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Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Authors da Silva LC, Garcia T, Mori M, Sandri G, Bonferoni MC, Finotelli PV, Cinelli LP, Caramella C, Cabral LM

Received 9 March 2012

Accepted for publication 14 April 2012

Published 18 June 2012 Volume 2012:7 Pages 2975—2986

DOI https://doi.org/10.2147/IJN.S31632

Review by Single-blind

Peer reviewer comments 4

Luiz Cláudio da Silva,1 Thiago Garcia,1 Michela Mori,2 Giuseppina Sandri,2 Maria Cristina Bonferoni,2 Priscila V Finotelli,1 Leonardo P Cinelli,1 Carla Caramella,2 Lúcio M Cabral1

1Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil; 2Department of Drug Sciences, University of Pavia, Pavia, Italy

Abstract: The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.

Keywords: nanoparticles, chitosan, chondroitin sulfate, fucoidan, aPTT, Caco-2

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