Penetration Enhancing of an Erythrocyte-Mimicking Nanoplatform via Papaverine for Radiosensitization
Received 16 July 2021
Accepted for publication 27 September 2021
Published 12 October 2021 Volume 2021:16 Pages 6923—6935
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Prof. Dr. Dongwoo Khang
Jinrui Zhang,1 Huaicheng Wang,1 Weilong Suo,2 Zhizhou Li,1 Chunxu Yang3
1Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, 130033, People’s Republic of China; 2Key Laboratory of Applied Chemistry and Nanotechnology at Universities of Jilin Province, Changchun University of Science and Technology, Changchun, 130022, People’s Republic of China; 3Department of Radiation and Medical Oncology, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Zhongnan Hospital of Wuhan University, Wuhan, 430071, People’s Republic of China
Correspondence: Zhizhou Li; Chunxu Yang Email [email protected]; [email protected]
Purpose: Radiotherapy (RT) is recommended as an extensive therapeutic regimen for cancer patients; however, cancer radio-resistance results from reduced oxygen levels (hypoxia) in the tumor microenvironment. Herein, we report a therapeutic strategy that greatly enhances the treatment effects of RT.
Methods: Specifically, papaverine (ppv), an FDA-approved smooth muscle relaxant, was applied in the strategy. Ppv improved blood flow via vasodilation to deliver sufficient oxygen to the hypoxic solid tumor and further resulted in increased tumor penetration of the radiosensitizer, significantly enhancing the radiosensitization compared with no ppv treatment. Additionally, tantalum oxide nanospheres were cloaked in red blood cell membranes ([email protected]) to achieve greater biocompatibility, non-immunogenicity, and a longer circulation time.
Results: As a high-Z element, tantalum provides localized dose enhancement and thereby boosts the efficacy of RT. Vasodilation, the oxygenation of cancer cells, and the improved accumulation and retention of [email protected] in the tumor region were verified in vivo. Furthermore, compared with RT alone, the combined vasodilation and nanosphere camouflaging strategy more efficiently suppressed the growth of K7M2 tumors in mice.
Conclusion: The results of this study suggest that the integration of [email protected] and ppv has excellent potential for improving RT efficacy.
Keywords: radiotherapy, hypoxia, tantalum oxide, papaverine, vessel vasodilation
Because of radio-resistance, radiotherapy (RT) is limited as a palliative regimen for osteosarcoma cancer and as a local control.1,2 At least one factor accounts for radio-resistance: aberrant angiogenesis, which is due to the excessive proliferation of tumors cells,3 and the resulting lack of oxygen stabilizes the DNA double-strand breaks.4–6 In this respect, methods of relieving hypoxia are attracting attention for improving the effectiveness of RT.7–10 Although, overall, the mechanisms of resistance to RT are complex and remain unclear.
In order to avoid acute or chronic radiation effects, it is crucial that we identify a therapeutic strategy to enhance the deposition of radiation energy at the tumor target and to alleviate side effects in adjacent tissues or vulnerable organs.11 To promote the sensitivity of cancer cells to radiation, the use of elements with high atomic numbers for generating photo/auger electrons has been recommended.12 For instance, gold,13,14 bismuth,15,16 platinum,17 and tantalum18,19 nanoplatforms have been assessed as radiosensitizers. Despite substantial recent advances, difficulties with enhancing radiosensitization remain. Important issues include increasing the ability of the radiosensitizer to penetrate the tumor and reach all viable cells and assisting the radiosensitizer to escape clearance by the reticuloendothelial system (RES).5,20–22
