Physcion Protects Rats Against Cerebral Ischemia-Reperfusion Injury via Inhibition of TLR4/NF-kB Signaling Pathway
Authors Dong X, Wang L, Song G, Cai X, Wang W, Chen J, Wang G
Received 15 June 2020
Accepted for publication 17 December 2020
Published 25 January 2021 Volume 2021:15 Pages 277—287
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 4
Editor who approved publication: Dr Tuo Deng
Xiaobo Dong,* Lei Wang,* Guangrong Song, Xu Cai, Wenxin Wang, Jiaqi Chen, Gesheng Wang
The Third Department of Encephalopathy, Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Gesheng Wang
The Third Department of Encephalopathy, Dongfang Hospital Beijing University of Chinese Medicine, No. 6 Zone 1 Fangxingyuan, Fangzhuang, Fengtai District, Beijing 100078, People’s Republic of China
Background: Ischemic stroke (IS) is characterized by the rapid loss of brain function due to ischemia. Physcion has been found to have a neuroprotective effect against cerebral ischemia-reperfusion (I/R) injury. However, the mechanism by which physcion regulates cerebral I/R injury remains largely unknown.
Methods: An oxygen-glucose deprivation/reperfusion (OGD/R) model in SH-SY5Y cells and a rat cerebral ischemia-reperfusion (I/R) model were established, respectively. CCK-8 and flow cytometry assays were used to detect the viability and apoptosis of SH-SY5Y cells. Moreover, enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of SOD, MDA, GSH-Px, TNF-α, IL-1β, IL-6 and IL-10 in the supernatant of SH-SY5Y cells. Meanwhile, Western blot assay was used to detect the expressions of TLR4, p-p65 and p-IκB in SH-SY5Y cells and I/R rats.
Results: In this study, physcion treatment significantly rescued OGD/R-induced neuronal injury. In addition, physcion decreased inflammatory response in SH-SY5Y cells after OGD/R insult, as shown by the decreased levels of the pro-inflammatory factors TNF-α, IL-1β, IL-6 and IL-10. Moreover, physcion attenuated the oxidative stress in OGD/R-treated SY-SY5Y cells, as evidenced by the increased SOD and GSH levels and the decreased ROS and MDA levels. Meanwhile, physcion significantly reduced cerebral infarction, attenuated neuronal injury and apoptosis in I/R rats. Furthermore, physcion markedly decreased the expressions of TLR4, p-NF-κB p65 and p-IκB in the brain tissues of rats subjected to I/R and in SH-SY5Y cells exposed to OGD/R.
Conclusion: In conclusion, our study indicated that physcion protected neuron cells against I/R injury in vitro and in vivo by inhibition of the TLR4/NF-kB pathway; thus, physcion might serve as a promising therapeutic candidate for IS.
Keywords: stroke, ischemia-reperfusion injury, physcion, NF-κB
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