Phosphoglycerate dehydrogenase is a novel predictor for poor prognosis in gastric cancer
Authors Xian Y, Zhang S, Wang X, Qin J, Wang W, Wu H
Received 4 February 2016
Accepted for publication 22 June 2016
Published 7 September 2016 Volume 2016:9 Pages 5553—5560
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Triparna Sen
Peer reviewer comments 3
Editor who approved publication: Professor Jianmin Xu
Yun Xian,1,* Shu Zhang,2,* Xudong Wang,3 Jin Qin,2 Wei Wang,2 Han Wu4
1School of Public Health, Nantong University, 2Department of Pathology, 3Department of Laboratory Medicine, 4Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, People’s Republic of China
*These authors contributed equally to this work
Purpose: Phosphoglycerate dehydrogenase (PHGDH) acts as a key metabolic enzyme in the rate-limiting step in serine biosynthesis and plays an important role in metastasis of several cancers. The aim of this study was to investigate the prognostic value of PHGDH in gastric cancer (GC).
Methods: The messenger RNA expression of PHGDH was determined in 20 pairs of cancerous and adjacent nontumor tissues by real-time polymerase chain reaction. Immunohistochemistry of PHGDH was performed on tissue microarray, composed of 482 GC and 64 matched adjacent nontumor tissues acquired from surgery, 20 chronic gastritis, 18 intestinal metaplasia, and 31 low-grade and 66 high-grade intraepithelial neoplasias acquired through gastric endoscopic biopsy. Univariate and multivariate Cox proportional hazard models were used to perform survival analyses.
Results: Both PHGDH messenger RNA and protein product exhibited GC tissue-preferred expression, when compared with benign tissues. The high PHGDH expression was significantly correlated with histological type (P=0.011), tumor stage (P=0.014), and preoperative carcinoembryonic antigen (P<0.001). A negative correlation was found between PHGDH expression and the 5-year survival rate of patients with GC. Furthermore, multivariate analysis indicated that PHGDH was an independent prognostic factor for outcome in GC.
Conclusion: PHGDH is important in predicting patient outcomes and is a potential target for the development of therapeutic approaches to GC.
Keywords: metabolism, gastric cancer, prognosis, serine biosynthesis
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