Republic of China; 4Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, People’s Republic of China

Correspondence: Zhulin Yang
Department of General Surgery, Second Xiangya Hospital, Central South University, 139 Renmin Road, Changsha, Hunan 410011, People’s Republic of China
Email yangzhulin8@csu.edu.cn

Aim: EphB3 and dysadherin are involved in tumorigenesis and progression of many neoplasms. However, the roles of EphB3 and dysadherin in extrahepatic cholangiocarcinoma (ECC) remain to be revealed. In this study, we aimed to evaluate the expression of EphB3 and dysadherin, and investigate their clinicopathological significance in ECC.
Methods: We examined EphB3 and dysadherin expression in 100 ECC, 30 peritumoral tissues, 10 adenoma and 15 normal biliary tract tissues using EnVision immunohistochemistry. The relationship between EphB3 or dysadherin expression and clinicopathological features was evaluated using the χ 2 test or Fisher’s exact test. The overall survival of ECC patients was analyzed using Kaplan-Meier univariate survival analysis and Log rank tests.
Results: We found that EphB3 expression was significantly down-regulated and dysadherin expression was significantly up-regulated in ECC tissues compared with normal tissues (P < 0.01). EphB3 expression was negatively correlated with dysadherin expression in ECC (P < 0.01). The positive rate of EphB3 expression and negative rate of dysadherin expression was significantly higher in patients with well-differentiated type, no lymph node metastasis, no surrounding tissues and organs invasion, early TNM stages (I + II) and radical resection (P < 0.01). The survival of ECC patients with positive EphB3 or negative dysadherin expression was significantly longer than patients with negative EphB3 or positive dysadherin expression (P < 0.01). Cox multivariate analysis demonstrated that negative EphB3 or positive dysadherin expression were independent poor prognostic factors in ECC patients. The ROC curves suggested that EphB3 and dysadherin combined diagnostic efficacy (AUC=0.688, 95%CI: 0.603-0.772) was significantly higher EphB3 diagnostic efficacy (AUC=0.654, 95%CI: 0.564-0.743) or dysadherin diagnostic efficacy (AUC=0.648, 95%CI: 0.558-0.737) alone.
Conclusion: EphB3 and dysadherin are involved in the carcinogenesis and progression of ECC, and ECC patients with negative EphB3 or positive dysadherin expression have a poor prognosis.

Keywords: extrahepatic cholangiocarcinoma, EphB3, dysadherin, prognosis, clinicopathological significance


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