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Preparation and in vitro antitumor effects of cytosine arabinoside-loaded genipin-poly-L-glutamic acid-modified bacterial magnetosomes

Authors Liu YG, Dai QL, Wang SB, Deng QJ, Wu WG, Chen AZ

Received 20 October 2014

Accepted for publication 22 December 2014

Published 17 February 2015 Volume 2015:10(1) Pages 1387—1397

DOI https://doi.org/10.2147/IJN.S76123

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Lei Yang

Yuan-Gang Liu,1,2 Qing-Lei Dai,1 Shi-Bin Wang,1,2 Qiong-Jia Deng,1 Wen-Guo Wu,1,2 Ai-Zheng Chen1,2

1College of Chemical Engineering, 2Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, People’s Republic of China

Abstract: To solve the problem of synthesized magnetic nanoparticles in cancer therapy, a new drug delivery system synthesized from bacteria was used to load cytosine arabinoside (Ara-C). Genipin (GP) and poly-l-glutamic acid (PLGA) were selected as dual cross-linkers. The preparation and characterization of Ara-C-loaded GP-PLGA-modified bacterial magnetosomes (BMs) (ABMs-P), as well as their in vitro antitumor effects, were all investigated. Transmission electron micrographs (TEM) and Fourier transform infrared (FTIR) spectroscopy suggested that Ara-C could be bound to the membrane of BMs modified by GP-PLGA. The diameters of the BMs and ABMs-P were 42.0±8.6 nm and 74.9±8.2 nm, respectively. The zeta potential revealed that the nanoparticles were stable. Moreover, this system exhibited optimal drug-loading properties and long-term release behavior. The optimal encapsulation efficiency and drug-loading were 64.1%±6.6% and 38.9%±2.4%, respectively, and ABMs-P could effectively release 90% Ara-C within 40 days, without the release of an initial burst. In addition, in vitro antitumor experiments elucidated that ABMs-P is cytotoxic to HL-60 cell lines, with an inhibition rate of 95%. The method of coupling drugs on BMs using dual cross-linkers is effective, and our results reveal that this new system has potential applications for drug delivery in the future.

Keywords: magnetosomes, dual cross-linkers, nanoparticle, drug delivery

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