Nevertheless, most tumors tend to “outgrow” the blood supply as a result of rapid cellular proliferation. To meet the demand of cancerous cells for nutrition and oxygen, angiogenesis is stimulated and new primitive blood vessels are formed, resulting in large interstitial spaces among the vessels, which often results in cancer cells being distant from blood vessels. Within tumor interstitial spaces, the dense extracellular matrix comprises a hydrophilic gel with a high ratio of collagen and elastic fiber, proteoglycans, and glycosaminoglycan, and it has an elevated fluid pressure because of heterogeneity.23,24 Meanwhile, the primitive tumor vasculature is morphologically and functionally abnormal and causes disrupted blood flow. The large interstitial space, dense extracellular matrix, and abnormal angiogenesis in tumors decrease the nanoparticle diffusion rate. Meanwhile, these factors increase the distance nanoparticles have to travel, and hinder their penetration into solid tumors, causing intratumoral diffusion resistance which cause uneven distribution of radiosensitizers.21 The nanoparticle’s limited ability to penetrate the tumor and access all tumor stem cells compromises the efficiency of the radiosensitizer, which can allow the tumor to regenerate and lead to recurrence. Therefore, attention should be given to improving nanoparticle penetration and cellular uptake in tumorous regions.25,26 Some drugs increase blood flow perfusion and, therefore, permeabilize tumor blood vessels to enhance the penetration and accumulation of nanoparticles.24,26–32 For example, iRGD peptide improves tissue permeability via αν-integrin and neuropilin-1.27,31 Dexamethasone, with anti-inflammatory properties, increases tumor accumulation by degradation or normalization of the extracellular matrix in the interstitial space.31 The normalization of vascular abnormalities is also essential in intratumoral transport.33–35 When endowed with outstanding permeability, radiosensitizers have the capacity to access the tumor region and efficiently penetrate the cancerous site while reducing accumulation in undesired tissues and, subsequently, enhancing the therapeutic outcome of ionizing radiation.
In this study, we proposed a strategy to enhance the therapeutic effects of ionizing radiation by improving the tumor oxygenation status and enhancing radiosensitizer delivery (Scheme 1). Specifically, we employed ppv, a benzylisoquinoline alkaloid isolated from opium poppy (papaver somniferum),36 which is a clinically approved non-selective smooth muscle relaxant for blood vessels that, therefore, improve ischemia.37,38 In clinical trials, ppv administration has been validated as an agent that increases vessel perfusion.39,40 To improve the effectiveness of therapeutics, researchers have turned to nature for design ideas: the red blood cell (RBC) membrane, which is the most abundant natural carrier in the blood,41–44 was used to camouflage the radiosensitizer tantalum oxide (TaOx), allowing it to escape immune attack.20,22,45–47 Therefore, we employed this strategy to enhance RT along with ppv-mediated vasodilation. Both photoacoustic (PA) imaging and immunohistochemistry analysis showed that tumor oxygen saturation was dramatically enhanced 1 h post-ppv injection, indicating that ppv can alleviate hypoxia in the tumor region by promoting the blood flow to the tumor. However, the permeability of tumor blood vessels can be modified to allow nanoparticles access to most cancerous cells and, hence, increase their efficacy, which was confirmed by further pharmacokinetic and TaOx biodistribution analysis. Hereby, on one hand, ppv improved the oxygen status in tumors, on the other hand, the penetration of RBC membrane cloaked TaOx was also modified. This therapy strategy shows potential for use in effectively inhibiting solid tumors by enhancing RT.
Scheme 1 Illustration of [email protected] and ppv application. With vasomodulatory activity, paperverine improves the oxygen levels in cancerous tissue and reduces under-perfused areas of the tumor, allowing for the thorough penetration and accumulation of [email protected]
Materials and Methods
Preparation of TaOx and [email protected]
TaOx nanoparticles were synthesized as described in a previous report and all the sources of chemicals were shown in supporting information 1.1.48 For the synthesis of hollow TaOx nanospheres, briefly, 1 mL of deionized water and 0.1 mL of ethanol were added to a round-bottomed flask. Then, 4 μL of tantalum ethoxide and 80 μL of anhydrous ethanol were mixed until homogeneous, and the mixture was added to the flask in drops. After the mixture was stirred vigorously for 30 min at room temperature, it was centrifuged for 10 min at 10,000 rpm, and the supernatant was removed. The product was washed with deionized water several times and dried in a vacuum overnight at 40 ℃.
