Polymorphism of IFN-γ+874T/A associated with production of IFN-γ affects human papillomavirus susceptibility in rural women from Luohe, Henan, China
Authors Zhang QW, Song JY, Yu JH, Sun MZ, Tang SY, Yang SZ, Cao LJ, Wang HF, Cui LN, Fu XH
Received 4 January 2018
Accepted for publication 24 May 2018
Published 25 July 2018 Volume 2018:11 Pages 4339—4344
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication: Dr Faris Farassati
Qing-wei Zhang,1 Jia-yu Song,2 Jiang-hua Yu,1 Ming-zhen Sun,2 Si-yuan Tang,1 Shao-zhe Yang,1 Lei-jia Cao,1 Hui-fen Wang,1 Li-na Cui,1 Xiu-hong Fu1
1Department of Obstetrics and Gynaecology, Luohe Central Hospital, Luohe, 462000, Henan, China; 2Department of Pharmacology, Luohe Medical College, Luohe, 462000, Henan, China
Purpose: In this paper, the association between polymorphisms of IFN-γ+874T/A (rs2430561), IFN-γR1 -56 T/C (rs2234711), IFN-γR1 +95 C/T (rs7749390), and IFN-γR1 -611A/G (rs 1327474) and human papillomavirus (HPV) susceptibility was investigated in rural women from Luohe, Henan, China.
Patients and methods: A total of 520 rural women were enrolled from Luohe, including 260 with HPV infection and mild dysplasia or less and 260 without HPV infection. Single-nucleotide polymorphisms (SNPs) of IFN-γ +874T/A, IFN-γR1 -56 T/C, IFN-γR1 +95 C/T and IFN-γR1 -611A/G were genotyped using TaqMan Pre-Designed SNP Genotyping Assays. Serum IFN-γ levels were measured using Human IFN-γ Quantikine ELISA Kit. Multivariate logistic regression analysis was performed to identify the SNPs associated with HPV susceptibility. Serum IFN-γ levels were compared between different genotypes.
Results: The polymorphism of IFN-γ +874T/A was associated with HPV susceptibility and +874A carriers had an increased risk. Moreover, the odds ratio was higher in +874 AA carriers than in +874 AT carriers (1.672 vs 2.874). Serum IFN-γ levels were highest in IFN-γ +874 TT carriers, intermediate in AT carriers, and lowest in AA carriers (2.86±1.14 vs 1.57±0.79 vs 0.41±0.22 pg/mL, all P<0.05).
Conclusion: The polymorphism of IFN-γ +874T/A was associated with HPV susceptibility in rural women from Luohe, Henan, China, and +874A carriers had an increased risk. The possible mechanism was that +874A carriers had a low production of IFN-γ.
Keywords: single-nucleotide polymorphisms, IFN-γ, IFN-γR1, human papillomavirus, susceptibility
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