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Prevalence and Risk Factors of Mastitis and Isolation, Identification and Antibiogram of Staphylococcus Species from Mastitis Positive Zebu Cows in Toke Kutaye, Cheliya, and Dendi Districts, West Shewa Zone, Oromia, Ethiopia

Authors Dabele DT, Borena BM Snr, Admasu P, Gebremedhin EZ, Marami LM

Received 2 December 2020

Accepted for publication 3 March 2021

Published 12 March 2021 Volume 2021:14 Pages 987—998

DOI https://doi.org/10.2147/IDR.S295257

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Professor Suresh Antony


Dimshasha Tolera Dabele,1 Bizunesh Mideksa Borena Snr,2 Petros Admasu,2 Endrias Zewdu Gebremedhin,2 Lencho Megersa Marami3

1Asella Regional Veterinary Laboratory, Asella, Oromia Regional State, Ethiopia; 2Department of Veterinary Science, Ambo University, Ambo, Oromia Regional State, Ethiopia; 3Department of Veterinary Laboratory Technology, Ambo University, Ambo, Oromia Regional State, Ethiopia

Correspondence: Bizunesh Mideksa Borena
Ambo University, P.O. Box 19, Ambo, Ethiopia
Tel +251- 911832048
Fax +251- 112609503
Email [email protected]

Purpose: Mastitis is one of the major global problems severely affecting the dairy sector. Staphylococcus species are the primary bacteria consistently identified from mastitic milk. This study was aimed to estimate the prevalence of mastitis, isolate Staphylococcus species, determine risk factors, and the antimicrobial susceptibility profile of Staphylococcus species from mastitic Zebu cows in West Shewa Zone, Ethiopia.
Materials and Methods: A total of 404 lactating Zebu cows were tested for mastitis. Isolation and identification of Staphylococcus from mastitis positive samples were done by bacteriological culture and biochemical tests. Further identification of coagulase-negative Staphylococcus (CNS) species and antimicrobial susceptibility test of the Staphylococcus aureus and the CNS was done by the Phoenix machine. Descriptive statistics was used to summarize the prevalence of mastitis while the Chi-square test and logistic regression were used to determine the association between the prevalence of mastitis and the risk factors and the magnitude of association, respectively.
Results: The present study showed an overall cow and quarter level mastitis prevalence of 30.5% (95% confidence interval [CI]:26.0– 35.2) and 8.3% (95% CI 7.0– 9.8), respectively. The quarter level isolation rate of Staphylococcus species was 38.6% (95% CI: 30.1– 47.6). Five Staphylococcus species namely S. intermedius, S. hyicus, S. aureus, S. lentus, and S. sciuri were identified. The latter two are CNS and were identified for the first time in Ethiopia. Antimicrobial susceptibility testing showed none of the isolates of S. aureus, 100% of S. sciuri, and 87.5% of S. lentus species were multidrug-resistant. The independent predictors of mastitis (p< 0.05) were the age of the cows, stage of lactation, type of housing, the interval of bedding cleaning, and previous history of mastitis.
Conclusion: The study showed a high prevalence of mastitis, Staphylococcus species, and multidrug resistant S. lentus, and S. sciuri in Zebu cows.

Keywords: antimicrobial resistance, mastitis, prevalence, risk factor, Staphylococcus, Zebu cows

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