Whole blood was withdrawn from Balb/c mice (6–8 weeks old) and stored in EDTA blood collection tubes with ethylenediamine tetraacetic acid (1.5 mg/mL) as an anticoagulant. The RBC vesicles were obtained using the low-osmosis method.49 The blood was centrifuged at 800 × g for 5 min at 4 °C to remove the plasma and the buffy coat. The resulting RBCs were washed three times with ice-cold 1 × PBS. Then, 0.25 × PBS (PBS: deionized water = 1:3) was added for hemolysis using a hypotonic medium treatment in an ice-bath for 30 min. The released hemoglobin was removed by centrifugation at 15 00 g for 5 min, and the RBC-vesicles (light pink precipitate) were collected and washed twice with 1 × PBS.
To encapsulate the TaOx, RBC-vesicles derived from 10 μL of mouse blood were first mixed with 50 μg of TaOx in 1 mL of PBS. Subsequently, the mixture was repeatedly extruded through a 200-nm nucleopore polyester membrane on a mini-extruder and then centrifuged at 1000 × g for 10 min to eliminate excess RBC-vesicles. The extruder was composed of two 10 mL syringes with needle removed. The two syringes were connected with the 200-nm nucleopore polyester membrane in the middle. Finally, the resulting RBC-TaOx were stored in PBS at 4 °C for the following experiments.
For the cell cytotoxicity assessment, K7M2 cells at the logarithmic growth phase were seeded into 96-well plates at a density of 5×103 cells per well in triplicate and incubated for 24 h at 37 ℃ with CO2 concentration of 5%. Next, the cells were incubated with [email protected] at various concentrations ranging from 25 to 200 μg/mL, and cell viability was determined using a cell counting assay (CCK 8, KeyGEN). To study the therapeutic effects of various treatment methods, 5×103 cells were seeded into 96-well plates, with three replicants per group. The Control group was set up without any treatment, and the RT group was subjected to RT (5 Gy). For the experimental groups, cells were treated with TaOx (50 μg/mL) or [email protected] (quantity of [email protected] equal to TaOx 50 μg/mL), and 12 h after the incubation, experimental cells received a radiation dose of 5 Gy. A standard CCK 8 assay was conducted to assess cell viability in each group.
The K7M2 cells were digested and seeded into 6-well plates at densities of 100, 200, 400, 800, and 1000 cells per well followed by incubation with TaOx or [email protected] After 12 h, the cells were irradiated with 2, 4, 6, or 8 Gy X-rays on a Small Animal Radiation Research Platform (PXI X-RAD 225Cx, CT, USA) with a 204-kV photon beam. After the residual nanoparticles were removed, the cells were cultured for 15 days then dyed with crystal violet. Each crystal violet dot was counted as a cell cluster. The survival curve was fitted using a “multitarget-single-hitting model”.
The animals used in this study were obtained from the Vital River Company (Beijing, China). To establish tumor-bearing models, 1×106 cells suspended in 100 μL of PBS were subcutaneously injected into the right hip of each female Balb/c mouse (aged 6–8 weeks). All the animal experiments were performed according to the protocols of the Institutional Animal Care and Use Committee (IACUC) of the Animal Experiment Center of Wuhan University (Wuhan, China) with approval number of AF146. All experiments were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Wuhan University.
Antitumor Efficacy of Combination Therapy
When the tumor volume reached 200 mm3, the mice were randomly divided into six groups and subjected to the following treatments, hereafter referred to as day 0: (1) Control; (2) RT; (3) ppv + RT; (4) [email protected] + RT; (5) TaOx + ppv + RT; (6) [email protected] + ppv + RT. The TaOx (TaOx dose: 50 mg/kg) or [email protected] (TaOx dose: 50 mg/kg) was injected into the mice via the tail vein. After 5 h, the mice in groups 5 and 6 received 2 mg/kg body weight of ppv hydrochloride by intraperitoneal injection, 1 h after which the mice in groups 3, 4, and 5 were irradiated with X-rays at a 5 Gy dose. It must be noted that ppv is unstable in saline and, thus, it was suspended in glucose carrier (5%) in this study. The tumor growth inhibition (TGI) rate was calculated using the formula: TGI = 1 − Vt/Vc, where Vt and Vc represent the average tumor volume in the treated group and control group, respectively. The combined TGI of a group a and group b was determined using the formula: TGI = 1 − (Va·Vb)/Vc2, where Va and Vb represent the average tumor volume in the treated group and control group, respectively. The tumor growth and body weight were monitored every other day until the mice were sacrificed on day 15. The tumors were surgically resected, and the tumors were weighed.
