Back to Journals » International Journal of Nanomedicine » Volume 7

Preparation and in vitro studies of MRI-specific superparamagnetic iron oxide antiGPC3 probe for hepatocellular carcinoma

Authors Li Y, Chen Z, Li F, Wang J, Zhang Z

Received 24 March 2012

Accepted for publication 31 May 2012

Published 22 August 2012 Volume 2012:7 Pages 4593—4611

DOI https://doi.org/10.2147/IJN.S32196

Review by Single-blind

Peer reviewer comments 2

Youwei Li,1,2 Zhengguang Chen,3 Fei Li,1 Jichen Wang,4 Zongming Zhang1,5

1
School of Medicine, Tsinghua University, 2Department of Radiology, Beijing Chuiyangliu Hospital, 3Department of Radiology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing; 4Department of Radiology, Nanjing BenQ Hospital, Nanjing, 5Department of Hepatobiliary Surgery, Futian Hospital, Guangdong Medical College, Shenzhen, People's Republic of China

Background: The aim of this study was to develop an antiGPC3-ultrasuperparamagnetic iron oxide (USPIO) probe for early detection of hepatocellular carcinoma.
Methods: GPC3 and AFP receptors were selected as biomarkers and conjugated with USPIO nanoparticles coated by dextran with carboxylate groups to synthesize antiGPC3-USPIO and antiAFP-USPIO probes. HepG2 cells (a human hepatocellular carcinoma cell model with high expression of GPC3) were used along with SMMC-7721 cells (a hepatocellular carcinoma cell model with no expression of GPC3), HeLa cells (a cervical cancer model), and HL-7702 (normal hepatocytes) which were used as controls. After incubation with the probes, the iron content in the cells was calculated, USPIO nanoparticles in cells were observed using transmission electron microscopy, and T1 and T2 relaxation times were measured with a 1.5 T magnetic resonance scanner.
Results: AntiGPC3-USPIO probes with a mean hydrodynamic diameter of 47 nm showed good biological compatibility. Transmission electron microscopic images indicated that the amount of USPIO nanoparticles taken up was significantly higher in HepG2 cells incubated with antiGPC3-USPIO than that in HepG2 cells incubated with antiAFP-USPIO or USPIO nanoparticles and that in the SMMC-7721 or HeLa cells incubated with antiGPC3-USPIO probes, antiAFP-USPIO probes, or USPIO nanoparticles. The higher the concentration and the longer the incubation time, the greater the number of USPIO nanoparticles found in the cells. No USPIO nanoparticles were found in the HL-7702 cells. All of the HepG2, SMMC-7721, and HeLa cells incubated with antiGPC3-USPIO, antiAFP-USPIO, or USPIO nanoparticles were able to shorten the T1 and T2 values in agar solution, especially the T2 images of HepG2 cells incubated with antiGPC3-USPIO probes.
Conclusion: AntiGPC3-USPIO probes can be utilized as a specific magnetic resonance targeting contrast agent for early detection of hepatocellular carcinoma. Using a 1.5 T magnetic resonance scanner, the optimal time for imaging HepG2 cells was around 2–4 hours after incubation with antiGPC3-USPIO probes.

Keywords: magnetic resonance imaging, hepatocellular carcinoma, HepG2 cells, superparamagnetic iron oxide antiGPC3-USPIO probe

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Readers of this article also read:

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Karagkiozaki V, Karagiannidis PG, Kalfagiannis N, Kavatzikidou P, Patsalas P, Georgiou D, Logothetidis S

International Journal of Nanomedicine 2012, 7:6063-6076

Published Date: 17 December 2012

Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study

Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A

International Journal of Nanomedicine 2012, 7:6003-6009

Published Date: 5 December 2012

Encapsulation of cisplatin in long-circulating and pH-sensitive liposomes improves its antitumor effect and reduces acute toxicity

Leite EA, Souza CM, Carvalho-Júnior AD, Coelho LG, Lana AM, Cassali GD, Oliveira MC

International Journal of Nanomedicine 2012, 7:5259-5269

Published Date: 9 October 2012

Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells

Wang K, Zhang T, Liu L, Wang XL, Wu P, Chen ZG, Ni C, Zhang JS, Hu FQ, Huang J

International Journal of Nanomedicine 2012, 7:4487-4497

Published Date: 13 August 2012

The scent fingerprint of hepatocarcinoma: in-vitro metastasis prediction with volatile organic compounds (VOCs)

Amal H, Ding L, Liu BB, Tisch U, Xu ZQ, Shi DY, Zhao Y, Chen J, Sun RX, Liu H, Ye SL, Tang ZY, Haick H

International Journal of Nanomedicine 2012, 7:4135-4146

Published Date: 30 July 2012

Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability

Luo H, Jiang B, Li B, Li Z, Jiang BH, Chen YC

International Journal of Nanomedicine 2012, 7:3951-3959

Published Date: 24 July 2012

Evaluation of the genotoxicity of cellulose nanofibers

de Lima R, Feitosa LO, Maruyama CR, Barga MA, Yamawaki PC, Vieira IJ, Teixeira EM, Corrêa AC, Mattoso LH, Fraceto LF

International Journal of Nanomedicine 2012, 7:3555-3565

Published Date: 11 July 2012

Multi-dye theranostic nanoparticle platform for bioimaging and cancer therapy

Singh AK, Hahn MA, Gutwein LG, Rule MC, Knapik JA, Moudgil BM, Grobmyer SR, Brown SC

International Journal of Nanomedicine 2012, 7:2739-2750

Published Date: 1 June 2012

Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups

Hong SC, Lee JH, Lee J, Kim HY, Park JY, Cho J, Lee J, Han DW

International Journal of Nanomedicine 2011, 6:3219-3231

Published Date: 7 December 2011