Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

pH-Sensitive carboxymethyl chitosan-modified cationic liposomes for sorafenib and siRNA co-delivery

Authors Yao Y, Su ZH, Liang YC, Zhang N

Received 15 June 2015

Accepted for publication 14 August 2015

Published 1 October 2015 Volume 2015:10(1) Pages 6185—6198

DOI https://doi.org/10.2147/IJN.S90524

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Chang Liu

Peer reviewer comments 4

Editor who approved publication: Dr Lei Yang

Yao Yao, Zhihui Su, Yanchao Liang, Na Zhang

School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, People’s Republic of China

Abstract: Combination of chemotherapeutic drug and small interfering RNA (siRNA) can affect multiple disease pathways and has been proven effective in suppressing tumor progression. Co-delivery of drug and siRNA within a same nanocarrier is a vital means in this field. The present study aimed at the development of a pH-sensitive liposome to co-deliver drug and siRNA to tumor region. Driven by the electrostatic interaction, the pH-sensitive material, carboxymethyl chitosan (CMCS), was coated onto the surface of the cationic liposome (CL) preloaded with sorafenib (Sf) and siRNA (Si). To evaluate whether the resulting CMCS-modified Sf and siRNA co-delivery cationic liposome (CMCS-SiSf-CL) enhanced antitumor efficiency after systematic administration, in vitro and in vivo experiments were evaluated in HepG2 cells and the H22 cells-bearing Kunming mice model. The experimental results demonstrated that CMCS-SiSf-CL was able to condense siRNA efficiently and protect siRNA from being degraded by serum and RNase. The release rate of Sf from CMCS-modified liposome exhibited pH-sensitive release behavior. Furthermore, in vitro cellular uptake results showed that CMCS-SiSf-CL yielded higher fluorescence intensity at pH 6.5 than at pH 7.4, and that siRNA could be delivered to tumor site by CMCS-SiSf-CL in vivo. The in vivo antitumor efficacy showed that CMCS-Sf-CL inhibits tumor growth effectively when compared with free Sf solution. In current experimental conditions, this liposomal formulation did not show significant toxicity both in vitro and in vivo. Therefore, co-delivering Sf with siRNA by CMCS-SiSf-CL might provide a promising approach for tumor therapy.

Keywords: co-delivery, sorafenib, gene, charge conversion, cancer therapy

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Design, synthesis, and evaluation of VEGFR-targeted macromolecular MRI contrast agent based on biotin–avidin-specific binding

Liu YJ, Wu XY, Sun XH, Wang D, Zhong Y, Jiang DD, Wang TQ, Yu DX, Zhang N

International Journal of Nanomedicine 2017, 12:5039-5052

Published Date: 14 July 2017

Efficient co-delivery of immiscible hydrophilic/hydrophobic chemotherapeutics by lipid emulsions for improved treatment of cancer

Zhang B, Song YM, Wang TQ, Yang SM, Zhang J, Liu YJ, Zhang N, Garg S

International Journal of Nanomedicine 2017, 12:2871-2886

Published Date: 7 April 2017

A facile route to form self-carried redox-responsive vorinostat nanodrug for effective solid tumor therapy

Han LQ, Wang TQ, Wu JL, Yin XL, Fang H, Zhang N

International Journal of Nanomedicine 2016, 11:6003-6022

Published Date: 11 November 2016

Preclinical studies of N3-O-toluyl-fluorouracil-loaded lipid-based nanosuspensions in H22-bearing mice

Zhang J, Li M, Liu Z, Wang L, Liu Y, Zhang N

International Journal of Nanomedicine 2014, 9:2741-2751

Published Date: 29 May 2014

How nanotechnology can enhance docetaxel therapy

Zhang L, Zhang N

International Journal of Nanomedicine 2013, 8:2927-2941

Published Date: 7 August 2013

Folate-targeted docetaxel-lipid-based-nanosuspensions for active-targeted cancer therapy

Wang L, Li M, Zhang N

International Journal of Nanomedicine 2012, 7:3281-3294

Published Date: 29 June 2012

A pH-sensitive multifunctional gene carrier assembled via layer-by-layer technique for efficient gene delivery

Li P, Liu DH, Miao L, Liu CX, Sun XL, Liu YJ, Zhang N

International Journal of Nanomedicine 2012, 7:925-939

Published Date: 21 February 2012

Readers of this article also read:

An efficient PEGylated liposomal nanocarrier containing cell-penetrating peptide and pH-sensitive hydrazone bond for enhancing tumor-targeted drug delivery

Ding Y, Sun D, Wang GL, Yang HG, Xu HF, Chen JH, Xie Y, Wang ZQ

International Journal of Nanomedicine 2015, 10:6199-6214

Published Date: 1 October 2015

Green synthesis of water-soluble nontoxic polymeric nanocomposites containing silver nanoparticles

Prozorova GF, Pozdnyakov AS, Kuznetsova NP, Korzhova SA, Emel’yanov AI, Ermakova TG, Fadeeva TV, Sosedova LM

International Journal of Nanomedicine 2014, 9:1883-1889

Published Date: 16 April 2014

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010