Photoacoustic Imaging of Oxygenation
The vascular blood saturation (sO2) of the solid tumors was measured on a VevoLAZR system (Fujifilm, Visualsonics Inc. Canada). Female Balb/c mice with a tumor volume of approximately 200 mm3 were selected for in vivo imaging. For observation, the hair on the right hip of the mice was removed. After the mice were anesthetized with 2% isoflurane and fixed on the platform of the photoacoustic (PA) imaging system, a layer of ultrasonic coupling gel was applied to the skin for observation. The PA images of mice before and 30 min, 1 h, 6 h and 12 h after injection of ppv (2 mg/kg body weight) and sO2 in the tumor sites were recorded.
[email protected] (TaOx dose: 50 mg/kg) suspended in PBS was intravenously injected into the K7M2-bearing mice before administration of ppv (2 mg/kg body weight) or saline through intraperitoneal injection 5 h later. Then, pimonidazole hydrochloride was intraperitoneally injected at the concentration of 60 mg per kg of body weight. After 90 min, the tumors were resected, frozen, and stained, and the images were visualized using confocal microscopy.
Pharmacokinetics and Biodistribution
We injected TaOx, [email protected], or [email protected] + ppv into Balb/c mice via the tail vein in triplicate. For the [email protected] + ppv group, the [email protected] (TaOx dose: 50 mg/kg) was administered 5 h before injecting ppv (2 mg/kg body weight). Blood samples were collected from an eye socket using EDTA blood collection tubes at 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, and 24 h post-injection. After the samples were digested in concentrated HNO3, the concentration of tantalum in all tissues was measured using Inductively coupled plasma mass spectrometry (ICP-MS). Pharmacokinetic parameters were calculated to fit with a two-compartmental model which is the most common model for describing the disposition of intravenously administered medicine using Graphpad Prism 8. The mice were euthanized at 24 h post-injection. The main organs, including the hearts, livers, spleens, lungs, and kidneys, as well as tumors were harvested and dissolved in concentrated HNO3. The tantalum content was analyzed using ICP-MS.
Hematological Evaluation and Histological Examination
The 6 to 8-week old mice were administered [email protected] (TaOx dose: 50 mg/kg) + ppv (2 mg/kg body weight). Blood samples were collected for analysis before the mice were dissected 30 days after injection. The main organs, including the heart, liver, spleen, lung, and kidney, were sectioned and stained with hematoxylin-eosin. An inverted laboratory microscope was used to capture images.
Data are presented as mean ± standard deviation (SD). Statistical differences were assessed using the student’s t-test. P-values of <0.05 were considered statistically significant, and * means P < 0.05, ** means P < 0.01, and *** means P < 0.005.
Results and Discussion
Synthesis and Characterization of [email protected]
To prepare the [email protected], hollow TaOx was first synthesized via the one-pot method by the simple addition of tantalum (V) ethoxide to a mixture of water and ethanol at room temperature, as previously reported.19 Next, the TaOx was coated with RBC membranes using an extrusion method. The transmission electron microscopy (TEM) images (Figure 1A and B) show the uniform hollow structure of the TaOx and the core-shell structure of [email protected] with a thin lipid bilayer. Further investigation using high-angle annular dark-field scanning TEM (HAADF-STEM) confirmed the presence of a TaOx core with a diameter of 85.6 nm and a 6.8-nm-thick membrane shell (Figure 1C), indicating a lipid bilayer thickness of approximately 7 nm, which is consistent with the RBC membrane width. Additionally, the measurement of dynamic light scattering gave an average diameter of 97.1 nm and 106.2 nm for TaOx and [email protected], respectively (Figure 1D). In addition, zeta-potential measurements (Figure 1E) exhibited a shift in the zeta-potential from −20.1 mV (TaOx) to −26.8 mV ([email protected]), which are close to the zeta-potential of RBC vesicles (−33.2 mV). Further SDS-PAGE analysis whose experiment detail was shown in supporting information 1.3 verified the maintenance of most cell membrane components in [email protected] (Figure 1F), indicating that membrane-associated protein functions were preserved. The above results demonstrated that TaOx was successfully cloaked with the RBC membrane. In contrast to TaOx, which showed low stability in physiological solutions, [email protected] exhibited superior stability in both saline and serum for up to 24 h (Figure S1 as shown in the supporting information file). All the instruments were shown in supporting information 1.2.
