Peptide crosslinked micelles: a new strategy for the design and synthesis of peptide vaccines

Jihua Hao¹, Marcin Kwissa², Bali Pulendran², Niren Murthy¹

¹Georgia Institute of Technology, Department of Biomedical Engineering, Atlanta, GA, USA; ²Emory Vaccine Center, Department of Pathology, Atlanta, GA, USA

Abstract: This report presents a new and simple methodology for the synthesis of multicomponent peptide vaccines, named the peptide crosslinked micelles (PCMs). The PCMs are core shell micelles designed to deliver peptide antigens and immunostimulatory DNA to antigen-presenting cells (APCs). They are composed of immunostimulatory DNA, peptide antigen, and a thiopyridal derived poly(ethylene glycol)-polylysine block copolymer. The peptide antigen acts as a crosslinker in the PCM strategy, which allows the peptide antigen to be efficiently encapsulated into the PCMs and also stabilizes them against degradation by serum components. Cell culture studies demonstrated that the PCMs greatly enhance the uptake of peptide antigens into human dendritic cells.

Keywords: composite, crosslinking, peptide, vaccine