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Pathogenic Characteristics of and Variation in Vibrio parahaemolyticus Isolated from Acute Diarrhoeal Patients in Southeastern China from 2013 to 2017

Authors Chen X, Zhu Q, Liu Y, Wang R, Xie H, Chen J, Cheng Y, Zhang H, Cao L, Chen Y

Received 10 October 2019

Accepted for publication 14 March 2020

Published 5 May 2020 Volume 2020:13 Pages 1307—1318

DOI https://doi.org/10.2147/IDR.S234186

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Sahil Khanna


Xiao Chen,1,2,* Qiaoyun Zhu,2,* Yanchao Liu,2 Ruonan Wang,2 Hongyi Xie,3 Jiayao Chen,4 Ying Cheng,5 Haiping Zhang,6 Lijun Cao,7 Yu Chen1,2

1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 2Key Laboratory of Clinical in vitro Diagnostic Techniques of Zhejiang Province, Department of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 3Department of Laboratory Medicine, Beilun People’s Hospital, Ningbo, Zhejiang, People’s Republic of China; 4Department of Laboratory Medicine, Zhoushan People’s Hospital, Zhoushan, Zhejiang, People’s Republic of China; 5Department of Laboratory Medicine, Quzhou People’s Hospital, Quzhou, Zhejiang, People’s Republic of China; 6Department of Laboratory Medicine, Yuhuan People’s Hospital, Yuhuan, Zhejiang, People’s Republic of China; 7Department of Laboratory Medicine, People’s Hospital of Zhenhai District, Ningbo, Zhejiang, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Yu Chen
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Tel +86057187236383
Email chenyuzy@zju.edu.cn

Objective: Vibrio parahaemolyticus is a major diarrhoea-inducing pathogen in coastal areas. In this study, we analysed the pathogenic characteristics of and variation in V. parahaemolyticus isolated from acute diarrhoeal patients in seven hospitals in different areas of southeastern China from 2013 to 2017.
Methods: The fecal specimens of patients with acute diarrhoea were collected. The routine microbiological test procedure combining with MALDI Biotyper microbial identification system was carried out to identify the V. parahaemolyticus. Serum agglutination tests, PCR for the detection of virulence-related genes and the Kirby-Bauer method to test for antimicrobial sensitivity were performed.
Results: From 2013 to 2017 in southeastern China, a total of 1220 V. parahaemolyticus strains were isolated from 16,504 stool specimens collected from acute diarrhoeal patients, and the annual isolation rate fluctuated between 6.1% and 8.7%. In total, 96.7% of the V. parahaemolyticus isolates were isolated in summer and autumn, mainly in people aged 18– 44. Fifty-nine serotypes were identified, and the agglutination rate of the O antigen was 98.5%. From 2014 to 2016, the dominant serotype was O3:K6, while in 2013 and 2017, it was O4:KUT. The serotypes of O3:K6, O4:KUT, O4:K8, O3:KUT, O10:K60, O1:KUT and O1:K36 appeared every year from 2013 to 2017. O4:K6 and OUT:K6 began to appear after 2014 and 2015, respectively. A total of 49.5% of the strains belonged to the pandemic group, which consisted of 26 serotypes. Most isolates were sensitive to common antibiotics, excluding ampicillin.
Conclusion: V. parahaemolyticus is still present at a high level in southeastern China. Although the pandemic O3:K6 serotype is predominant, new serotypes continue to emerge, especially the O4:KUT serotype, which exceeded O3:K6 in prevalence in some years. Long-term surveillance is necessary to prevent the outbreak or transmission of this pathogen.

Keywords: Acute diarrhoea, Vibrio parahaemolyticus, serotypes, virulence genes, antimicrobial resistance

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