Figure 1 Characterization of [email protected] nanoparticles. (A, B) TEM images of TaOx and [email protected], respectively. (C) HAADF-STEM image and elemental mapping of TaOx. (D) Size distribution of RBC membrane, TaOx, and [email protected] (E) Surface zeta-potentials of TaOx and [email protected], respectively. (F) Proteins in RBC membrane; TaOx, and [email protected] were solubilized and resolved on a polyacrylamide gel.
Radiosensitization in vitro
First, the hematolysis rate of [email protected] was less than 5%, even at a concentration of 200 μg/mL, indicating there was no obvious risk of damage to the erythrocytes (Figure S2 as shown in the supporting information file). We evaluated the biocompatibility of TaOx and [email protected] using a cell counting kit (CCK) 8 assay following the cell culture method in supporting information 1.4. As shown in Figure S3 as shown in the supporting information file, both TaOx and [email protected] exhibited minimal cytotoxicity to K7M2, even at high concentrations, suggesting the biomedical application of TaOx and [email protected] is feasible. TaOx can act as a radiosensitizer because of its high atomic number, according to a previously reported study.19 Next, we compared the radiosensitizing effect of [email protected] under both hypoxic and normoxic conditions. γ-H2AX is a marker of DNA double-strand breaks. As shown in Figure 2A and B, cells pretreated with [email protected] exhibited higher levels of DNA damage than those treated with RT alone in normoxic conditions. However, because oxygen is indispensable in preventing the repair of DNA radicals, the DNA damage was highly suppressed under hypoxic conditions, whether the cells were incubated with [email protected] or not. Cells treated with [email protected] prior to irradiation with 5 Gy showed a decrease in cell viability with an increase in the concentration of [email protected] under normoxia; in contrast, cell viability was consistently high in hypoxic conditions at all concentrations (Figure 2C). The above results demonstrated that [email protected] possesses remarkable radiosensitization ability in normoxia but this ability was limited in hypoxia situation, which is common in solid tumors. A clonogenic assay was used as a “gold standard” to evaluate the proliferation ability of cancerous cells (Figure 2D). Under hypoxic conditions, both RT and [email protected] + RT exhibited a limited inhibitory effect on cell proliferation. In comparison, there was an obvious separation between the [email protected] + RT curve under normoxic conditions and that under hypoxic conditions, which highlights the importance of oxygen during RT. The sensitizing enhancement ratio, which is representative of enhancement efficiency, was calculated to be 1.93 for [email protected] under normoxia, indicating that the RBC-mimicking TaOx effectively radiosensitized K7M2 cells.
Figure 2 In vitro radiosensitization with [email protected] (A) Confocal fluorescence image of γ-H2AX-stained K7M2 cells treated with PBS or [email protected] (50 μg/mL) with or without radiation (5 Gy). (B) Quantitative analysis of γ-H2AX foci density (γ-H2AX foci/100 μm2) for n > 100 cells. Group 1: PBS; Group 2: RT (hypoxia); Group 3: [email protected] + RT (hypoxia); Group 4: RT (normoxia); Group 5: [email protected] + RT (normoxia). (C) Cell viabilities of K7M2 cells incubated with [email protected] at various concentrations under hypoxia or normoxia. (D) Clonogenic curves. The K7M2 cells were treated with PBS or [email protected] (50 μg/mL) and irradiated with X-rays of various dosages. All data are presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, and ***p < 0.001.
Oxygenation and Vasodilation by Ppv
In the majority of cancers, the blood vessels have abnormal structure and function, which fuel disease progression and resistance to treatment.50 Therefore, it is advantageous to optimize the blood flow in tumors. Considering the potent vasodilatory effect of ppv accelerates blood flow, the intermittent blood flow induced by the abnormal vasculature hinders the delivery of oxygen to tumorous tissues. Because the velocity of the blood flow is proportional to vessel diameter, the blood flow and oxygen status may be altered after the injection of ppv.51 To validate the hypoxia-alleviating capability of ppv, in vivo PA imaging was performed on living mice bearing K7M2 tumors to observe the vascular blood saturation (sO2) in real-time. As illustrated in Figure 3A, PA signals at the tumor sites gradually increased and reached the maximum at 1 h post-injection, with a signal intensity 5.3-fold that before injection and the PA signal sustained at a relative high intensity at 12 h post the injection (Figure 3B). Because ppv improved the vascular blood flow and, therefore, increased the intratumor blood oxygen saturation, it was highly beneficial in relieving hypoxia. To further verify its hypoxia alleviation effectiveness, tumors of mice that were concurrently treated with ppv were sectioned for HIF-1α immunofluorescent (green fluorescent) staining. As shown in Figure 3C, the expression levels of the HIF-1α protein in tumors significantly decreased over time after the injection of ppv, demonstrating the potent ability of ppv to relieve hypoxia. The intensity of HIF-1α fluorescence at 30 min post-injection was 5.2-fold that before injection (Figure 3D), which was due to the elevated oxygen content in the tumorous region. Further investigation on hypoxia status of 0 h, 0.5 h and 1 h post the injection of ppv was shown in Figure S4. It can be inferred that 1 h post the injection the hypoxia status was alleviated to the largest extent among these three time points. All the results so far suggest that ppv alleviated tumor hypoxia by accelerating blood flow.
Ppv Facilitates [email protected] Accumulation and Tumor Penetration
Following our demonstration of the promising effects of ppv in vivo, we further investigated whether ppv has an impact on preferential [email protected] accumulation and penetration at a systemic level. The K7M2 tumor-bearing mice were i.v. injected with TaOx (Group 1), [email protected] (Group 2), or ppv + [email protected] (Group 3). Following animal sacrifice, we studied the pharmacokinetic profiles of the three groups. Both Group 2 and 3 showed a prolonged blood circulation half-time relative to Group 1, which was attributed to the cloaking of TaOx by the RBC membrane (Figure 4A). The distribution of tantalum in the major organs and tumors was measured (Figure 4B) and, for all groups, the majority of nanoparticles were in the liver and spleen – two primary organs of the RES. Tantalum contents were lower in the liver and spleen and higher in the tumors of mice treated with [email protected] compared with those treated with TaOx, because the erythrocyte coat was “invisible” to the immune system. However, Group 3 showed the highest accumulation of tantalum in tumorous tissues as well as the lowest tantalum contents in the liver and spleen compared with the other groups. The above results suggest that ppv enables TaOx accumulation within tumors and prevents its accumulation in the liver and spleen. Therefore, nanoparticles are cleared more slowly from tumor tissues, increasing the efficacy of the RT. Next, to compare the effect of ppv on biodistribution, dye-labeled [email protected] were injected via tail vein and ex vivo imaging was conducted to confirm the biodistribution. In [email protected] group, ppv was administrated before [email protected] As shown in Figure 4C and D, ppv+ [email protected] group exhibited higher signal intensity than that of [email protected] group, which was consistent with the result in vivo. Moreover, we monitored the tissue accumulation at tissue level. As shown in Figure 4E, more DiO-labeled [email protected] were found in [email protected] group however lower green fluorescence signals were observed in [email protected], which was identified with the quantitative results of fluorescence intensity in Figure 4F. It should be notified that the ppv is capable of improving the penetration ability of nanoparticles. These results clearly verified the importance of ppv on enhancing accumulation of [email protected] in tumor tissues.
Figure 4 Enhanced penetration and accumulation of ppv. (A) Pharmacokinetic study of the various formulations. (B) Biodistribution of tantalum in tumors and major organs after various treatments. (C) Ex vivo fluorescence imaging and (D) quantitative data of fluorescence signals in main organs and tumors. (E) Immunofluorescence staining imaging of the intratumoral distribution of DiO-labeled [email protected], cell nuclei was stained with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (blue) and [email protected] was labeled with DiO (green) and (F) quantitative analysis of fluorescence signals of Dio-labeled [email protected] All data are presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, and ***p < 0.001.
Before studying the antitumor treatments in vivo, their biosafety was assessed in Balb/c mice. In order to investigate the risk of ppv and [email protected] may pose to healthy tissues in a relative long term, we assessed the hematological parameters of mice 30 days after treatment with [email protected] and ppv. As shown in Figure 5A, there were negligible differences among the groups. Moreover, histological examination revealed no apparent lesions or apoptosis in the main organs in any of the groups (Figure 5B). Collectively, ppv and [email protected] showed no significant toxicity on the organs.
Figure 5 In vivo toxicity evaluations. (A) Blood biochemistry analysis after various treatments. Blue: control group; red: ppv + [email protected] group. (B) Micrographs of hematoxylin and eosin staining of main organs after various treatments.
To verify the effectiveness of using ppv prior to [email protected] for radiosensitization, mice were divided randomly into five groups: Group 1: saline control; Group 2: RT only; Group 3: ppv + RT; Group 4: [email protected] + RT; Group 5: ppv + TaOx + RT; and Group 6: ppv + [email protected] + RT. The mice were treated with or without ppv after the administration of TaOx or [email protected], followed by X-ray irradiation at 5 h post-injection. The treatment procedure for Group 6 is provided as an example in Figure 6A. There was no obvious difference in body weights among the groups (Figure 6B). As shown in Figure 6C, RT alone did not effectively suppress the proliferation of tumors, whereas a slightly better tumor inhibition of 54.3% was achieved when [email protected] was introduced as a radiosensitizer. Consistent with a previously reported study, which showed that ppv and its derivatives enhanced the response of solid tumors to RT, ppv + RT showed 46.6% TGI. Moreover, the ppv + TaOx + RT group exhibited a high TGI ratio of 65.7%. Notably, ppv + [email protected] + RT showed the most remarkable tumor growth inhibition, with a dramatic increase in the TGI ratio (87.3%), a 1.61-fold increase from that of Group 4; hence, the administration of ppv after the radiosensitizer led to significantly enhanced RT. In addition, the TGI in Group 6 was higher than the TGIs (75.6%) of groups 3 and 4 combined, indicating that the therapy had a synergistic effect. Moreover, we observed a stronger antitumor effect from ppv + [email protected] + RT compared with ppv + TaOx + RT as a result of the camouflage effect of the erythrocyte membrane. Analysis of the tumor weights (Figure 6D) further confirmed that ppv + [email protected] + RT generated the most effective tumor inhibition effect of all the treatments. Compared with the other treatments, which exhibited constrained tumor suppression, ppv + [email protected] + RT resulted in severe structural disruption as well as the highest level of tumor apoptosis according to the histological analysis and TUNEL staining (Figure 6E).
Figure 6 In vivo therapy evaluation. (A) Illustration of therapy procedure. Changes in (B) body weight, (C) tumor volume, and (D) tumor weight of mice in various groups. Red: control group; Orange: RT group; gray: ppv + RT; purple: [email protected] + RT; blue: ppv + TaOx + RT; green: ppv + [email protected] + RT. (E) Histological TUNEL and H&E staining of sections of tumor slices after various treatments. All data are presented as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, and ***p < 0.001.
We showed the feasibility of applying ppv injections to enhance the radiosensitization effect of [email protected] The proposed method provide possibility of improve the hypoxia status in tumor regions. The function of ppv was to induce vasodilation, which accelerated blood flow to improve oxygen delivery to the tumorous area, enabling the RT-induced suppression of DNA repair. Furthermore, in the in vivo experiments, ppv notably enhanced [email protected] accumulation and penetration in tumors. Additionally, the RBC membrane coating camouflaged the nanoparticle, prolonging its circulation time and improving its retention by preventing RES clearance. A significant tumor inhibition effect (87.3%) was observed when ppv and [email protected] were used in combination as a radiosensitization strategy. The strategy presented here may provide a new method of overcoming impediments to the efficacy of RT in combating tumors.
Supporting information is available from online or from the author.
We greatly acknowledge the financial support from the National Natural Science Foundation of China (No. 81901882). The authors would like to thank Shiyanjia lab (www.shiyanjia.com) for drawing schematic illustration.
The authors report no conflicts of interest in this work.
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